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Literature summary for 4.1.2.10 extracted from

  • Hajnal, I.; Lyskowski, A.; Hanefeld, U.; Gruber, K.; Schwab, H.; Steiner, K.
    Biochemical and structural characterization of a novel bacterial manganese-dependent hydroxynitrile lyase (2013), FEBS J., 280, 5815-5828.
    View publication on PubMed
Search for more literature for 4.1.2.10

Cloned(Commentary)

Cloned (Comment) Organism
-
 Granulicella tundricola

Crystallization (Commentary)

Crystallization (Comment) Organism
to 2.5 A resolution, Structure exhibits a cupin fold, and manganese is bound to three histidine and one glutamine residue Granulicella tundricola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10.3
-
 (R)-mandelonitrile pH 4.5, 25°C Granulicella tundricola

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Granulicella tundricola

Organism

Organism UniProt Comment Textmining
Granulicella tundricola E8WYN5
-
-
Granulicella tundricola MP5ACTX9 E8WYN5
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.74
-
 pH 6.0, 25°C Granulicella tundricola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-mandelonitrile
-
 Granulicella tundricola cyanide + benzaldehyde
-
 r
(R)-mandelonitrile
-
 Granulicella tundricola MP5ACTX9 cyanide + benzaldehyde
-
 r
cyanide + benzaldehyde
-
 Granulicella tundricola (R)-mandelonitrile 90% enantiomeric excess at 80% conversion using 0.5 M benzaldehyde in a biphasic reaction system with methyl tertiary butyl ether r
cyanide + benzaldehyde
-
 Granulicella tundricola MP5ACTX9 (R)-mandelonitrile 90% enantiomeric excess at 80% conversion using 0.5 M benzaldehyde in a biphasic reaction system with methyl tertiary butyl ether r

Synonyms

Synonyms Comment Organism
AciX9_0562 locus name Granulicella tundricola
cupin 2 conserved barrel domain protein
-
 Granulicella tundricola

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 (R)-mandelonitrile pH 4.5, 25°C Granulicella tundricola