BRENDA - Enzyme Database show
show all sequences of 4.1.2.20

The nonphosphorylative Entner-Doudoroff pathway in the thermoacidophilic euryarchaeon Picrophilus torridus involves a novel 2-keto-3-deoxygluconate-specific aldolase

Reher, M.; Fuhrer, T.; Bott, M.; Schoenheit, P.; J. Bacteriol. 192, 964-974 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21 CodonPlus (DE3)-RIL cells
Picrophilus torridus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.3
-
2-dehydro-3-deoxy-D-gluconate
at 60°C in 50 mM sodium phosphate buffer (pH 6.2)
Picrophilus torridus
8
-
2-dehydro-3-deoxy-6-phospho-D-gluconate
at 60°C in 50 mM sodium phosphate buffer (pH 6.2)
Picrophilus torridus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
4 * 32000, SDS-PAGE
Picrophilus torridus
120000
-
gel filtration
Picrophilus torridus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Picrophilus torridus
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, UnoQ5 column chromatography, and Superdex 200 gel filtration
Picrophilus torridus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:glucose-grown cell
-
Picrophilus torridus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.14
-
crude extract, at 60°C in 50 mM sodium phosphate buffer (pH 6.2)
Picrophilus torridus
48
-
after 343fold purification, at 60°C in 50 mM sodium phosphate buffer (pH 6.2)
Picrophilus torridus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-dehydro-3-deoxy-6-phospho-D-gluconate
-
704334
Picrophilus torridus
pyruvate + glyceraldehyde-3-phosphate
-
-
-
?
2-dehydro-3-deoxy-D-gluconate
the enzyme is highly specific for 2-dehydro-3-deoxy-D-gluconate with up to 2000fold higher catalytic efficiency compared to 2-dehydro-3-deoxy-6-phosphogluconate
704334
Picrophilus torridus
pyruvate + glyceraldehyde
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 32000, SDS-PAGE
Picrophilus torridus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
65
-
-
Picrophilus torridus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
90
at 70°C, the enzyme does not lose activity upon incubation for 2 h. The half-lives of the enzyme at 80°C and 90°C are 20 min and 15 min, respectively
Picrophilus torridus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.34
-
2-dehydro-3-deoxy-6-phospho-D-gluconate
at 60°C in 50 mM sodium phosphate buffer (pH 6.2)
Picrophilus torridus
26.7
-
2-dehydro-3-deoxy-D-gluconate
at 60°C in 50 mM sodium phosphate buffer (pH 6.2)
Picrophilus torridus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
-
Picrophilus torridus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.5
7.5
-
Picrophilus torridus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21 CodonPlus (DE3)-RIL cells
Picrophilus torridus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.3
-
2-dehydro-3-deoxy-D-gluconate
at 60°C in 50 mM sodium phosphate buffer (pH 6.2)
Picrophilus torridus
8
-
2-dehydro-3-deoxy-6-phospho-D-gluconate
at 60°C in 50 mM sodium phosphate buffer (pH 6.2)
Picrophilus torridus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
4 * 32000, SDS-PAGE
Picrophilus torridus
120000
-
gel filtration
Picrophilus torridus
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, UnoQ5 column chromatography, and Superdex 200 gel filtration
Picrophilus torridus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:glucose-grown cell
-
Picrophilus torridus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.14
-
crude extract, at 60°C in 50 mM sodium phosphate buffer (pH 6.2)
Picrophilus torridus
48
-
after 343fold purification, at 60°C in 50 mM sodium phosphate buffer (pH 6.2)
Picrophilus torridus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-dehydro-3-deoxy-6-phospho-D-gluconate
-
704334
Picrophilus torridus
pyruvate + glyceraldehyde-3-phosphate
-
-
-
?
2-dehydro-3-deoxy-D-gluconate
the enzyme is highly specific for 2-dehydro-3-deoxy-D-gluconate with up to 2000fold higher catalytic efficiency compared to 2-dehydro-3-deoxy-6-phosphogluconate
704334
Picrophilus torridus
pyruvate + glyceraldehyde
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 32000, SDS-PAGE
Picrophilus torridus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
65
-
-
Picrophilus torridus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
90
at 70°C, the enzyme does not lose activity upon incubation for 2 h. The half-lives of the enzyme at 80°C and 90°C are 20 min and 15 min, respectively
Picrophilus torridus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.34
-
2-dehydro-3-deoxy-6-phospho-D-gluconate
at 60°C in 50 mM sodium phosphate buffer (pH 6.2)
Picrophilus torridus
26.7
-
2-dehydro-3-deoxy-D-gluconate
at 60°C in 50 mM sodium phosphate buffer (pH 6.2)
Picrophilus torridus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
-
Picrophilus torridus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.5
7.5
-
Picrophilus torridus
General Information
General Information
Commentary
Organism
metabolism
Picrophilus torridus degrades glucose via a strictly nonphosphorylative Entner-Doudoroff pathway with a 2-keto-3-deoxygluconate-specific aldolase
Picrophilus torridus
General Information (protein specific)
General Information
Commentary
Organism
metabolism
Picrophilus torridus degrades glucose via a strictly nonphosphorylative Entner-Doudoroff pathway with a 2-keto-3-deoxygluconate-specific aldolase
Picrophilus torridus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.04
-
2-dehydro-3-deoxy-6-phospho-D-gluconate
at 60°C in 50 mM sodium phosphate buffer (pH 6.2)
Picrophilus torridus
89
-
2-dehydro-3-deoxy-D-gluconate
at 60°C in 50 mM sodium phosphate buffer (pH 6.2)
Picrophilus torridus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.04
-
2-dehydro-3-deoxy-6-phospho-D-gluconate
at 60°C in 50 mM sodium phosphate buffer (pH 6.2)
Picrophilus torridus
89
-
2-dehydro-3-deoxy-D-gluconate
at 60°C in 50 mM sodium phosphate buffer (pH 6.2)
Picrophilus torridus
Other publictions for EC 4.1.2.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
716878
Bhaskar
Identification of biochemical ...
Escherichia coli
Proteins
79
1132-1142
2011
-
-
1
1
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
704334
Reher
The nonphosphorylative Entner- ...
Picrophilus torridus
J. Bacteriol.
192
964-974
2010
-
-
1
-
-
-
-
2
-
-
2
-
-
3
-
-
1
-
-
1
2
-
2
1
1
-
1
2
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
1
-
1
2
-
2
1
1
-
1
2
1
1
-
-
-
1
1
-
2
2
691007
Rea
Crystal structure and function ...
Escherichia coli
Biochemistry
47
9955-9965
2008
-
-
1
1
-
-
-
7
-
2
-
-
-
2
-
-
1
-
-
-
-
-
6
-
-
-
-
7
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
7
-
2
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
7
1
-
-
-
-
-
-
-
-
-
677338
Li
Purification, crystallization ...
Leptospira interrogans
Acta Crystallogr. Sect. F
62
1269-1270
2006
-
-
1
1
-
-
-
-
-
2
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
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-
-
-
-
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-
1
-
1
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-
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2
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-
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-
-
1
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-
-
-
1
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
650974
Izard
Crystal structures of the meta ...
Escherichia coli
EMBO J.
19
3849-3856
2000
-
-
-
1
-
-
-
-
-
3
-
-
-
2
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649100
Blackwell
-
Rhombohedral crystals of 2-deh ...
Escherichia coli
Acta Crystallogr. Sect. D
55
1368-1369
1999
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
5083
Hubbard
Evolution of enzymatic activit ...
Escherichia coli
Biochemistry
37
14369-14375
1998
-
-
1
-
-
-
-
1
-
-
-
2
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
5082
Blumenthal
Specific enzymatic assay for D ...
Escherichia coli
Anal. Biochem.
185
286-293
1990
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5081
Marsh
An enzymatic determination of ...
Escherichia coli, Escherichia coli NCTC 10418
Anal. Biochem.
145
266-272
1985
-
1
-
-
-
-
-
-
-
-
-
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-
2
-
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-
-
-
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2
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1
-
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-
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-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5080
Jeffcoat
Bacterial hydrolases and aldol ...
Klebsiella aerogenes
Nature New Biol.
241
186-187
1973
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
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1
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1
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-
1
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-
-
-
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-
-
-
-
-
-
-
-
4971
Wood
-
2-Keto-3-deoxy-6-phosphoglucon ...
Escherichia coli, no activity in Bacillus subtilis
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
7
281-302
1972
-
-
-
-
-
-
-
-
-
5
-
1
-
2
-
-
-
-
-
-
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-
8
-
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-
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5
-
1
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-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5078
Fish
-
2-keto-3-deoxy-D-Glucarate ald ...
Escherichia coli, Escherichia coli CR63MA
Methods Enzymol.
9
529-534
1966
-
-
-
-
-
-
4
1
-
5
-
-
-
4
-
-
1
-
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-
1
-
6
-
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-
1
-
1
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4
-
1
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5
-
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1
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1
-
6
-
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1
-
1
-
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