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Literature summary for 4.1.2.10 extracted from

  • Isobe, K.; Kitagawa, A.; Kanamori, K.; Kashiwagi, N.; Matsui, D.; Yamaguchi, T.; Fuhshuku, K.I.; Semba, H.; Asano, Y.
    Characterization of a novel hydroxynitrile lyase from Nandina domestica Thunb (2018), Biosci. Biotechnol. Biochem., 82, 1760-1769 .
    View publication on PubMed
Search for more literature for 4.1.2.10

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Nandina domestica

General Stability

General Stability Organism
the enzyme is stable without the substrate but is remarkably unstable upon incubation with benzaldehyde and KCN Nandina domestica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12.5
-
 benzaldehyde pH 4.0, 25°C, hydroxynitrile lyase L Nandina domestica
19.6
-
 cyanide pH 4.0, 25°C, hydroxynitrile lyase L Nandina domestica

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ strong inhibition, hydroxynitrile lyase L Nandina domestica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24900
-
 SDS-PAGE, hydroxynitrile lyase S Nandina domestica
28000
-
 SDS-PAGE, hydroxynitrile lyase L Nandina domestica
46000 47000 gel filtration, hydroxynitrile lyase S, hydroxynitrile lyase L Nandina domestica

Organism

Organism UniProt Comment Textmining
Nandina domestica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Nandina domestica

Source Tissue

Source Tissue Comment Organism Textmining
leaf the specific activity of young leaves is significantly higher than that of mature leaves Nandina domestica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-mandelonitrile
-
 Nandina domestica cyanide + benzaldehyde
-
 r
cyanide + 2,4-dimethylbenzaldehyde
-
 Nandina domestica (R)-2-hydroxy-2-(2,4-dimethylphenyl)acetonitrile
-
 ?
cyanide + 2-methoxybenzaldehyde
-
 Nandina domestica (R)-2-hydroxy-2-(2-methoxyphenyl)acetonitrile
-
 ?
cyanide + 2-methylbenzaldehyde
-
 Nandina domestica (R)-2-hydroxy-2-(2-methylphenyl)acetonitrile
-
 ?
cyanide + 4-methoxybenzaldehyde
-
 Nandina domestica (2R)-(4-methoxyphenyl)(hydroxy)ethanenitrile
-
 ?
cyanide + 4-methylbenzaldehyde
-
 Nandina domestica (2R)-(4-methylphenyl)(hydroxy)ethanenitrile
-
 ?
cyanide + benzaldehyde
-
 Nandina domestica (R)-mandelonitrile
-
 ?
additional information the enzyme activity toward 2-methylbenzaldehyde, 2,4-dimethylbenzaldehyde and 3-methoxybenzaldehyde is very low Nandina domestica ?
-
 ?

Synonyms

Synonyms Comment Organism
NdHNL
-
 Nandina domestica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
15
-
 synthesis of (R)-mandelonitrile Nandina domestica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 40 5°C: about 80% of maximal activity, 40°C: about 50% of maximal activity, synthesis of (R)-mandelonitrile Nandina domestica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 30 min, pH 6.0, without substrate, more than 80% of the original activity is retained Nandina domestica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 synthesis of (R)-mandelonitrile Nandina domestica
6
-
 degradation of (R)-mandelonitrile Nandina domestica

pH Range

pH Minimum pH Maximum Comment Organism
3.5 4.5 pH 3.5: about 70% of maximal activity, pH 4.5: about 75% of maximal activity, synthesis of (R)-mandelonitrile Nandina domestica
5 6.5 pH 5.0: about 70% of maximal activity, pH 6.5: about 50% of maximal activity, degradation of (R)-mandelonitrile Nandina domestica

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 8 30°C, 30 min, stable Nandina domestica