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Literature summary for 4.1.2.10 extracted from

  • Ueatrongchit, T.; Kayo, A.; Komeda, H.; Asano, Y.; H-Kittikun, A.
    Purification and characterization of a novel (R)-hydroxynitrile lyase from Eriobotrya japonica (Loquat) (2008), Biosci. Biotechnol. Biochem., 72, 1513-1522.
    View publication on PubMed
Search for more literature for 4.1.2.10

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 10 mM, 19% inhibition Rhaphiolepis bibas
AgNO3 1 mM, 100% inhibition Rhaphiolepis bibas
CuSO4 1 mM, 100% inhibition Rhaphiolepis bibas
diethyldicarbonate 1 mM, 36% inhibition Rhaphiolepis bibas
FeCl3 1 mM, 50% inhibition Rhaphiolepis bibas
HgCl2 1 mM, 100% inhibition Rhaphiolepis bibas
iodoacetic acid 1 mM, 53% inhibition Rhaphiolepis bibas
Phenylmethylsulfonylfluoride 1 mM, 59% inhibition Rhaphiolepis bibas

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.161
-
 (R)-mandelonitrile pH 5.5 Rhaphiolepis bibas
1.1
-
 2-naphthaldehyde pH 5.5 Rhaphiolepis bibas
2.6
-
 piperonal pH 5.5 Rhaphiolepis bibas
3.5
-
 p-anisaldehyde pH 5.5 Rhaphiolepis bibas
11.5
-
 benzaldehyde pH 5.5 Rhaphiolepis bibas
39.3
-
 pivaldehyde pH 5.5 Rhaphiolepis bibas
41.8
-
 Isobutyraldehyde pH 5.5 Rhaphiolepis bibas
99.9
-
 propionaldehyde pH 5.5 Rhaphiolepis bibas

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metal ions are not required for its activity Rhaphiolepis bibas

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62300
-
 1 * 62300, SDS-PAGE Rhaphiolepis bibas
72000
-
 gel filtration Rhaphiolepis bibas

Organism

Organism UniProt Comment Textmining
Rhaphiolepis bibas B7VF77
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Rhaphiolepis bibas

Purification (Commentary)

Purification (Comment) Organism
to homogeneity. The final yield, of 36% with 49fold purification, is obtained by 30-80% (NH4)2SO4 fractionation and column chromatography on DEAE-Toyopearl and concanavalin A Sepharose 4B, which suggests the presence of a carbohydrate side chain Rhaphiolepis bibas

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Rhaphiolepis bibas
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
40.9
-
-
 Rhaphiolepis bibas

Storage Stability

Storage Stability Organism
4°C, 20 mM potassium phosphate buffer, pH 6.0, stable for 30 d Rhaphiolepis bibas

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyanide + 2-naphthaldehyde
-
 Rhaphiolepis bibas (R)-2-hydroxy-2-(naphthalen-2-yl)acetonitrile
-
 ?
cyanide + benzaldehyde
-
 Rhaphiolepis bibas (R)-mandelonitrile
-
 r
cyanide + isobutyraldehyde
-
 Rhaphiolepis bibas 2-hydroxy-3-methylbutyronitrile
-
 ?
cyanide + p-anisaldehyde
-
 Rhaphiolepis bibas (R)-2-hydroxy-2-(4-methoxyphenyl)acetonitrile
-
 ?
cyanide + piperonal
-
 Rhaphiolepis bibas (R)-2-hydroxy-2-(3,4-methylenedioxyphenyl)acetonitrile
-
 ?
cyanide + pivaldehyde
-
 Rhaphiolepis bibas pivaloyl cyanide
-
 ?
cyanide + propionaldehyde
-
 Rhaphiolepis bibas 2-hydroxybutyronitrile
-
 ?
additional information the enzyme is active towards aromatic and aliphatic aldehydes and shows a preference for small substrates over bulky one Rhaphiolepis bibas ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 62300, SDS-PAGE Rhaphiolepis bibas

Synonyms

Synonyms Comment Organism
(R)-hydroxynitrile lyase
-
 Rhaphiolepis bibas
EjHNL
-
 Rhaphiolepis bibas
HNL
-
 Rhaphiolepis bibas

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Rhaphiolepis bibas

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 20°C: about 50% of maximal activity, 60°C: about 40% of maximal activity Rhaphiolepis bibas

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 60 min, 68% residual activity Rhaphiolepis bibas
80
-
 60 min, 28% residual activity Rhaphiolepis bibas

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
56
-
 (R)-mandelonitrile pH 5.5 Rhaphiolepis bibas
110
-
 Isobutyraldehyde pH 5.5 Rhaphiolepis bibas
164
-
 pivaldehyde pH 5.5 Rhaphiolepis bibas
223
-
 propionaldehyde pH 5.5 Rhaphiolepis bibas
334
-
 piperonal pH 5.5 Rhaphiolepis bibas
582
-
 p-anisaldehyde pH 5.5 Rhaphiolepis bibas
1160
-
 2-naphthaldehyde pH 5.5 Rhaphiolepis bibas
1700
-
 benzaldehyde pH 5.5 Rhaphiolepis bibas

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Rhaphiolepis bibas

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6 pH 4.5: about 40% of maximal activity, pH 6.0: about 30% of maximal activity Rhaphiolepis bibas

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 60 min, stable Rhaphiolepis bibas

Cofactor

Cofactor Comment Organism Structure
FAD enzyme is a flavoprotein containing FAD as a prosthetic group Rhaphiolepis bibas

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9.6
-
 Isobutyraldehyde pH 5.5 Rhaphiolepis bibas
14.3
-
 pivaldehyde pH 5.5 Rhaphiolepis bibas
19.4
-
 propionaldehyde pH 5.5 Rhaphiolepis bibas
29.2
-
 piperonal pH 5.5 Rhaphiolepis bibas
50.8
-
 p-anisaldehyde pH 5.5 Rhaphiolepis bibas
101
-
 2-naphthaldehyde pH 5.5 Rhaphiolepis bibas
148
-
 benzaldehyde pH 5.5 Rhaphiolepis bibas
348
-
 (R)-mandelonitrile pH 5.5 Rhaphiolepis bibas