Literature summary for 4.1.2.10 extracted from

Wajant, H.; Foerster, S.; Selmar, D.; Effenberger, F.; Pfizenmaier, K.
Purification and characterization of a novel (R)-mandelonitrile lyase from the fern Phlebodium aureum (1995), Plant Physiol., 109, 1231-1238.

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Inhibitors

Inhibitors	Comment	Organism	Structure
AgNO3	0.2 mM, 85% inhibition	Prunus dulcis
diethyl dicarbonate	0.5 mM, 60% inhibition	Phlebodium aureum
diethyl dicarbonate	0.5 mM, 50% inhibition. 2 mM, 79% inhibition	Prunus dulcis
diisopropyl fluorophosphate	2 mM, 80% inhibition	Prunus dulcis
iodoacetamide	2 mM, 70% inhibition	Prunus dulcis
additional information	the enzyme is not inhibited by sulfhydryl- or hydroxyl-modifying reagents	Phlebodium aureum
phenylmethylsulfonyl fluoride	2 mM, 50% inhibition	Prunus dulcis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.83	-	benzaldehyde	pH 5.3	Phlebodium aureum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
20000	-	x * 20000, SDS-PAGE	Phlebodium aureum
168000	-	isoenzyme A and C, gel filtration	Phlebodium aureum

Organism

Organism	UniProt	Comment	Textmining
Phlebodium aureum	-	-	-
Prunus dulcis	O24243	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Phlebodium aureum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cyanide + benzaldehyde	-	Phlebodium aureum	(R)-mandelonitrile	82% yield, 99% enantiomeric excess	?
cyanide + benzaldehyde	-	Prunus dulcis	(R)-mandelonitrile	99% yield, 99% enantiomeric excess	?
cyanide + butanal	-	Prunus dulcis	(2R)-2-hydroxypentanenitrile	100% yield, 90% enantiomeric excess	?
cyanide + butanal	-	Phlebodium aureum	(2R)-2-hydroxypentanenitrile	42% yield, 55% enantiomeric excess	?
cyanide + pentan-2-one	-	Prunus dulcis	(2R)-2-hydroxy-2-methylpentanenitrile	-	?
cyanide + pentan-2-one	-	Phlebodium aureum	(2R)-2-hydroxy-2-methylpentanenitrile	17% yield	?
cyanide + thiophene-2-carbaldehyde	-	Prunus dulcis	(2R)-hydroxy(thiophen-2-yl)ethanenitrile	48% yield, 87% enantiomeric excess	?
cyanide + thiophene-2-carbaldehyde	-	Phlebodium aureum	(2R)-hydroxy(thiophen-2-yl)ethanenitrile	87% yield, 99% enentiomeric excess	?
additional information	aliphatic carbonyls are poorly converted	Phlebodium aureum	?	-	?

Subunits

Subunits	Comment	Organism
multimer	x * 20000, SDS-PAGE	Phlebodium aureum

Synonyms

Synonyms	Comment	Organism
(R)-Mandelonitrile lyase	-	Phlebodium aureum
(R)-Mandelonitrile lyase	-	Prunus dulcis
PaMDL	-	Prunus dulcis
PhaMDL	-	Phlebodium aureum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
35	40	-	Phlebodium aureum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.5	7	because of the base-catalyzed decomposition of mandelonitrile, pH dependence is only determined below pH 7.5. Activity increases from pH 3.5 to 6.2 and levels off between pH 6.2 and 7 for both isoforms	Phlebodium aureum

Cofactor

Cofactor	Comment	Organism	Structure
FAD	a flavoprotein	Prunus dulcis
additional information	not a flavoprotein	Phlebodium aureum

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Release 2023.1 (January 2023)