Literature summary for 4.1.2.14 extracted from

Seo, P.W.; Ryu, H.C.; Gu, D.H.; Park, H.S.; Park, S.Y.; Kim, J.S.
Structures of Zymomonas 2-keto-3-deoxy-6-phosphogluconate aldolase with and without a substrate analog at the phosphate-binding loop (2018), J. Microbiol. Biotechnol., 28, 1339-1345 .

Search for more literature for 4.1.2.14

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 Star (DE3) cells	Zymomonas mobilis subsp. mobilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
apoform and in complex with 3-phosphoglycerate, hanging drop vapor diffusion method, using 25% (w/v) polyethylene glycol 8,000 and 0.1 M Bis-Tris (pH 5.5) for the apoform or 1.6 M ammonium sulfate, 0 .1 M CAPS (pH 10.5), and 0.2 M lithium sulfate for the 3-phosphoglycerate-bound form	Zymomonas mobilis subsp. mobilis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-dehydro-3-deoxy-6-phospho-D-gluconate	Zymomonas mobilis subsp. mobilis	-	pyruvate + D-glyceraldehyde 3-phosphate	-	?
2-dehydro-3-deoxy-6-phospho-D-gluconate	Zymomonas mobilis subsp. mobilis ATCC 31821	-	pyruvate + D-glyceraldehyde 3-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Zymomonas mobilis subsp. mobilis	Q00384	-	-
Zymomonas mobilis subsp. mobilis ATCC 31821	Q00384	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-ion affinity chromatography and ion exchange column chromatography	Zymomonas mobilis subsp. mobilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-dehydro-3-deoxy-6-phospho-D-gluconate	-	Zymomonas mobilis subsp. mobilis	pyruvate + D-glyceraldehyde 3-phosphate	-	?
2-dehydro-3-deoxy-6-phospho-D-gluconate	-	Zymomonas mobilis subsp. mobilis ATCC 31821	pyruvate + D-glyceraldehyde 3-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
2-keto-3-deoxy-6-phosphogluconate aldolase	-	Zymomonas mobilis subsp. mobilis
KDPG aldolase	-	Zymomonas mobilis subsp. mobilis

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Release 2023.1 (January 2023)