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Literature summary for 4.1.2.38 extracted from

  • Dominguez de Maria, P.; Stillger, T.; Pohl, M.; Wallert, S.; Drauz, K.; Groeger, H.; Trauthwein, H.; Liese, A.
    Preparative enantioselective synthesis of benzoins and (R)-2-hydroxy-1-phenylpropanone using benzaldehyde lyase (2006), J. Mol. Catal. B, 38, 43-47.
No PubMed abstract available
Search for more literature for 4.1.2.38

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pseudomonas fluorescens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent, only in the presence of both cofactors, 0.015 mM Mg2+ and thiamine diphosphate, a significant enzymatic stabilisation is achieved Pseudomonas fluorescens

Organic Solvent Stability

Organic Solvent Comment Organism
dithiothreitol positive effect on the enzyme stability Pseudomonas fluorescens
DMSO 30% (v/v) is optimal for the enzyme activity and stability Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
 strain biovar I
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methoxybenzaldehyde
-
 Pseudomonas fluorescens (R)-1,2-di-(4-methoxy-phenyl)-2-hydroxy-ethan-1-one
-
 ?
benzaldehyde + acetaldehyde
-
 Pseudomonas fluorescens (R)-2-hydroxy-1-phenylpropanone
-
 ?
benzoin
-
 Pseudomonas fluorescens benzaldehyde + benzaldehyde
-
 r
furan-carbaldehyde
-
 Pseudomonas fluorescens (R)-1,2-difuryl-2-hydroxy-ethan-1-one
-
 ?

Synonyms

Synonyms Comment Organism
BAL
-
 Pseudomonas fluorescens
benzaldehyde lyase
-
 Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
 highest carboligation activity, 10fold increase in activity compared to pH 7.0 Pseudomonas fluorescens

pH Stability

pH Stability pH Stability Maximum Comment Organism
10
-
 sharp decrease in activity above pH 10 Pseudomonas fluorescens

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate dependent, only in the presence of both cofactors, 0.015 mM Mg2+ and thiamine diphosphate, a significant enzymatic stabilisation is achieved Pseudomonas fluorescens