Literature summary for 4.1.2.38 extracted from

Zavrel, M.; Schmidt, T.; Michalik, C.; Ansorge-Schumacher, M.; Marquardt, W.; Buechs, J.; Spiess, A.C.
Mechanistic kinetic model for symmetric carboligations using benzaldehyde lyase (2008), Biotechnol. Bioeng., 101, 27-38.

Search for more literature for 4.1.2.38

Cloned(Commentary)

Cloned (Comment)	Organism
for expression in Escherichia coli cells	Pseudomonas fluorescens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.005	-	3,5-dimethoxy-benzaldehyde	Michaelis constant for second binding substrate, mechanistic model	Pseudomonas fluorescens
0.007	-	3,5-dimethoxy-benzaldehyde	Michaelis constant for second binding substrate, mechanistic model	Pseudomonas fluorescens
0.01	-	3,5-dimethoxy-benzaldehyde	Michaelis constant for first binding substrate, simplified model	Pseudomonas fluorescens
0.012	-	3,5-dimethoxy-benzaldehyde	Michaelis constant for first binding substrate, simplified model	Pseudomonas fluorescens
0.042	-	3,5-dimethoxy-benzaldehyde	Michaelis constant for second binding substrate, simplified model	Pseudomonas fluorescens
0.047	-	3,5-dimethoxy-benzaldehyde	Michaelis constant for second binding substrate, simplified model	Pseudomonas fluorescens
0.05	-	3,5-dimethoxy-benzaldehyde	Michaelis constant for first binding substrate, mechanistic model	Pseudomonas fluorescens
0.975	-	(R)-3,3',5,5'-tetramethoxy-benzoin	Michaelis constant for product, mechanistic model	Pseudomonas fluorescens
1.205	-	(R)-3,3',5,5'-tetramethoxy-benzoin	Michaelis constant for product, mechanistic model	Pseudomonas fluorescens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
58919	-	4 * 58919	Pseudomonas fluorescens
58920	-	monomer, calculated from sequence	Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-hydroxy-1,2-diphenylethanone	Pseudomonas fluorescens	-	benzaldehyde	-	r

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas fluorescens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using Ni2+-affinity chromatography	Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-hydroxy-1,2-diphenylethanone	-	Pseudomonas fluorescens	benzaldehyde	-	r
3,5-dimethoxy-benzaldehyde	-	Pseudomonas fluorescens	(R)-3,3',5,5'-tetramethoxy-benzoin	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 58919	Pseudomonas fluorescens

Synonyms

Synonyms	Comment	Organism
BAL	-	Pseudomonas fluorescens
benzaldehyde lyase	-	Pseudomonas fluorescens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	activity assay	Pseudomonas fluorescens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
45.44	-	3,5-dimethoxy-benzaldehyde	simplified model	Pseudomonas fluorescens
46.12	-	3,5-dimethoxy-benzaldehyde	mechanistic model	Pseudomonas fluorescens
46.15	-	3,5-dimethoxy-benzaldehyde	mechanistic model	Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	activity assay	Pseudomonas fluorescens

Cofactor

Cofactor	Comment	Organism	Structure
thiamine diphosphate	-	Pseudomonas fluorescens

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Release 2023.1 (January 2023)