BRENDA - Enzyme Database
show all sequences of 4.1.2.22

Phosphoketolases from Lactococcus lactis, Leuconostoc mesenteroides and Pseudomonas aeruginosa Dissimilar sequences, similar substrates but distinct enzymatic characteristics

Petrareanu, G.; Balasu, M.; Vacaru, A.; Munteanu, C.; Ionescu, A.; Matei, I.; Szedlacsek, S.; Appl. Microbiol. Biotechnol. 98, 7855-7867 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
cloned in a prokaryotic vector, and the encoded protein is expressed in Escherichia coli
Lactococcus lactis subsp. lactis
cloned in a prokaryotic vector, and the encoded protein is expressed in Escherichia coli
Leuconostoc mesenteroides subsp. mesenteroides
cloned in a prokaryotic vector, and the encoded protein is expressed in Escherichia coli; expressed in Escherichia coli BL21(DE3)RIL cells
Pseudomonas aeruginosa
expressed in Escherichia coli BL21(DE3)RIL cells
Lactococcus lactis
expressed in Escherichia coli BL21(DE3)RIL cells
Leuconostoc mesenteroides
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.035
-
D-xylulose 5-phosphate
at pH 6.5 and 37°C; pH 6.5, 37°C
Pseudomonas aeruginosa
1.76
-
D-fructose 6-phosphate
at pH 6.5 and 37°C; pH 6.5, 37°C
Pseudomonas aeruginosa
2.02
-
D-xylulose 5-phosphate
at pH 6.5 and 37°C
Leuconostoc mesenteroides
2.02
-
D-xylulose 5-phosphate
pH 6.5, 37°C
Leuconostoc mesenteroides subsp. mesenteroides
3.04
-
D-xylulose 5-phosphate
at pH 6.5 and 37°C
Lactococcus lactis
3.04
-
D-xylulose 5-phosphate
pH 6.5, 37°C
Lactococcus lactis subsp. lactis
9.89
-
D-fructose 6-phosphate
at pH 6.5 and 37°C
Lactococcus lactis
9.89
-
D-fructose 6-phosphate
pH 6.5, 37°C
Lactococcus lactis subsp. lactis
45.96
-
D-fructose 6-phosphate
at pH 6.5 and 37°C
Leuconostoc mesenteroides
45.96
-
D-fructose 6-phosphate
pH 6.5, 37°C
Leuconostoc mesenteroides subsp. mesenteroides
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-Fructose 6-phosphate + phosphate
Pseudomonas aeruginosa
-
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
?
D-Fructose 6-phosphate + phosphate
Lactococcus lactis
-
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
?
D-Fructose 6-phosphate + phosphate
Leuconostoc mesenteroides
-
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
?
D-Xylulose 5-phosphate + phosphate
Pseudomonas aeruginosa
preferred substrate
Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
-
?
D-Xylulose 5-phosphate + phosphate
Lactococcus lactis
preferred substrate
Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
-
?
D-Xylulose 5-phosphate + phosphate
Leuconostoc mesenteroides
preferred substrate
Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lactococcus lactis
-
-
-
Lactococcus lactis subsp. lactis
Q9CFH4
-
-
Lactococcus lactis subsp. lactis IL1403
Q9CFH4
-
-
Leuconostoc mesenteroides
-
-
-
Leuconostoc mesenteroides subsp. mesenteroides
A0A223XQ71
-
-
Leuconostoc mesenteroides subsp. mesenteroides J18
A0A223XQ71
-
-
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa
Q9HY13
-
-
Purification (Commentary)
Commentary
Organism
-
Lactococcus lactis
-
Lactococcus lactis subsp. lactis
-
Leuconostoc mesenteroides
-
Leuconostoc mesenteroides subsp. mesenteroides
;
Pseudomonas aeruginosa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-Fructose 6-phosphate + phosphate
-
746881
Pseudomonas aeruginosa
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
-
?
D-Fructose 6-phosphate + phosphate
-
746881
Lactococcus lactis
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
-
?
D-Fructose 6-phosphate + phosphate
-
746881
Leuconostoc mesenteroides
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
-
?
D-Fructose 6-phosphate + phosphate
the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate
746881
Lactococcus lactis subsp. lactis
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
-
?
D-Fructose 6-phosphate + phosphate
the bifunctional enzyme shows activity with fructose-6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for fructose-6-phosphate
746881
Pseudomonas aeruginosa
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
-
?
D-Fructose 6-phosphate + phosphate
the bifunctional enzyme shows activity with fructose-6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for fructose-6-phosphate
746881
Leuconostoc mesenteroides subsp. mesenteroides
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
-
?
D-Fructose 6-phosphate + phosphate
the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate
746881
Lactococcus lactis subsp. lactis IL1403
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
-
?
D-Fructose 6-phosphate + phosphate
the bifunctional enzyme shows activity with fructose-6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for fructose-6-phosphate
746881
Leuconostoc mesenteroides subsp. mesenteroides J18
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
-
?
D-Xylulose 5-phosphate + phosphate
preferred substrate
746881
Pseudomonas aeruginosa
Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
-
-
?
D-Xylulose 5-phosphate + phosphate
preferred substrate
746881
Lactococcus lactis
Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
-
-
?
D-Xylulose 5-phosphate + phosphate
preferred substrate
746881
Leuconostoc mesenteroides
Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
-
-
?
D-Xylulose 5-phosphate + phosphate
the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate
746881
Leuconostoc mesenteroides subsp. mesenteroides
Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
-
-
?
D-Xylulose 5-phosphate + phosphate
the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate
746881
Lactococcus lactis subsp. lactis
Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
-
-
?
D-Xylulose 5-phosphate + phosphate
the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose6-phosphate
746881
Pseudomonas aeruginosa
Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
-
-
?
D-Xylulose 5-phosphate + phosphate
the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate
746881
Lactococcus lactis subsp. lactis IL1403
Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
-
-
?
D-Xylulose 5-phosphate + phosphate
the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate
746881
Leuconostoc mesenteroides subsp. mesenteroides J18
Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 95700, SDS-PAGE
Lactococcus lactis
?
x * 91858, calculated from amino acid sequence; x * 94200, SDS-PAGE
Leuconostoc mesenteroides
?
x * 89200, SDS-PAGE
Pseudomonas aeruginosa
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Lactococcus lactis
37
-
-
Leuconostoc mesenteroides
37
-
-
Pseudomonas aeruginosa
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
40
the activity sharply decreases at 30 and 40°C to approximately 60% of the maximum specific activity observed at 37°C
Lactococcus lactis
30
40
the activity sharply decreases at 30 and 40°C to approximately 60% of the maximum specific activity observed at 37°C
Leuconostoc mesenteroides
30
40
the activity sharply decreases at 30 and 40°C to approximately 60% of the maximum specific activity observed at 37°C
Pseudomonas aeruginosa
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
13.05
-
D-fructose 6-phosphate
at pH 6.5 and 37°C
Pseudomonas aeruginosa
13.1
-
D-fructose 6-phosphate
pH 6.5, 37°C
Pseudomonas aeruginosa
13.18
-
D-xylulose 5-phosphate
at pH 6.5 and 37°C
Pseudomonas aeruginosa
13.2
-
D-xylulose 5-phosphate
pH 6.5, 37°C
Pseudomonas aeruginosa
44.2
-
D-fructose 6-phosphate
pH 6.5, 37°C
Leuconostoc mesenteroides subsp. mesenteroides
44.23
-
D-fructose 6-phosphate
at pH 6.5 and 37°C
Leuconostoc mesenteroides
117.76
-
D-fructose 6-phosphate
at pH 6.5 and 37°C
Lactococcus lactis
117.8
-
D-fructose 6-phosphate
pH 6.5, 37°C
Lactococcus lactis subsp. lactis
431.78
-
D-xylulose 5-phosphate
at pH 6.5 and 37°C
Leuconostoc mesenteroides
431.8
-
D-xylulose 5-phosphate
pH 6.5, 37°C
Leuconostoc mesenteroides subsp. mesenteroides
587.76
-
D-xylulose 5-phosphate
at pH 6.5 and 37°C
Lactococcus lactis
587.8
-
D-xylulose 5-phosphate
pH 6.5, 37°C
Lactococcus lactis subsp. lactis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Lactococcus lactis
7
-
-
Leuconostoc mesenteroides
7
-
-
Pseudomonas aeruginosa
Cofactor
Cofactor
Commentary
Organism
Structure
thiamine diphosphate
-
Lactococcus lactis
thiamine diphosphate
-
Lactococcus lactis subsp. lactis
thiamine diphosphate
-
Leuconostoc mesenteroides
thiamine diphosphate
-
Leuconostoc mesenteroides subsp. mesenteroides
thiamine diphosphate
;
Pseudomonas aeruginosa
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Leuconostoc mesenteroides
calculated from amino acid sequence
-
5
Cloned(Commentary) (protein specific)
Commentary
Organism
cloned in a prokaryotic vector, and the encoded protein is expressed in Escherichia coli
Lactococcus lactis subsp. lactis
cloned in a prokaryotic vector, and the encoded protein is expressed in Escherichia coli
Leuconostoc mesenteroides subsp. mesenteroides
cloned in a prokaryotic vector, and the encoded protein is expressed in Escherichia coli
Pseudomonas aeruginosa
expressed in Escherichia coli BL21(DE3)RIL cells
Lactococcus lactis
expressed in Escherichia coli BL21(DE3)RIL cells
Leuconostoc mesenteroides
expressed in Escherichia coli BL21(DE3)RIL cells
Pseudomonas aeruginosa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
thiamine diphosphate
-
Lactococcus lactis
thiamine diphosphate
-
Lactococcus lactis subsp. lactis
thiamine diphosphate
-
Leuconostoc mesenteroides
thiamine diphosphate
-
Leuconostoc mesenteroides subsp. mesenteroides
thiamine diphosphate
-
Pseudomonas aeruginosa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.035
-
D-xylulose 5-phosphate
pH 6.5, 37°C
Pseudomonas aeruginosa
0.035
-
D-xylulose 5-phosphate
at pH 6.5 and 37°C
Pseudomonas aeruginosa
1.76
-
D-fructose 6-phosphate
pH 6.5, 37°C
Pseudomonas aeruginosa
1.76
-
D-fructose 6-phosphate
at pH 6.5 and 37°C
Pseudomonas aeruginosa
2.02
-
D-xylulose 5-phosphate
at pH 6.5 and 37°C
Leuconostoc mesenteroides
2.02
-
D-xylulose 5-phosphate
pH 6.5, 37°C
Leuconostoc mesenteroides subsp. mesenteroides
3.04
-
D-xylulose 5-phosphate
at pH 6.5 and 37°C
Lactococcus lactis
3.04
-
D-xylulose 5-phosphate
pH 6.5, 37°C
Lactococcus lactis subsp. lactis
9.89
-
D-fructose 6-phosphate
at pH 6.5 and 37°C
Lactococcus lactis
9.89
-
D-fructose 6-phosphate
pH 6.5, 37°C
Lactococcus lactis subsp. lactis
45.96
-
D-fructose 6-phosphate
at pH 6.5 and 37°C
Leuconostoc mesenteroides
45.96
-
D-fructose 6-phosphate
pH 6.5, 37°C
Leuconostoc mesenteroides subsp. mesenteroides
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-Fructose 6-phosphate + phosphate
Pseudomonas aeruginosa
-
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
?
D-Fructose 6-phosphate + phosphate
Lactococcus lactis
-
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
?
D-Fructose 6-phosphate + phosphate
Leuconostoc mesenteroides
-
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
?
D-Xylulose 5-phosphate + phosphate
Pseudomonas aeruginosa
preferred substrate
Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
-
?
D-Xylulose 5-phosphate + phosphate
Lactococcus lactis
preferred substrate
Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
-
?
D-Xylulose 5-phosphate + phosphate
Leuconostoc mesenteroides
preferred substrate
Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Lactococcus lactis
-
Lactococcus lactis subsp. lactis
-
Leuconostoc mesenteroides
-
Leuconostoc mesenteroides subsp. mesenteroides
-
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-Fructose 6-phosphate + phosphate
-
746881
Pseudomonas aeruginosa
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
-
?
D-Fructose 6-phosphate + phosphate
-
746881
Lactococcus lactis
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
-
?
D-Fructose 6-phosphate + phosphate
-
746881
Leuconostoc mesenteroides
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
-
?
D-Fructose 6-phosphate + phosphate
the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate
746881
Lactococcus lactis subsp. lactis
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
-
?
D-Fructose 6-phosphate + phosphate
the bifunctional enzyme shows activity with fructose-6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for fructose-6-phosphate
746881
Pseudomonas aeruginosa
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
-
?
D-Fructose 6-phosphate + phosphate
the bifunctional enzyme shows activity with fructose-6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for fructose-6-phosphate
746881
Leuconostoc mesenteroides subsp. mesenteroides
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
-
?
D-Fructose 6-phosphate + phosphate
the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate
746881
Lactococcus lactis subsp. lactis IL1403
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
-
?
D-Fructose 6-phosphate + phosphate
the bifunctional enzyme shows activity with fructose-6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for fructose-6-phosphate
746881
Leuconostoc mesenteroides subsp. mesenteroides J18
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
-
?
D-Xylulose 5-phosphate + phosphate
preferred substrate
746881
Pseudomonas aeruginosa
Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
-
-
?
D-Xylulose 5-phosphate + phosphate
preferred substrate
746881
Lactococcus lactis
Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
-
-
?
D-Xylulose 5-phosphate + phosphate
preferred substrate
746881
Leuconostoc mesenteroides
Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
-
-
?
D-Xylulose 5-phosphate + phosphate
the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate
746881
Leuconostoc mesenteroides subsp. mesenteroides
Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
-
-
?
D-Xylulose 5-phosphate + phosphate
the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate
746881
Lactococcus lactis subsp. lactis
Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
-
-
?
D-Xylulose 5-phosphate + phosphate
the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose6-phosphate
746881
Pseudomonas aeruginosa
Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
-
-
?
D-Xylulose 5-phosphate + phosphate
the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate
746881
Lactococcus lactis subsp. lactis IL1403
Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
-
-
?
D-Xylulose 5-phosphate + phosphate
the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate
746881
Leuconostoc mesenteroides subsp. mesenteroides J18
Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 95700, SDS-PAGE
Lactococcus lactis
?
x * 91858, calculated from amino acid sequence; x * 94200, SDS-PAGE
Leuconostoc mesenteroides
?
x * 89200, SDS-PAGE
Pseudomonas aeruginosa
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Lactococcus lactis
37
-
-
Leuconostoc mesenteroides
37
-
-
Pseudomonas aeruginosa
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
40
the activity sharply decreases at 30 and 40°C to approximately 60% of the maximum specific activity observed at 37°C
Lactococcus lactis
30
40
the activity sharply decreases at 30 and 40°C to approximately 60% of the maximum specific activity observed at 37°C
Leuconostoc mesenteroides
30
40
the activity sharply decreases at 30 and 40°C to approximately 60% of the maximum specific activity observed at 37°C
Pseudomonas aeruginosa
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
13.05
-
D-fructose 6-phosphate
at pH 6.5 and 37°C
Pseudomonas aeruginosa
13.1
-
D-fructose 6-phosphate
pH 6.5, 37°C
Pseudomonas aeruginosa
13.18
-
D-xylulose 5-phosphate
at pH 6.5 and 37°C
Pseudomonas aeruginosa
13.2
-
D-xylulose 5-phosphate
pH 6.5, 37°C
Pseudomonas aeruginosa
44.2
-
D-fructose 6-phosphate
pH 6.5, 37°C
Leuconostoc mesenteroides subsp. mesenteroides
44.23
-
D-fructose 6-phosphate
at pH 6.5 and 37°C
Leuconostoc mesenteroides
117.76
-
D-fructose 6-phosphate
at pH 6.5 and 37°C
Lactococcus lactis
117.8
-
D-fructose 6-phosphate
pH 6.5, 37°C
Lactococcus lactis subsp. lactis
431.78
-
D-xylulose 5-phosphate
at pH 6.5 and 37°C
Leuconostoc mesenteroides
431.8
-
D-xylulose 5-phosphate
pH 6.5, 37°C
Leuconostoc mesenteroides subsp. mesenteroides
587.76
-
D-xylulose 5-phosphate
at pH 6.5 and 37°C
Lactococcus lactis
587.8
-
D-xylulose 5-phosphate
pH 6.5, 37°C
Lactococcus lactis subsp. lactis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Lactococcus lactis
7
-
-
Leuconostoc mesenteroides
7
-
-
Pseudomonas aeruginosa
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Leuconostoc mesenteroides
calculated from amino acid sequence
-
5
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.96
-
D-fructose 6-phosphate
at pH 6.5 and 37°C
Leuconostoc mesenteroides
0.96
-
D-fructose 6-phosphate
pH 6.5, 37°C
Leuconostoc mesenteroides subsp. mesenteroides
7.43
-
D-fructose 6-phosphate
at pH 6.5 and 37°C; pH 6.5, 37°C
Pseudomonas aeruginosa
11.9
-
D-fructose 6-phosphate
at pH 6.5 and 37°C
Lactococcus lactis
11.9
-
D-fructose 6-phosphate
pH 6.5, 37°C
Lactococcus lactis subsp. lactis
193.15
-
D-xylulose 5-phosphate
at pH 6.5 and 37°C
Lactococcus lactis
193.15
-
D-xylulose 5-phosphate
pH 6.5, 37°C
Lactococcus lactis subsp. lactis
213.43
-
D-xylulose 5-phosphate
at pH 6.5 and 37°C
Leuconostoc mesenteroides
213.43
-
D-xylulose 5-phosphate
pH 6.5, 37°C
Leuconostoc mesenteroides subsp. mesenteroides
378.62
-
D-xylulose 5-phosphate
at pH 6.5 and 37°C; pH 6.5, 37°C
Pseudomonas aeruginosa
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.96
-
D-fructose 6-phosphate
at pH 6.5 and 37°C
Leuconostoc mesenteroides
0.96
-
D-fructose 6-phosphate
pH 6.5, 37°C
Leuconostoc mesenteroides subsp. mesenteroides
7.43
-
D-fructose 6-phosphate
pH 6.5, 37°C
Pseudomonas aeruginosa
7.43
-
D-fructose 6-phosphate
at pH 6.5 and 37°C
Pseudomonas aeruginosa
11.9
-
D-fructose 6-phosphate
at pH 6.5 and 37°C
Lactococcus lactis
11.9
-
D-fructose 6-phosphate
pH 6.5, 37°C
Lactococcus lactis subsp. lactis
193.15
-
D-xylulose 5-phosphate
at pH 6.5 and 37°C
Lactococcus lactis
193.15
-
D-xylulose 5-phosphate
pH 6.5, 37°C
Lactococcus lactis subsp. lactis
213.43
-
D-xylulose 5-phosphate
at pH 6.5 and 37°C
Leuconostoc mesenteroides
213.43
-
D-xylulose 5-phosphate
pH 6.5, 37°C
Leuconostoc mesenteroides subsp. mesenteroides
378.62
-
D-xylulose 5-phosphate
pH 6.5, 37°C
Pseudomonas aeruginosa
378.62
-
D-xylulose 5-phosphate
at pH 6.5 and 37°C
Pseudomonas aeruginosa
Other publictions for EC 4.1.2.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747623
Pechar
Classification of culturable ...
Bifidobacterium boum, Bifidobacterium boum LMG 10736, Bifidobacterium boum ST6, Bifidobacterium choerinum, Bifidobacterium choerinum LMG 10510, Bifidobacterium pseudolongum subsp. globosum, Bifidobacterium pseudolongum subsp. globosum JCM 5820, Bifidobacterium pseudolongum subsp. globosum ST7, Bifidobacterium pseudolongum subsp. pseudolongum, Bifidobacterium pseudolongum subsp. pseudolongum JCM 1205, Bifidobacterium sp. ST1, Bifidobacterium sp. ST2, Bifidobacterium sp. ST3, Bifidobacterium sp. ST4, Bifidobacterium sp. ST5, Bifidobacterium thermacidophilum subsp. thermacidophilum, Bifidobacterium thermacidophilum subsp. thermacidophilum DSM 15837, Bifidobacterium thermophilum, Bifidobacterium thermophilum AS 1.2235, Bifidobacterium thermophilum subsp. porcinum, Bifidobacterium thermophilum subsp. porcinum P3-14, Bifidobacterium thermophilum subsp. porcinum ST8, Bifidobacterium thermophilum subsp. porcinum ST9
Curr. Microbiol.
74
1324-1331
2017
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23
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28
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23
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23
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23
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-
747991
Pechar
Bifidobacterium apri sp. nov. ...
Bifidobacterium apri, Bifidobacterium apri DSM 100238
Int. J. Syst. Evol. Microbiol.
67
2349-2356
2017
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2
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747992
Sechovcova
Alloscardovia venturai sp. no ...
Alloscardovia venturai, Alloscardovia venturai DSM 100237
Int. J. Syst. Evol. Microbiol.
67
2842-2847
2017
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2
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749102
Gupta
Novel molecular, structural a ...
Bifidobacterium animalis, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum, Bifidobacterium reuteri
PLoS ONE
12
e0172176
2017
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10
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5
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10
-
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746713
Bergman
Functional expression and eva ...
Bifidobacterium adolescentis, Bifidobacterium animalis subsp. lactis, Bifidobacterium breve, Clostridium acetobutylicum, Lactobacillus plantarum, Leuconostoc mesenteroides
AMB Express
6
115
2016
-
6
6
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-
-
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-
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7
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7
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7
7
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7
-
-
-
-
-
-
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-
-
-
6
7
-
-
-
747315
Moriyama
Characterization of three put ...
Nostoc sp. PCC 7120
Biosci. Biotechnol. Biochem.
79
767-774
2015
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2
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4
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1
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2
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1
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2
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-
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748094
Glenn
Allosteric regulation of Lact ...
Lactobacillus plantarum
J. Bacteriol.
197
1157-1163
2015
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-
1
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-
4
1
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2
-
4
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1
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2
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2
1
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1
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3
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1
1
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3
4
-
1
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2
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1
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-
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2
-
-
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2
1
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-
-
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2
2
748512
Taylor
Biolistic transformation of a ...
Cryptococcus neoformans
J. Vis. Exp.
97
e52666
2015
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-
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1
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4
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3
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1
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1
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3
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1
-
-
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-
-
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-
-
-
-
-
-
-
-
748753
Xiong
Phosphoketolase pathway contr ...
Synechocystis sp. DELTAglgC/xylAB
Nat. Plants
2
15187
2015
-
-
-
-
-
-
-
-
-
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-
1
-
1
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1
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1
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1
-
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-
-
-
-
-
-
-
-
-
-
-
727394
Glenn
Biochemical and kinetic charac ...
Cryptococcus neoformans
Eukaryot. Cell
13
657-663
2014
1
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-
-
-
-
3
-
-
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4
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1
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1
1
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1
3
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1
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-
-
-
746881
Petrareanu
Phosphoketolases from Lactoco ...
Lactococcus lactis, Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. lactis IL1403, Leuconostoc mesenteroides, Leuconostoc mesenteroides subsp. mesenteroides, Leuconostoc mesenteroides subsp. mesenteroides J18, Pseudomonas aeruginosa
Appl. Microbiol. Biotechnol.
98
7855-7867
2014
-
-
5
-
-
-
-
10
-
-
-
6
-
8
-
-
5
-
-
-
-
-
16
3
3
3
-
12
3
-
-
5
-
1
-
-
-
6
6
-
-
-
-
-
-
12
-
-
-
6
-
-
-
6
-
-
-
-
16
3
3
3
-
12
3
-
-
1
-
-
-
-
10
12
747246
Tittmann
Sweet siblings with different ...
Bifidobacterium breve
Bioorg. Chem.
57
263-280
2014
-
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2
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1
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2
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1
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1
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2
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2
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-
-
-
-
-
-
728652
Bolado-Martinez
Fructose 6-phosphate phosphoke ...
Lactobacillus sp.
Prikl. Biokhim. Mikrobiol.
48
494-500
2012
-
-
-
-
-
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8
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1
1
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-
-
713918
Fleige
Establishment of an alternativ ...
Cupriavidus necator
Appl. Microbiol. Biotechnol.
91
769-776
2011
-
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1
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1
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4
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2
1
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1
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1
1
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1
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2
1
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-
-
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-
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-
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-
-
-
-
704232
Cleusix
Comparative study of a new qua ...
Bifidobacterium animalis subsp. lactis, Bifidobacterium longum
J. Appl. Microbiol.
108
181-193
2010
-
-
-
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2
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5
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2
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2
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-
-
-
-
-
-
-
-
-
713652
Suzuki
Overexpression, crystallizatio ...
Bifidobacterium breve 203, Bifidobacterium breve
Acta Crystallogr. Sect. F
66
941-943
2010
-
-
1
1
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2
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1
5
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6
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1
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4
1
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2
1
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1
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1
1
1
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2
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1
5
-
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1
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-
-
4
1
-
-
-
2
1
-
-
-
-
-
-
-
-
-
715026
Takahashi
Crystal structure of Bifidobac ...
Bifidobacterium longum, Bifidobacterium longum JCM 1217
FEBS Lett.
584
3855-3861
2010
-
-
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1
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1
1
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6
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1
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4
1
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1
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1
1
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1
1
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1
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4
1
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-
-
-
-
-
-
715551
Suzuki
Crystal structures of phosphok ...
Bifidobacterium breve
J. Biol. Chem.
285
34279-34287
2010
-
-
1
1
17
-
-
14
-
1
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2
-
3
-
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-
-
-
-
-
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2
1
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7
-
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1
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1
1
1
17
-
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14
-
1
-
2
-
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-
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-
-
-
2
1
-
-
-
7
-
-
-
-
-
-
-
-
-
-
701819
Martin
Isolation of bifidobacteria fr ...
Bifidobacterium adolescentis, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium dentium, Bifidobacterium longum
Appl. Environ. Microbiol.
75
965-969
2009
-
-
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-
-
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10
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5
-
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-
-
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-
-
-
-
-
-
-
-
-
701841
Roopashri
Molecular characterization of ...
Bifidobacterium adolescentis, Bifidobacterium adolescentis MTCC 5423
Appl. Microbiol. Biotechnol.
83
1115-1126
2009
-
-
-
-
-
-
-
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-
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2
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10
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2
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2
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-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704102
Watanabe
Bifidobacterium mongoliense sp ...
Bifidobacterium mongoliense
Int. J. Syst. Evol. Microbiol.
59
1535-1540
2009
-
-
-
-
-
-
-
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1
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5
-
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1
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1
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1
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-
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-
704103
Killer
Bifidobacterium bombi sp. nov. ...
Bifidobacterium bombi, Bifidobacterium bombi BluCI/TP / DSM 19703 / ATCC BAA-1567
Int. J. Syst. Evol. Microbiol.
59
2020-2024
2009
-
-
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10
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681361
Jeong
Cloning and characterization o ...
Lactobacillus paraplantarum, Lactobacillus paraplantarum C7
J. Microbiol. Biotechnol.
17
822-829
2007
-
-
1
-
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1
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1
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2
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1
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2
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2
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1
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1
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1
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1
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1
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1
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2
-
2
-
1
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1
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-
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-
664428
Lee
Cloning and characterization o ...
Leuconostoc mesenteroides, Leuconostoc mesenteroides C7
Biotechnol. Lett.
27
853-858
2005
-
-
1
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2
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2
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2
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-
664992
Yin
The gene encoding xylulose-5-p ...
Bifidobacterium sp.
FEMS Microbiol. Lett.
246
251-257
2005
-
1
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3
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-
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-
-
-
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-
664983
Sanchez
Acquired resistance to bile in ...
Bifidobacterium sp.
FEMS Microbiol. Lett.
235
35-41
2004
-
-
-
-
-
-
-
-
1
-
1
1
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3
-
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1
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1
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-
-
-
-
-
-
-
-
-
651682
Meile
Characterization of the D-xylu ...
Bifidobacterium animalis subsp. lactis
J. Bacteriol.
183
2929-2936
2001
-
-
1
-
-
-
-
2
-
-
2
-
-
1
-
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1
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1
-
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-
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-
-
-
653088
Fandi
Purification and N-terminal am ...
Bifidobacterium longum, Bifidobacterium longum BB536
Lett. Appl. Microbiol.
32
235-239
2001
-
-
-
-
-
-
-
-
-
-
3
-
-
4
-
-
1
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-
5098
Gavini
-
Detection of fructose-6-phosph ...
Bifidobacterium bifidum, Bifidobacterium sp., Gardnerella vaginalis, no activity in Actinomyces turicensis-like bacteria
Anaerobe
2
191-193
1996
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
4
-
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-
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4
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5097
Whitworth
-
Phosphoketolase in Rhodotorula ...
Candida sp. (in: Saccharomycetales), Candida sp. (in: Saccharomycetales) 107, Candida tropicalis, Rhodotorula glutinis, Rhodotorula graminis, Saccharomyces pastorianus
J. Gen. Microbiol.
102
397-401
1977
1
-
-
-
-
-
-
-
-
1
-
-
-
6
-
-
1
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6
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-
-
6
-
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-
-
-
-
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-
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-
-
-
-
-
5096
Sgorbath
Purification and properties of ...
Bifidobacterium dentium, Bifidobacterium pseudolongum subsp. globosum
Antonie van Leeuwenhoek
42
49-57
1976
-
-
-
-
-
-
8
2
-
4
2
-
-
8
-
-
2
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2
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11
-
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-
6
-
2
2
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8
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2
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2
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2
-
11
-
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6
-
2
2
-
-
-
-
-
-
-
-
5095
Schramm
Phosphorolytic cleavage of fru ...
Komagataeibacter xylinus
J. Biol. Chem.
233
1283-1288
1958
1
-
-
-
-
-
-
2
-
1
-
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2
-
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