Literature summary for 4.1.2.10 extracted from

Lanfranchi, E.; Grill, B.; Raghoebar, Z.; Van Pelt, S.; Sheldon, R.A.; Steiner, K.; Glieder, A.; Winkler, M.
Production of hydroxynitrile Lyase from Davallia tyermannii (DtHNL) in Komagataella phaffii and its immobilization as a CLEA to generate a robust biocatalyst (2018), ChemBioChem, 19, 312-316 .

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Application

Application	Comment	Organism
synthesis	the enzyme may find application as a biocatalyst for the synthesis of enantiomerically pure cyanohydrins. DtHNL1-CLEA preparation results in a more robust biocatalyst under acidic conditions, which is an interesting feature for the efficient production of enantiomerically pure cyanohydrins	Davallia tyermanii

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli. Recombinant production of DtHNL1 in Komagataella phaffii under control of the strong and tightly regulated AOX1 promoter. Two different shuttle vectors are used, pPpT4_S and pPpB1, to obtain strains with optimal gene copy numbers for efficient heterologous expression	Davallia tyermanii

General Stability

General Stability	Organism
crosslinked enzyme aggregates result in significant enhancement of the biocatalyst stability under acidic conditions (activity is retained after 168 h at pH 2.4)	Davallia tyermanii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
20000	-	SDS-PAGE	Davallia tyermanii

Organism

Organism	UniProt	Comment	Textmining
Davallia tyermanii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Davallia tyermanii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(R)-mandelonitrile	-	Davallia tyermanii	cyanide + benzaldehyde	-	r
cyanide + benzaldehyde	-	Davallia tyermanii	(R)-mandelonitrile	-	r
cyanide + hydroxypivaldehyde	crosslinked enzyme aggregates catalyze synthesis of (R)-hydroxypivaldehyde cyanohydrin under reaction conditions that immediately inactivated non-immobilized enzyme	Davallia tyermanii	(R)-hydroxypivaldehyde cyanohydrin	-	r

Subunits

Subunits	Comment	Organism
?	x * 20000, SDS-PAGE	Davallia tyermanii

Synonyms

Synonyms	Comment	Organism
DtHNL1	-	Davallia tyermanii

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Release 2023.1 (January 2023)