Application | Comment | Organism |
---|---|---|
analysis | the addition of sphingosine to cultures of cell lines or primary cells provides a source of intracellular sphingosine 1-phosphate that is susceptible to degradation by the lyase and, hence, increases on inhibition or silencing of the enzyme. Development of a biochemical assay optimized with respect to sphingosine concentration, incubation time, and cell density and establishment for routine use with HEK293 cells. The assay is suitable for the testing of novel active site-directed sphongosine-1-phosphate lyase inhibitors | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-549 cell | - |
Homo sapiens | - |
HEK-293 cell | - |
Homo sapiens | - |
General Information | Comment | Organism |
---|---|---|
physiological function | silencing or inhibition of sphingosine-1-phosphate lyase with short interfering RNA or active site-directed inhibitors in cultured mammalian cells does not cause a relevant increase of sphingosine-1-phosphate in the cells. The addition of sphingosine to cultures of cell lines or primary cells provides a source of intracellular sphingosine-1-phosphate that is susceptible to degradation by the lyase and, hence, increases on inhibition or silencing of the enzyme | Homo sapiens |