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Literature summary for 4.1.2.10 extracted from

  • Fukuta, Y.; Nanda, S.; Kato, Y.; Yurimoto, H.; Sakai, Y.; Komeda, H.; Asano, Y.
    Characterization of a new (R)-hydroxynitrile lyase from the Japanese apricot Prunus mume and cDNA cloning and secretory expression of one of the isozymes in Pichia pastoris (2011), Biosci. Biotechnol. Biochem., 75, 214-220.
    View publication on PubMed
Search for more literature for 4.1.2.10

Cloned(Commentary)

Cloned (Comment) Organism
expresion in Pichia pastoris Prunus mume
expressed in Pichia pastoris strain GS115 Prunus mume

Inhibitors

Inhibitors Comment Organism Structure
2-Iodoacetamide 1 mM, 9% residual activity; 9.0% residual activity at 1 mM Prunus mume
2-mercaptoethanol 87.3% residual activity at 1 mM Prunus mume
Ag+ 1 mM, 8% residual activity; 8.2 residual activity at 1 mM Prunus mume
diethyldicarbonate 35.4% residual activity at 1 mM Prunus mume
Hg2+ 1 mM, 9% residual activity; 9.0% residual activity at 1 mM Prunus mume
iodoacetic acid 1 mM, 8% residual activity; 8.0% residual activity at 1 mM Prunus mume
additional information not inhibited by EDTA, EGTA, sodium fluoride, o-phenanthroline, 2,4-dinitro-1-thiocyanate, NaN3, dithiothreitol, phenylhydrazine, Triton X-100, 2,2'-bipyridyl, N-ethylmaleimide, and D-cycloserine Prunus mume
NH2OH 82.7% residual activity at 1 mM Prunus mume
p-chloromercuribenzoic acid 58.6% residual activity at 10 mM Prunus mume
phenylmethylsulfonyl fluoride 68.5% residual activity at 1 mM Prunus mume

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3
-
 benzaldehyde pH 5.0, 25°C Prunus mume
5.3
-
 benzaldehyde isoform HNL2, at pH 4.5 and 25°C Prunus mume

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 gel filtration Prunus mume
58100
-
 1 * 58100, recombinant (R)-HNL, SDS-PAGE Prunus mume
58100
-
 1 * 58100, SDS-PAGE Prunus mume
62000
-
 1 * 62000, non-glycosylated isoform HNL2, SDS-PAGE Prunus mume
62000
-
 x * 62751, calculated, x * 62000, SDS-PAGE of deglycosylated protein Prunus mume
62751
-
 1 * 62751, isoform HNL2, calculated from amino acid sequence Prunus mume
62751
-
 x * 62751, calculated, x * 62000, SDS-PAGE of deglycosylated protein Prunus mume
97000
-
 1 * 97000, glycosylated isoform HNL2, SDS-PAGE Prunus mume

Organism

Organism UniProt Comment Textmining
Prunus mume
-
-
-
Prunus mume B9X0I1
-
-
Prunus mume B9X0I2 cultivar Nanko
-

Posttranslational Modification

Posttranslational Modification Comment Organism
flavoprotein enzyme shows a typical flavoprotein spectrum with absorption maxima at 389 nm nd 463 nm Prunus mume
glycoprotein molecular mass 97000 Da, mass of deglycosylated protein 62000 Da Prunus mume

Purification (Commentary)

Purification (Comment) Organism
-
 Prunus mume
ammonium sulfate precipitation, DEAE Toyopearl column chromatography, phenyl Toyopearl column chromatography, and Superdex 200 gel filtration Prunus mume

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Prunus mume
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2 20 pH 5.0, 25°C Prunus mume
9
-
 (R)-HNL from crude extract, in 0.3 mM citrate buffer, at pH 5.0 and 25°C Prunus mume
129
-
 isoform HNL2 after purification, in 0.3 mM citrate buffer, at pH 5.0 and 25°C Prunus mume
129
-
 pH 5.0, 25°C Prunus mume
220
-
 (R)-HNL after 24.4fold purification, in 0.3 mM citrate buffer, at pH 5.0 and 25°C Prunus mume

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyanide + benzaldehyde
-
 Prunus mume (R)-mandelonitrile
-
 ?
cyanide + benzaldehyde
-
 Prunus mume (R)-mandelonitrile
-
 r

Subunits

Subunits Comment Organism
? x * 62751, calculated, x * 62000, SDS-PAGE of deglycosylated protein Prunus mume
monomer 1 * 58100, recombinant (R)-HNL, SDS-PAGE Prunus mume
monomer 1 * 62000, non-glycosylated isoform HNL2, SDS-PAGE Prunus mume
monomer 1 * 62751, isoform HNL2, calculated from amino acid sequence Prunus mume
monomer 1 * 97000, glycosylated isoform HNL2, SDS-PAGE Prunus mume
monomer 1 * 58100, SDS-PAGE Prunus mume

Synonyms

Synonyms Comment Organism
(R)-HNL
-
 Prunus mume
(R)-hydroxynitrile lyase
-
 Prunus mume
HNL2
-
 Prunus mume
HNL2 isoform Prunus mume

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 35
-
 Prunus mume

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Prunus mume

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Prunus mume