Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Nocardioides sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
(E)-2'-carboxybenzylidenepyruvate | pH 7.0, 25°C | Nocardioides sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
3 * 35000, calculated from sequence | Nocardioides sp. |
38000 | - |
3 * 38000, SDS-PAGE | Nocardioides sp. |
113000 | - |
gel filtration | Nocardioides sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O | Nocardioides sp. | the enzyme is involved in the degradation of phenanthrene | 2-carboxybenzaldehyde + pyruvate | i.e.2-carboxybenzaldehyde + pyruvate | r | |
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O | Nocardioides sp. KP7 | the enzyme is involved in the degradation of phenanthrene | 2-carboxybenzaldehyde + pyruvate | i.e.2-carboxybenzaldehyde + pyruvate | r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocardioides sp. | Q79EM8 | - |
- |
Nocardioides sp. KP7 | Q79EM8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Nocardioides sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:phenanthrene-grown cell | - |
Nocardioides sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.5 | - |
- |
Nocardioides sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O | the enzyme is involved in the degradation of phenanthrene | Nocardioides sp. | 2-carboxybenzaldehyde + pyruvate | i.e.2-carboxybenzaldehyde + pyruvate | r | |
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O | i.e. 2'-carboxybenzalpyruvate, equilibrium constant is 298 | Nocardioides sp. | 2-carboxybenzaldehyde + pyruvate | i.e. 2-carboxybenzaldehyde + pyruvate | r | |
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O | the enzyme is involved in the degradation of phenanthrene | Nocardioides sp. KP7 | 2-carboxybenzaldehyde + pyruvate | i.e.2-carboxybenzaldehyde + pyruvate | r | |
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O | i.e. 2'-carboxybenzalpyruvate, equilibrium constant is 298 | Nocardioides sp. KP7 | 2-carboxybenzaldehyde + pyruvate | i.e. 2-carboxybenzaldehyde + pyruvate | r | |
additional information | no activity with trans-2'-hydroxycinnamate, benzalpyruvate, and trans-2'-methoxybenzalpyruvate | Nocardioides sp. | ? | - |
? | |
additional information | no activity with trans-2'-hydroxycinnamate, benzalpyruvate, and trans-2'-methoxybenzalpyruvate | Nocardioides sp. KP7 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 38000, SDS-PAGE | Nocardioides sp. |
trimer | 3 * 35000, calculated from sequence | Nocardioides sp. |
Synonyms | Comment | Organism |
---|---|---|
phdJ | - |
Nocardioides sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Nocardioides sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
(E)-2'-carboxybenzylidenepyruvate | pH 7.0, 25°C | Nocardioides sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Nocardioides sp. |