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Literature summary for 4.1.2.38 extracted from
- Probing the active center of benzaldehyde lyase with substitutions and the pseudosubstrate analogue benzoylphosphonic acid methyl ester (2008), Biochemistry, 47, 7734-7743.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme is a tool in chemoenzymatic synthesis of chiral alpha-hydroxyketones | Pseudomonas fluorescens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the plasmid pKK-BAL-His is constructed for expression of wild-type BAL in Escherichia coli BL21DE3pLysS cells | Pseudomonas fluorescens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| the structure of benzaldehyde lyase in its covalent complex with methyl benzoylphosphonate is determined to 2.49 A | Pseudomonas fluorescens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H29A | the role of His29 in cofactor activation and catalysis is delineated | Pseudomonas fluorescens |
| W163A | the role of Trp163 in cofactor activation and catalysis is delineated | Pseudomonas fluorescens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Pseudomonas fluorescens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-hydroxy-1,2-diphenylethanone | Pseudomonas fluorescens | - |
benzaldehyde | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-hydroxy-1,2-diphenylethanone | - |
Pseudomonas fluorescens | benzaldehyde | - |
r | |
| methyl benzoylphosphonate | - |
Pseudomonas fluorescens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | - |
Pseudomonas fluorescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BAL | - |
Pseudomonas fluorescens |
| benzaldehyde lyase | - |
Pseudomonas fluorescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
activity assay | Pseudomonas fluorescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
activity assay | Pseudomonas fluorescens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | - |
Pseudomonas fluorescens | |
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Release 2023.1 (January 2023)
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