BRENDA - Enzyme Database show
show all sequences of 4.1.2.20

Crystal structure and functional assignment of YfaU, a metal ion dependent class II aldolase from Escherichia coli K12

Rea, D.; Hovington, R.; Rakus, J.F.; Gerlt, J.A.; Fueloep, V.; Bugg, T.D.; Roper, D.I.; Biochemistry 47, 9955-9965 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
into the plasmid pT7 for expression in Escherichia coli B834DE3 pLacIRARE2 cells
Escherichia coli
Crystallization (Commentary)
Crystallization
Organism
the crystal structure of apo-YfaU is determined to 1.39 A resolution and the structure of a Mg2+-pyruvate product complex to 1.93 A resolution
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
4-hydroxy-2-ketohexanoic acid
data for Ni2+-activated YfaU
Escherichia coli
0.078
-
2-dehydro-3-deoxy-L-rhamnonic acid
data for Mg2+-activated YfaU
Escherichia coli
0.1
-
4-hydroxy-2-ketoheptane-1,7-dioate
data for Ni2+-activated YfaU
Escherichia coli
0.1
-
4-hydroxy-2-ketopentanoic acid
data for Ni2+-activated YfaU
Escherichia coli
0.14
-
2-keto-3-deoxy-L-mannonate
data for Mg2+-activated YfaU
Escherichia coli
0.15
-
4-hydroxy-2-ketoheptane-1,7-dioate
data for Mg2+-activated YfaU
Escherichia coli
0.8
-
2-keto-3-deoxy-L-lyxonate
data for Mg2+-activated YfaU
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Escherichia coli
Ni2+
-
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P23522
-
-
Purification (Commentary)
Commentary
Organism
using metal affinity and size exclusion chromatography
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-dehydro-3-deoxy-L-rhamnonate
-
691007
Escherichia coli
?
-
-
-
?
2-keto-3-deoxy-L-lyxonate
-
691007
Escherichia coli
?
-
-
-
?
2-keto-3-deoxy-L-mannonate
-
691007
Escherichia coli
?
-
-
-
?
4-hydroxy-2-ketoheptane-1,7-dioate
-
691007
Escherichia coli
pyruvate + succinic semialdehyde
-
-
-
?
4-hydroxy-2-ketohexanoic acid
-
691007
Escherichia coli
?
-
-
-
?
4-hydroxy-2-ketopentanoic acid
-
691007
Escherichia coli
?
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.3
-
2-keto-3-deoxy-L-lyxonate
data for Mg2+-activated YfaU
Escherichia coli
0.3
-
2-keto-3-deoxy-L-mannonate
data for Mg2+-activated YfaU
Escherichia coli
0.4
-
2-dehydro-3-deoxy-L-rhamnonic acid
data for Mg2+-activated YfaU
Escherichia coli
0.54
-
4-hydroxy-2-ketoheptane-1,7-dioate
data for Mg2+-activated YfaU
Escherichia coli
299
-
4-hydroxy-2-ketoheptane-1,7-dioate
data for Ni2+-activated YfaU
Escherichia coli
396
-
4-hydroxy-2-ketopentanoic acid
data for Ni2+-activated YfaU
Escherichia coli
447
-
4-hydroxy-2-ketohexanoic acid
data for Ni2+-activated YfaU
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
activity assay
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
into the plasmid pT7 for expression in Escherichia coli B834DE3 pLacIRARE2 cells
Escherichia coli
Crystallization (Commentary) (protein specific)
Crystallization
Organism
the crystal structure of apo-YfaU is determined to 1.39 A resolution and the structure of a Mg2+-pyruvate product complex to 1.93 A resolution
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
4-hydroxy-2-ketohexanoic acid
data for Ni2+-activated YfaU
Escherichia coli
0.078
-
2-dehydro-3-deoxy-L-rhamnonic acid
data for Mg2+-activated YfaU
Escherichia coli
0.1
-
4-hydroxy-2-ketoheptane-1,7-dioate
data for Ni2+-activated YfaU
Escherichia coli
0.1
-
4-hydroxy-2-ketopentanoic acid
data for Ni2+-activated YfaU
Escherichia coli
0.14
-
2-keto-3-deoxy-L-mannonate
data for Mg2+-activated YfaU
Escherichia coli
0.15
-
4-hydroxy-2-ketoheptane-1,7-dioate
data for Mg2+-activated YfaU
Escherichia coli
0.8
-
2-keto-3-deoxy-L-lyxonate
data for Mg2+-activated YfaU
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Escherichia coli
Ni2+
-
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
using metal affinity and size exclusion chromatography
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-dehydro-3-deoxy-L-rhamnonate
-
691007
Escherichia coli
?
-
-
-
?
2-keto-3-deoxy-L-lyxonate
-
691007
Escherichia coli
?
-
-
-
?
2-keto-3-deoxy-L-mannonate
-
691007
Escherichia coli
?
-
-
-
?
4-hydroxy-2-ketoheptane-1,7-dioate
-
691007
Escherichia coli
pyruvate + succinic semialdehyde
-
-
-
?
4-hydroxy-2-ketohexanoic acid
-
691007
Escherichia coli
?
-
-
-
?
4-hydroxy-2-ketopentanoic acid
-
691007
Escherichia coli
?
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.3
-
2-keto-3-deoxy-L-lyxonate
data for Mg2+-activated YfaU
Escherichia coli
0.3
-
2-keto-3-deoxy-L-mannonate
data for Mg2+-activated YfaU
Escherichia coli
0.4
-
2-dehydro-3-deoxy-L-rhamnonic acid
data for Mg2+-activated YfaU
Escherichia coli
0.54
-
4-hydroxy-2-ketoheptane-1,7-dioate
data for Mg2+-activated YfaU
Escherichia coli
299
-
4-hydroxy-2-ketoheptane-1,7-dioate
data for Ni2+-activated YfaU
Escherichia coli
396
-
4-hydroxy-2-ketopentanoic acid
data for Ni2+-activated YfaU
Escherichia coli
447
-
4-hydroxy-2-ketohexanoic acid
data for Ni2+-activated YfaU
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
activity assay
Escherichia coli
Other publictions for EC 4.1.2.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
716878
Bhaskar
Identification of biochemical ...
Escherichia coli
Proteins
79
1132-1142
2011
-
-
1
1
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
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-
1
-
1
-
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1
-
-
-
-
2
1
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-
-
-
-
-
-
1
1
-
-
-
704334
Reher
The nonphosphorylative Entner- ...
Picrophilus torridus
J. Bacteriol.
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-
-
1
-
-
-
-
2
-
-
2
-
-
3
-
-
1
-
-
1
2
-
2
1
1
-
1
2
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
1
-
1
2
-
2
1
1
-
1
2
1
1
-
-
-
1
1
-
2
2
691007
Rea
Crystal structure and function ...
Escherichia coli
Biochemistry
47
9955-9965
2008
-
-
1
1
-
-
-
7
-
2
-
-
-
2
-
-
1
-
-
-
-
-
6
-
-
-
-
7
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
7
-
2
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
7
1
-
-
-
-
-
-
-
-
-
677338
Li
Purification, crystallization ...
Leptospira interrogans
Acta Crystallogr. Sect. F
62
1269-1270
2006
-
-
1
1
-
-
-
-
-
2
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
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-
-
-
-
-
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1
-
1
-
-
-
-
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2
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-
1
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-
-
1
-
-
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650974
Izard
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Escherichia coli
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-
-
-
1
-
-
-
-
-
3
-
-
-
2
-
-
1
1
-
-
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-
1
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1
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3
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1
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-
1
-
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-
-
-
-
-
-
-
-
-
-
649100
Blackwell
-
Rhombohedral crystals of 2-deh ...
Escherichia coli
Acta Crystallogr. Sect. D
55
1368-1369
1999
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
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-
1
-
1
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-
1
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-
-
1
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-
1
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-
1
-
1
-
-
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-
1
-
-
-
-
-
-
-
-
-
5083
Hubbard
Evolution of enzymatic activit ...
Escherichia coli
Biochemistry
37
14369-14375
1998
-
-
1
-
-
-
-
1
-
-
-
2
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
5082
Blumenthal
Specific enzymatic assay for D ...
Escherichia coli
Anal. Biochem.
185
286-293
1990
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
5081
Marsh
An enzymatic determination of ...
Escherichia coli, Escherichia coli NCTC 10418
Anal. Biochem.
145
266-272
1985
-
1
-
-
-
-
-
-
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2
-
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2
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1
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-
2
-
-
-
-
-
-
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5080
Jeffcoat
Bacterial hydrolases and aldol ...
Klebsiella aerogenes
Nature New Biol.
241
186-187
1973
-
-
-
-
-
-
-
-
-
1
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1
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1
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1
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-
4971
Wood
-
2-Keto-3-deoxy-6-phosphoglucon ...
Escherichia coli, no activity in Bacillus subtilis
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
7
281-302
1972
-
-
-
-
-
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5
-
1
-
2
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8
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5
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1
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8
-
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5078
Fish
-
2-keto-3-deoxy-D-Glucarate ald ...
Escherichia coli, Escherichia coli CR63MA
Methods Enzymol.
9
529-534
1966
-
-
-
-
-
-
4
1
-
5
-
-
-
4
-
-
1
-
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-
1
-
6
-
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-
1
-
1
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4
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1
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5
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1
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1
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6
-
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1
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1
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