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Literature summary for 4.1.2.38 extracted from
- Mechanism of benzaldehyde lyase studied via thiamin diphosphate-bound intermediates and kinetic isotope effects (2008), Biochemistry, 47, 3800-3809.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the plasmid pKK-BAL-His is constructed for expression of wild-type BAL in Escherichia coli BL21DE3pLysS cells | Pseudomonas fluorescens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Pseudomonas fluorescens |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58919 | - |
4 * 58919 | Pseudomonas fluorescens |
| 58920 | - |
monomer, calculated from sequence | Pseudomonas fluorescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-hydroxy-1,2-diphenylethanone | Pseudomonas fluorescens | - |
benzaldehyde | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | P51853 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (E)-2-oxo-4(pyridin-3-yl)-3-but-3-enoic acid | - |
Pseudomonas fluorescens | (E)-3-(pyridine-3-yl)acrylaldehyde | - |
? | |
| 2-hydroxy-1,2-diphenylethanone | - |
Pseudomonas fluorescens | benzaldehyde | - |
r | |
| benzoylformic acid | - |
Pseudomonas fluorescens | ? | - |
? | |
| phenylpyruvic acid | - |
Pseudomonas fluorescens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 58919 | Pseudomonas fluorescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BAL | - |
Pseudomonas fluorescens |
| benzaldehyde lyase | - |
Pseudomonas fluorescens |
| benzoin aldolase | - |
Pseudomonas fluorescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
activity assay | Pseudomonas fluorescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
activity assay | Pseudomonas fluorescens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | - |
Pseudomonas fluorescens | |
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