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Literature summary for 4.1.2.32 extracted from
- Purification and characterization of trimethylamine-N-oxide demethylase from jumbo squid (Dosidicus gigas) (2006), J. Agric. Food Chem., 54, 968-972.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | 1 mM, 17.9% increase of activity | Dosidicus gigas |  |
| EDTA | 10 mM, 94.2% increase of activity | Dosidicus gigas | |
| Na2SO3 | 10 mM, 97.4% increase of activity | Dosidicus gigas | |
| NADH | 0.4 mM, 41.7% increase of activity | Dosidicus gigas | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetate | 97.1% inhibition at 10 mM | Dosidicus gigas | |
| phytic acid | 95.6% inhibition at 10 mM | Dosidicus gigas | |
| tea polyphenol | 98.6% inhibition at 10 mM | Dosidicus gigas | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 26.2 | - |
Trimethylamine N-oxide | in 100 mM Tris-acetate (pH 7.0), 2 mM cysteine, 2 mM ascorbate, and 0.2 mM FeCl2, at 50°C | Dosidicus gigas | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM, 20.5% increase of activity | Dosidicus gigas | |
| Mg2+ | 10 mM, 28.8% increase of activity | Dosidicus gigas | |
| additional information | not affected by Fe2+ | Dosidicus gigas |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17500 | - |
SDS-PAGE | Dosidicus gigas |
| 17700 | - |
gel filtration | Dosidicus gigas |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dosidicus gigas | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| diethylaminoethyl-cellulose chromatography and Sephacryl S-300 gel filtration | Dosidicus gigas |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.19 | - |
cell extract, at 50°C | Dosidicus gigas |
| 159 | - |
after 839fold purification, at 50°C | Dosidicus gigas |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Trimethylamine N-oxide | - |
Dosidicus gigas | Dimethylamine + formaldehyde | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TMAOase | - |
Dosidicus gigas |
| trimethylamine-N-oxide demethylase | - |
Dosidicus gigas |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Dosidicus gigas |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Dosidicus gigas |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Dosidicus gigas |
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