Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
in complex with hydroxypyruvate, hanging drop vapor diffusion method, using 2.0 M ammonium sulfate and 0.1 M Bis-Tris, pH 6.5 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
181 | - |
N-acetyl-D-mannosamine | in 50 mM Tris-HCl, pH 7.8, at 37°C | Escherichia coli | |
199 | - |
L-arabinose | in 50 mM Tris-HCl, pH 7.8, at 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
sulfate | one sulfate ion is found adjacent to the side chain of Lys-165 in the active site and another one on the surface of RS-aldolase | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-deoxy-L-manno-octulosonate | - |
Escherichia coli | pyruvate + L-arabinose | - |
r | |
D-sialic acid | the RS-aldolase has a 5.8fold higher cleavage rate toward 3-deoxy-L-manno-octulosonate than toward D-sialic acid | Escherichia coli | N-acetyl-D-mannosamine + pyruvate | - |
r | |
N-acetyl-D-mannosamine + pyruvate | - |
Escherichia coli | D-sialic acid | - |
r | |
pyruvate + L-arabinose | - |
Escherichia coli | 3-deoxy-L-manno-octulosonate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
D-sialic acid aldolase mutant V251I | the mutant shows modified substrate specificity from D-sialic acid to 3-deoxy-L-manno-octulosonate | Escherichia coli |
L-3-deoxy-manno-2-octulosonic acid aldolase | formerly | Escherichia coli |
L-KDO aldolase | formerly | Escherichia coli |
RS-aldolase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
N-acetyl-D-mannosamine | in 50 mM Tris-HCl, pH 7.8, at 37°C | Escherichia coli | |
7.3 | - |
L-arabinose | in 50 mM Tris-HCl, pH 7.8, at 37°C | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0055 | - |
N-acetyl-D-mannosamine | in 50 mM Tris-HCl, pH 7.8, at 37°C | Escherichia coli | |
0.04 | - |
L-arabinose | in 50 mM Tris-HCl, pH 7.8, at 37°C | Escherichia coli |