Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A149P | tetramers dissociate into subunits with greatly impaired enzymatic activity | Homo sapiens |
A174D | tetramers dissociate into subunits with greatly impaired enzymatic activity, extremely labile mutant, aggregates rapidly | Homo sapiens |
A337V | retained tetrameric structure, altered kinetic properties | Homo sapiens |
L256P | tetramers dissociate into subunits with greatly impaired enzymatic activity | Homo sapiens |
N334K | tetramers dissociate into subunits with greatly impaired enzymatic activity | Homo sapiens |
R303W | retained tetrameric structure, altered kinetic properties | Homo sapiens |
W147R | retained tetrameric structure, altered kinetic properties | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
D-Fructose 1-phosphate | wild-type, 22°C, pH 7.6 | Homo sapiens | |
0.0033 | - |
D-Fructose 1-phosphate | L256P, 22°C, pH 7.6 | Homo sapiens | |
0.004 | - |
D-fructose 1,6-bisphosphate | wild-type, 22°C, pH 7.6 | Homo sapiens | |
0.0059 | - |
D-fructose 1,6-bisphosphate | L256P, 22°C, pH 7.6 | Homo sapiens | |
0.0098 | - |
D-Fructose 1-phosphate | A149P, 22°C, pH 7.6 | Homo sapiens | |
0.022 | - |
D-fructose 1,6-bisphosphate | A337V, 22°C, pH 7.6 | Homo sapiens | |
0.024 | - |
D-Fructose 1-phosphate | A337V, 22°C, pH 7.6 | Homo sapiens | |
0.0266 | - |
D-Fructose 1-phosphate | W147R, 22°C, pH 7.6 | Homo sapiens | |
0.027 | - |
D-fructose 1,6-bisphosphate | A149P, 22°C, pH 7.6 | Homo sapiens | |
0.047 | - |
D-fructose 1,6-bisphosphate | W147R, 22°C, pH 7.6 | Homo sapiens | |
0.33 | - |
D-fructose 1,6-bisphosphate | R303W, 22°C, pH 7.6 | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
L256P mutant exists as tetramer partly dissociated into its subunits, A149P and N334K exist as mixture of protein conformers that encompass all molecular sizes between tetramer and monomer | Homo sapiens |
40000 | 60000 | gel filtration, monomeric form, A149P, N334K, L256P, A337V mutant | Homo sapiens |
158000 | - |
gel filtration, W147R, R303W, A337V mutant | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | Homo sapiens | - |
glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
patients with fructose intolerance | - |
Purification (Comment) | Organism |
---|---|
homogeneity | Homo sapiens |
Storage Stability | Organism |
---|---|
wild-type enzyme: 50% loss of activity after 3 months, mutants: accelerated loss of activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | - |
Homo sapiens | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-Fructose 1-phosphate | - |
Homo sapiens | Glycerone phosphate + D-glyceraldehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | A149P and N334K mutants exist as mixture of protein conformers that encompass all molecular sizes between tetramer and monomer | Homo sapiens |
monomer | A149P and N334K mutants exist as mixture of protein conformers that encompass all molecular sizes between tetramer and monomer | Homo sapiens |
tetramer | L256P mutant exists as tetramer partly dissociated into its subunits, A149P and N334K exist as mixture of protein conformers that encompass all molecular sizes between tetramer and monomer | Homo sapiens |
trimer | A149P and N334K mutants exist as mixture of protein conformers that encompass all molecular sizes between tetramer and monomer | Homo sapiens |