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Literature summary for 4.1.2.17 extracted from

  • Choi, I.G.; Cho, C.S.; Cho, Y.; Yu, Y.G.
    Overproduction, purification and characterization of heat stable aldolase from Methanococcus jannaschii, a hyperthermophic archaea (1998), J. Biochem. Mol. Biol., 31, 130-134.
No PubMed abstract available
Search for more literature for 4.1.2.17

Cloned(Commentary)

Cloned (Comment) Organism
-
 Methanocaldococcus jannaschii

General Stability

General Stability Organism
15% isopropyl alcohol, enzyme maintains more than 90% of its activity Methanocaldococcus jannaschii
50% acetonitrile, enzyme maintains more than 90% of its activity Methanocaldococcus jannaschii
6 M, urea, enzyme maintains more than 90% of its activity Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-fuculose 1-phosphate
-
 Methanocaldococcus jannaschii glycerone phosphate + (S)-lactaldehyde
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 80
-
 Methanocaldococcus jannaschii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
 half-life: 24 h in presence of 0.5 M KH2PO4, half-life: 40 min in presence of 10 mM Tris-HCl Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9
-
 Methanocaldococcus jannaschii

pH Range

pH Minimum pH Maximum Comment Organism
6 10 pH 6: about 30% of maximal activity, pH 10: about 35% of maximal activity Methanocaldococcus jannaschii