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Literature summary for 4.1.2.22 extracted from

  • Takahashi, K.; Tagami, U.; Shimba, N.; Kashiwagi, T.; Ishikawa, K.; Suzuki, E.
    Crystal structure of Bifidobacterium longum phosphoketolase; key enzyme for glucose metabolism in Bifidobacterium (2010), FEBS Lett., 584, 3855-3861.
    View publication on PubMed
Search for more literature for 4.1.2.22

Crystallization (Commentary)

Crystallization (Comment) Organism
using 10 mg/ml thiamine diphosphate, 4% (v/v) Tacsimate pH 5.0, 12% (w/v) polyethylene glycol 3350 for the native enzyme. Se-Met substituted protein crystals are grown with 17% (w/v) polyethylene glycol 3350, 0.1 M MES (pH6.6), 0.2 M sodium thiocyanate in the absence of thiamine diphosphate Bifidobacterium longum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ contains Ca2+ bound to the active site Bifidobacterium longum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
92500
-
 2 * 92500, SDS-PAGE Bifidobacterium longum

Organism

Organism UniProt Comment Textmining
Bifidobacterium longum
-
-
-
Bifidobacterium longum JCM 1217
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography, MonoQ column chromatography, and Superdex 200 gel filtration Bifidobacterium longum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Fructose 6-phosphate + phosphate
-
 Bifidobacterium longum Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
 ?
D-Fructose 6-phosphate + phosphate
-
 Bifidobacterium longum JCM 1217 Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
 ?
D-xylulose 5-phosphate + phosphate
-
 Bifidobacterium longum acetyl phosphate + D-glyceraldehyde 3-phosphate
-
 ?
D-xylulose 5-phosphate + phosphate
-
 Bifidobacterium longum JCM 1217 acetyl phosphate + D-glyceraldehyde 3-phosphate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 92500, SDS-PAGE Bifidobacterium longum

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate dependent on Bifidobacterium longum