General Stability | Organism |
---|---|
at 1-4 M urea, slight decrease in activity of the immobilized enzyme. In 5 M urea significant decrease in activity after 2 h. In 3 M urea the soluble enzyme unfolded and dissociated totally during 2 h | Sus scrofa |
immobilization by covalent attachment to a polyacrylamide matrix containing carboxylic functional groups increases stability | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
D-fructose 1,6-bisphosphate | immobilized enzyme | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | - |
Sus scrofa | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
soluble enzyme | Sus scrofa |
45 | - |
immobilized enzyme | Sus scrofa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | 25°C: about 90% of maximal activity, 50°C: about 50% of maximal activity, immobilized enzyme | Sus scrofa |
25 | 50 | 25°C: about 95% of maximal activity, 50°C: about 50% of maximal activity, soluble enzyme | Sus scrofa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
about 10% loss of activity of soluble enzyme and immobilized enzyme after 100 min | Sus scrofa |
55 | - |
about 25% loss of activity of the soluble enzyme after 100 min | Sus scrofa |
55 | - |
about 10% loss of activity of the immobilized enzyme after 100 min | Sus scrofa |
60 | - |
about 25% loss of activity of the immobilized enzyme after 100 min | Sus scrofa |
60 | - |
about 50% loss of activity of the soluble enzyme after 100 min | Sus scrofa |
65 | - |
complete inactivation of the soluble enzyme after 40 min, complete inactivation of the immobilized enzyme after 70 min | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
soluble enzyme | Sus scrofa |
7.5 | - |
immobilized enzyme | Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0: about 75% of maximal activity, pH 9.0: about 70% of maximal activity, immobilized enzyme | Sus scrofa |
6 | 9 | pH 6.0: about 85% of maximal activity, pH 9.0: about 60% of maximal activity, soluble enzyme | Sus scrofa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | maximal thermal stability in the pH-range, soluble enzyme | Sus scrofa |
7 | 7.5 | maximal thermal stability in the pH-range, immobilized enzyme | Sus scrofa |