Literature summary for 4.1.2.22 extracted from

Suzuki, R.; Katayama, T.; Kim, B.J.; Wakagi, T.; Shoun, H.; Ashida, H.; Yamamoto, K.; Fushinobu, S.
Crystal structures of phosphoketolase: thiamine diphosphate-dependent dehydration mechanism (2010), J. Biol. Chem., 285, 34279-34287.

Search for more literature for 4.1.2.22

Cloned(Commentary)

Cloned (Comment)	Organism
selenomethionine-labeled enzyme is expressed in Escherichia coli B834 (DE3) cells	Bifidobacterium breve

Crystallization (Commentary)

Crystallization (Comment)	Organism
cryocrystallography technique, using 24% (w/v) PEG 6000, 0.1 M bicine buffer, pH 9.0	Bifidobacterium breve

Protein Variants

Protein Variants	Comment	Organism
E479A	inactive	Bifidobacterium breve
H142A	the mutant shows reduced kcat with D-fructose 6-phosphate compared to the wild type enzyme	Bifidobacterium breve
H142N	the mutant shows reduced kcat with D-fructose 6-phosphate compared to the wild type enzyme	Bifidobacterium breve
H320A	inactive	Bifidobacterium breve
H320N	inactive	Bifidobacterium breve
H548A	the mutant shows reduced kcat with D-fructose 6-phosphate compared to the wild type enzyme	Bifidobacterium breve
H553A	inactive	Bifidobacterium breve
H553N	inactive	Bifidobacterium breve
H64A	inactive	Bifidobacterium breve
H64N	inactive	Bifidobacterium breve
H97A	inactive	Bifidobacterium breve
H97N	inactive	Bifidobacterium breve
K605A	inactive	Bifidobacterium breve
N549A	inactive	Bifidobacterium breve
Q321A	the mutant shows reduced kcat with D-fructose 6-phosphate compared to the wild type enzyme	Bifidobacterium breve
S440A	the mutant shows reduced kcat with D-fructose 6-phosphate compared to the wild type enzyme	Bifidobacterium breve
Y501F	the mutant shows reduced kcat with D-fructose 6-phosphate compared to the wild type enzyme	Bifidobacterium breve

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.52	-	phosphate	mutant enzyme H548A, pH and temperature not specified in the publication	Bifidobacterium breve
0.57	-	phosphate	mutant enzyme S440A, pH and temperature not specified in the publication	Bifidobacterium breve
1.2	-	phosphate	wild type enzyme, pH and temperature not specified in the publication	Bifidobacterium breve
1.4	-	D-fructose 6-phosphate	mutant enzyme Y501F, pH and temperature not specified in the publication	Bifidobacterium breve
1.6	-	phosphate	mutant enzyme Q321A, pH and temperature not specified in the publication	Bifidobacterium breve
1.7	-	phosphate	mutant enzyme H142A, pH and temperature not specified in the publication	Bifidobacterium breve
2.3	-	D-fructose 6-phosphate	mutant enzyme H548A, pH and temperature not specified in the publication	Bifidobacterium breve
2.6	-	phosphate	mutant enzyme H142N, pH and temperature not specified in the publication	Bifidobacterium breve
4.4	-	D-fructose 6-phosphate	mutant enzyme Q321A, pH and temperature not specified in the publication	Bifidobacterium breve
7.2	-	D-fructose 6-phosphate	mutant enzyme H142N, pH and temperature not specified in the publication	Bifidobacterium breve
7.4	-	D-fructose 6-phosphate	mutant enzyme H142A, pH and temperature not specified in the publication	Bifidobacterium breve
9.7	-	D-fructose 6-phosphate	wild type enzyme, pH and temperature not specified in the publication	Bifidobacterium breve
25.5	-	phosphate	mutant enzyme Y501F, pH and temperature not specified in the publication	Bifidobacterium breve
53.5	-	D-fructose 6-phosphate	mutant enzyme S440A, pH and temperature not specified in the publication	Bifidobacterium breve

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Bifidobacterium breve

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-Fructose 6-phosphate + phosphate	Bifidobacterium breve	-	Acetyl phosphate + D-erythrose 4-phosphate + H2O	-	?
D-xylulose 5-phosphate + phosphate	Bifidobacterium breve	-	acetyl phosphate + D-glyceraldehyde 3-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Bifidobacterium breve	D6PAH1	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Fructose 6-phosphate + phosphate	-	Bifidobacterium breve	Acetyl phosphate + D-erythrose 4-phosphate + H2O	-	?
D-xylulose 5-phosphate + phosphate	-	Bifidobacterium breve	acetyl phosphate + D-glyceraldehyde 3-phosphate	-	?

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Bifidobacterium breve

Synonyms

Synonyms	Comment	Organism
XFPK	-	Bifidobacterium breve
xylulose 5-phosphate/fructose 6-phosphate phosphoketolase	-	Bifidobacterium breve

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.2	-	D-fructose 6-phosphate	mutant enzyme H142A, pH and temperature not specified in the publication	Bifidobacterium breve
0.65	-	D-fructose 6-phosphate	mutant enzyme H142N, pH and temperature not specified in the publication	Bifidobacterium breve
0.99	-	D-fructose 6-phosphate	mutant enzyme Q321A, pH and temperature not specified in the publication	Bifidobacterium breve
1.67	-	D-fructose 6-phosphate	mutant enzyme H548A, pH and temperature not specified in the publication	Bifidobacterium breve
3.28	-	D-fructose 6-phosphate	mutant enzyme Y501F, pH and temperature not specified in the publication	Bifidobacterium breve
5.83	-	D-fructose 6-phosphate	mutant enzyme S440A, pH and temperature not specified in the publication	Bifidobacterium breve
25.67	-	D-fructose 6-phosphate	wild type enzyme, pH and temperature not specified in the publication	Bifidobacterium breve

Cofactor

Cofactor	Comment	Organism	Structure
thiamine diphosphate	dependent on	Bifidobacterium breve

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Release 2023.1 (January 2023)