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Literature summary for 4.1.2.10 extracted from
- Purification, immobilization and characterization of (R)-hydroxynitrile lyase from Prunus amygdalus turcomanica seeds and their applicability for synthesis of enantiopure cyanohydrins (2014), J. Mol. Catal. B, 101, 40-46 .
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| the immobilized enzyme preparations (immobilized onto Eupergit CM and Eupergit C 250 L supports) are successfully reused 10times without loss of activity and enantioselectivity | Prunus turcomanica |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
HPLC | Prunus turcomanica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Prunus turcomanica | - |
Lincz. | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Prunus turcomanica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Prunus turcomanica | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-mandelonitrile | - |
Prunus turcomanica | cyanide + benzaldehyde | - |
r |  |
| cyanide + 2-chlorobenzaldehyde | - |
Prunus turcomanica | (R)-2-chloromandelonitrile | - |
r | |
| cyanide + 3,4-dihydroxybenzaldehyde | - |
Prunus turcomanica | (R)-3,4-dihydroxymandelonitrile | - |
r | |
| cyanide + 3-phenylpropionaldehyde | - |
Prunus turcomanica | (R)-2-hydroxy-4-phenyl butyronitrile | - |
r | |
| cyanide + benzaldehyde | - |
Prunus turcomanica | (R)-mandelonitrile | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 25000, SDS-PAGE | Prunus turcomanica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| (R)-hydroxynitrile lyase | - |
Prunus turcomanica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
soluble enzyme and enzyme immobilized onto Eupergit CM and Eupergit C 250 L supports | Prunus turcomanica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
the relative activity of free enzyme decreases rapidly and depletes at the end of 15 days storage time while the immobilized hydroxynitrile lyases are very active at the same conditions with retained initial activities of 67% and 83% for hydroxynitrile lyase immobilized onto Eupergit CM and hydroxynitrile lyase immobilized onto Eupergit C 250 L, respectively. At the end of 30 days storage time, the corresponding retained activities are 48% and 71% for hydroxynitrile lyase immobilized onto Eupergit CM and hydroxynitrile lyase immobilized onto Eupergit C 250 L | Prunus turcomanica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
enzyme immobilized onto Eupergit CM and Eupergit C 250 L supports | Prunus turcomanica |
| 6 | - |
soluble enzyme | Prunus turcomanica |
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