BRENDA - Enzyme Database
show all sequences of 4.1.2.20

Identification of biochemical and putative biological role of a xenolog from Escherichia coli using structural analysis

Bhaskar, V.; Kumar, M.; Manicka, S.; Tripathi, S.; Venkatraman, A.; Krishnaswamy, S.; Proteins 79, 1132-1142 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21 (DE3) cells
Escherichia coli
Crystallization (Commentary)
Crystallization
Organism
micro batch method, cocrystallization of YagE with 2-keto-3-deoxy galactonate and with pyruvate and glyceraldehyde in 100 mM HEPES pH 6.5, 200 mM MgCl2, 15% (w/v) PEG 3350, 50 mM pyruvate, and 50 mM glyceraldehyde
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P75682
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-dehydro-3-deoxy-D-galactonate
-
716878
Escherichia coli
?
-
-
-
?
pyruvate + glyceraldehyde
-
716878
Escherichia coli
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
x-ray crystallography
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21 (DE3) cells
Escherichia coli
Crystallization (Commentary) (protein specific)
Crystallization
Organism
micro batch method, cocrystallization of YagE with 2-keto-3-deoxy galactonate and with pyruvate and glyceraldehyde in 100 mM HEPES pH 6.5, 200 mM MgCl2, 15% (w/v) PEG 3350, 50 mM pyruvate, and 50 mM glyceraldehyde
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-dehydro-3-deoxy-D-galactonate
-
716878
Escherichia coli
?
-
-
-
?
pyruvate + glyceraldehyde
-
716878
Escherichia coli
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
x-ray crystallography
Escherichia coli
General Information
General Information
Commentary
Organism
physiological function
overexpression of YagE increases cell viability in the presence of certain bactericidal antibiotics, indicating a putative biological role of YagE as a prophage encoded virulence factor enabling the survival of bacteria in the presence of certain antibiotics
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
physiological function
overexpression of YagE increases cell viability in the presence of certain bactericidal antibiotics, indicating a putative biological role of YagE as a prophage encoded virulence factor enabling the survival of bacteria in the presence of certain antibiotics
Escherichia coli
Other publictions for EC 4.1.2.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
716878
Bhaskar
Identification of biochemical ...
Escherichia coli
Proteins
79
1132-1142
2011
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704334
Reher
The nonphosphorylative Entner- ...
Picrophilus torridus
J. Bacteriol.
192
964-974
2010
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2
691007
Rea
Crystal structure and function ...
Escherichia coli
Biochemistry
47
9955-9965
2008
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1
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7
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2
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6
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7
1
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677338
Li
Purification, crystallization ...
Leptospira interrogans
Acta Crystallogr. Sect. F
62
1269-1270
2006
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1
1
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650974
Izard
Crystal structures of the meta ...
Escherichia coli
EMBO J.
19
3849-3856
2000
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3
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649100
Blackwell
-
Rhombohedral crystals of 2-deh ...
Escherichia coli
Acta Crystallogr. Sect. D
55
1368-1369
1999
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1
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5083
Hubbard
Evolution of enzymatic activit ...
Escherichia coli
Biochemistry
37
14369-14375
1998
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5082
Blumenthal
Specific enzymatic assay for D ...
Escherichia coli
Anal. Biochem.
185
286-293
1990
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5081
Marsh
An enzymatic determination of ...
Escherichia coli, Escherichia coli NCTC 10418
Anal. Biochem.
145
266-272
1985
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5080
Jeffcoat
Bacterial hydrolases and aldol ...
Klebsiella aerogenes
Nature New Biol.
241
186-187
1973
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4971
Wood
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2-Keto-3-deoxy-6-phosphoglucon ...
Escherichia coli, no activity in Bacillus subtilis
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
7
281-302
1972
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5078
Fish
-
2-keto-3-deoxy-D-Glucarate ald ...
Escherichia coli, Escherichia coli CR63MA
Methods Enzymol.
9
529-534
1966
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