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Literature summary for 4.1.2.26 extracted from

  • Giger, L.; Toscano, M.; Bouzon, M.; Marlière, P.; Hilvert, D.
    A novel genetic selection system for PLP-dependent threonine aldolases (2012), Tetrahedron, 68, 7549-7557.
No PubMed abstract available
Search for more literature for 4.1.2.26

Cloned(Commentary)

Cloned (Comment) Organism
-
 Caulobacter vibrioides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 L-threo-phenylserine wild-type, pH 8.0, 30°C Caulobacter vibrioides
0.065
-
 L-threo-phenylserine mutant D95N/E96S, pH 8.0, 30°C Caulobacter vibrioides
0.071
-
 L-threo-phenylserine mutant D95G/E96G, pH 8.0, 30°C Caulobacter vibrioides
0.17
-
 L-threo-phenylserine mutant D95C, pH 8.0, 30°C Caulobacter vibrioides
0.19
-
 L-threo-phenylserine mutant D95L, pH 8.0, 30°C Caulobacter vibrioides
0.24
-
 L-threo-phenylserine mutant D176E, pH 8.0, 30°C Caulobacter vibrioides
0.31
-
 L-threo-phenylserine mutant D95Y, pH 8.0, 30°C Caulobacter vibrioides
0.58
-
 L-threo-phenylserine mutant D95M, pH 8.0, 30°C Caulobacter vibrioides
0.78
-
 L-threo-phenylserine mutant D95W, pH 8.0, 30°C Caulobacter vibrioides
2.8
-
 L-threo-phenylserine mutant D95Y/E96T, pH 8.0, 30°C Caulobacter vibrioides

Organism

Organism UniProt Comment Textmining
Caulobacter vibrioides Q9A3V8 enzyme is a low-specificity L-threonine aldolase, EC 4.1.2.48, and additionally cleaves L-threo-phenylserine
-
Caulobacter vibrioides CB15 Q9A3V8 enzyme is a low-specificity L-threonine aldolase, EC 4.1.2.48, and additionally cleaves L-threo-phenylserine
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-threo-phenylserine
-
 Caulobacter vibrioides glycine + benzaldehyde
-
 ?
L-threo-phenylserine
-
 Caulobacter vibrioides CB15 glycine + benzaldehyde
-
 ?
additional information enzyme is a low-specificity L-threonine aldolase, EC 4.1.2.48, and additionally cleaves L-threo-phenylserine Caulobacter vibrioides ?
-
 ?
additional information enzyme is a low-specificity L-threonine aldolase, EC 4.1.2.48, and additionally cleaves L-threo-phenylserine Caulobacter vibrioides CB15 ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 L-threo-phenylserine mutant D176E, pH 8.0, 30°C Caulobacter vibrioides
0.78
-
 L-threo-phenylserine mutant D95W, pH 8.0, 30°C Caulobacter vibrioides
2.8
-
 L-threo-phenylserine mutant D95Y/E96T, pH 8.0, 30°C Caulobacter vibrioides
5.5
-
 L-threo-phenylserine mutant D95N/E96S, pH 8.0, 30°C Caulobacter vibrioides
6.8
-
 L-threo-phenylserine mutant D95G/E96G, pH 8.0, 30°C Caulobacter vibrioides
14
-
 L-threo-phenylserine mutant D95Y, pH 8.0, 30°C Caulobacter vibrioides
21
-
 L-threo-phenylserine mutant D95M, pH 8.0, 30°C Caulobacter vibrioides
22
-
 L-threo-phenylserine mutant D95C, pH 8.0, 30°C Caulobacter vibrioides
23
-
 L-threo-phenylserine mutant D95L, pH 8.0, 30°C Caulobacter vibrioides
67.4
-
 L-threo-phenylserine wild-type, pH 8.0, 30°C Caulobacter vibrioides

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.42
-
 L-threo-phenylserine mutant D176E, pH 8.0, 30°C Caulobacter vibrioides
22
-
 L-threo-phenylserine mutant D95W, pH 8.0, 30°C Caulobacter vibrioides
36
-
 L-threo-phenylserine mutant D95M, pH 8.0, 30°C Caulobacter vibrioides
45
-
 L-threo-phenylserine mutant D95Y, pH 8.0, 30°C Caulobacter vibrioides
85
-
 L-threo-phenylserine mutant D95N/E96S, pH 8.0, 30°C Caulobacter vibrioides
96
-
 L-threo-phenylserine mutant D95G/E96G, pH 8.0, 30°C Caulobacter vibrioides
100
-
 L-threo-phenylserine mutant D95Y/E96T, pH 8.0, 30°C Caulobacter vibrioides
120
-
 L-threo-phenylserine mutant D95L, pH 8.0, 30°C Caulobacter vibrioides
130
-
 L-threo-phenylserine mutant D95C, pH 8.0, 30°C Caulobacter vibrioides
1900
-
 L-threo-phenylserine wild-type, pH 8.0, 30°C Caulobacter vibrioides