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Literature summary for 4.1.2.10 extracted from

  • Alagöz, D.; Tükel, S.; Yildirim, D.
    Purification, immobilization and characterization of (R)-hydroxynitrile lyase from Prunus amygdalus turcomanica seeds and their applicability for synthesis of enantiopure cyanohydrins (2014), J. Mol. Catal. B, 101, 40-46.
No PubMed abstract available
Search for more literature for 4.1.2.10

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.38
-
 (R)-mandelonitrile native protein, pH 5.5, 25°C Prunus turcomanica
0.95
-
 (R)-mandelonitrile enzyme immobilized onto Eupergit C250 L, pH 5.5, 25°C Prunus turcomanica
1 4 benzaldehyde native protein, pH 5.5, 25°C Prunus turcomanica
1.3
-
 (R)-mandelonitrile enzyme immobilized onto Eupergit CM, pH 5.5, 25°C Prunus turcomanica
38
-
 benzaldehyde enzyme immobilized onto Eupergit C250 L, pH 5.5, 25°C Prunus turcomanica
41
-
 benzaldehyde enzyme immobilized onto Eupergit CM, pH 5.5, 25°C Prunus turcomanica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 4 * 25000, SDS-PAGE Prunus turcomanica
100000
-
 gel filtration Prunus turcomanica

Organism

Organism UniProt Comment Textmining
Prunus turcomanica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Prunus turcomanica

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Prunus turcomanica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-mandelonitrile
-
 Prunus turcomanica cyanide + benzaldehyde
-
 ?
cyanide + 2-chlorobenzaldehyde
-
 Prunus turcomanica (R)-2-chloromandelonitrile
-
 ?
cyanide + 3,4-dihydroxybenzaldehyde
-
 Prunus turcomanica (R)-3,4-dihydroxymandelonitrile
-
 ?
cyanide + 4-phenylbutan-2-one
-
 Prunus turcomanica 2-hydroxy-4-phenylbutyronitrile
-
 ?
cyanide + benzaldehyde
-
 Prunus turcomanica (R)-mandelonitrile
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 25000, SDS-PAGE Prunus turcomanica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5
-
 carboligation reaction Prunus turcomanica
25
-
 mandelonitrile cleavage reaction Prunus turcomanica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 carboligation reaction Prunus turcomanica
5.5
-
 mandelonitrile cleavage reaction, enzyme immobilized onto Eupergit CM and Eupergit C 250 L supports Prunus turcomanica
6
-
 mandelonitrile cleavage reaction, native enzyme Prunus turcomanica