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Literature summary for 4.1.2.10 extracted from

  • Zheng, Y.; Xu, J.; Wang, H.; Lin, G.; Hong, R.; Yu, H.
    Hydroxynitrile lyase isozymes from Prunus communis identification, characterization and synthetic applications (2017), Adv. Synth. Catal., 359, 1185-1193 .
No PubMed abstract available
Search for more literature for 4.1.2.10

Cloned(Commentary)

Cloned (Comment) Organism
heterologously expression of the His-tagged hydroxynitrile lyase isoenzymes (HNL1, HNL2, HNL4 and HNL5) in Pichia pastoris Prunus dulcis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.09
-
 (R)-4-methylsulfanylbenzaldehyde pH 4.2, 25°C, isoenzyme HNL2 Prunus dulcis
1.16
-
 (R)-mandelonitrile pH 4.2, 25°C, isoenzyme HNL1 Prunus dulcis
1.47
-
 (R)-mandelonitrile pH 4.2, 25°C, isoenzyme HNL2 Prunus dulcis
1.54
-
 (R)-4-methylsulfanylbenzaldehyde pH 4.2, 25°C, isoenzyme HNL4 Prunus dulcis
1.91
-
 (R)-mandelonitrile pH 4.2, 25°C, isoenzyme HNL4 Prunus dulcis
2.29
-
 (R)-4-methylsulfanylbenzaldehyde pH 4.2, 25°C, isoenzyme HNL1 Prunus dulcis
2.97
-
 (R)-4-methylsulfanylbenzaldehyde pH 4.2, 25°C, isoenzyme HNL5 Prunus dulcis
6.36
-
 (R)-mandelonitrile pH 4.2, 25°C, isoenzyme HNL5 Prunus dulcis

Organism

Organism UniProt Comment Textmining
Prunus dulcis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
hydroxynitrile lyase isoenzymes (HNL1, HNL2, HNL4 and HNL5) Prunus dulcis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-mandelonitrile
-
 Prunus dulcis cyanide + benzaldehyde
-
 r
cyanide + (R)-4-methylsulfanylbenzaldehyde
-
 Prunus dulcis (R)-4-methylsulfanyl-mandelonitrile
-
 ?
cyanide + 2,3,4a,8a-tetrahydro-1,4-benzodioxine-6-carbaldehyde isoenzyme HNL5 Prunus dulcis (2R)-hydroxy(2,3,4a,8a-tetrahydro-1,4-benzodioxin-6-yl)acetonitrile
-
 ?
cyanide + 2-chlorobenzaldehyde
-
 Prunus dulcis (R)-2-chloromandelonitrile
-
 r
cyanide + 2-methoxybenzaldehyde
-
 Prunus dulcis (R)-2-methoxymandelonitrile
-
 r
cyanide + 3-chlorobenzaldehyde
-
 Prunus dulcis (R)-3-chloromandelonitrile
-
 r
cyanide + 3-methoxybenzaldehyde
-
 Prunus dulcis (R)-3-methoxymandelonitrile
-
 r
cyanide + 3-phenoxybenzaldehyde
-
 Prunus dulcis (R)-3-phenoxymandelonitrile
-
 r
cyanide + 3-phenylpropanal isoenzyme HNL5 Prunus dulcis (R)-2-hydroxy-4-phenylbutyronitrile
-
 ?
cyanide + 4-fluorobenzaldehyde
-
 Prunus dulcis (R)-4-fluoromandelonitrile
-
 r
cyanide + 4-methoxybenzaldehyde
-
 Prunus dulcis (R)-4-methoxymandelonitrile
-
 r
cyanide + 4-methylbenzaldehyde
-
 Prunus dulcis (R)-4-methylmandelonitrile
-
 r
cyanide + benzaldehyde
-
 Prunus dulcis (R)-mandelonitrile
-
 r
cyanide + furan-2-carbaldehyde isoenzyme HNL5 Prunus dulcis (2R)-(furan-2-yl)(hydroxy)acetonitrile
-
 ?
cyanide + naphthalene-1-carbaldehyde isoenzyme HNL5 Prunus dulcis (2R)-hydroxy(naphthalen-1-yl)acetonitrile
-
 ?
cyanide + naphthalene-2-carbaldehyde isoenzyme HNL5 Prunus dulcis (2R)-hydroxy(naphthalen-2-yl)acetonitrile
-
 ?
cyanide + phenylacetaldehyde isoenzyme HNL5 Prunus dulcis (2R)-2-hydroxy-3-phenylpropanenitrile
-
 ?
cyanide + pyridine-3-carbaldehyde isoenzyme HNL5 Prunus dulcis (2R)-hydroxy(pyridin-3-yl)acetonitrile
-
 ?
cyanide + thiophene-2-carbaldehyde isoenzyme HNL5 Prunus dulcis (2R)-hydroxy(thiophen-2-yl)acetonitrile
-
 ?

Synonyms

Synonyms Comment Organism
HNL1
-
 Prunus dulcis
HNL2
-
 Prunus dulcis
HNL4
-
 Prunus dulcis
HNL5
-
 Prunus dulcis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
26.3
-
 (R)-4-methylsulfanylbenzaldehyde pH 4.2, 25°C, isoenzyme HNL4 Prunus dulcis
40.3
-
 (R)-4-methylsulfanylbenzaldehyde pH 4.2, 25°C, isoenzyme HNL2 Prunus dulcis
139
-
 (R)-4-methylsulfanylbenzaldehyde pH 4.2, 25°C, isoenzyme HNL1 Prunus dulcis
142
-
 (R)-4-methylsulfanylbenzaldehyde pH 4.2, 25°C, isoenzyme HNL5 Prunus dulcis
240
-
 (R)-mandelonitrile pH 4.2, 25°C, isoenzyme HNL1 Prunus dulcis
380
-
 (R)-mandelonitrile pH 4.2, 25°C, isoenzyme HNL2 Prunus dulcis
817
-
 (R)-mandelonitrile pH 4.2, 25°C, isoenzyme HNL5 Prunus dulcis
818
-
 (R)-mandelonitrile pH 4.2, 25°C, isoenzyme HNL4 Prunus dulcis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
17
-
 (R)-4-methylsulfanylbenzaldehyde pH 4.2, 25°C, isoenzyme HNL4 Prunus dulcis
37
-
 (R)-4-methylsulfanylbenzaldehyde pH 4.2, 25°C, isoenzyme HNL2 Prunus dulcis
48
-
 (R)-4-methylsulfanylbenzaldehyde pH 4.2, 25°C, isoenzyme HNL5 Prunus dulcis
61
-
 (R)-4-methylsulfanylbenzaldehyde pH 4.2, 25°C, isoenzyme HNL1 Prunus dulcis
128
-
 (R)-mandelonitrile pH 4.2, 25°C, isoenzyme HNL5 Prunus dulcis
207
-
 (R)-mandelonitrile pH 4.2, 25°C, isoenzyme HNL1 Prunus dulcis
258
-
 (R)-mandelonitrile pH 4.2, 25°C, isoenzyme HNL2 Prunus dulcis
428
-
 (R)-mandelonitrile pH 4.2, 25°C, isoenzyme HNL4 Prunus dulcis