Literature summary for 4.1.1.B12 extracted from

Xu, B.; Lei, L.; Zhu, X.; Zhou, Y.; Xiao, Y.
Identification and characterization of L-lysine decarboxylase from Huperzia serrata and its role in the metabolic pathway of lycopodium alkaloid (2017), Phytochemistry, 136, 23-30 .

Search for more literature for 4.1.1.B12

Cloned(Commentary)

Cloned (Comment)	Organism
six cDNAs encoding lysine decarboxylases (LDCs) are cloned from Huperzia serrata by degenerate PCR and rapid amplification of cDNA ends (RACE), phylogenetic analysis, one HsLDC isoform is functionally characterized as lysine decarboxylase. Recombinant transient expression of HsLDC in Nicotiana tabacum leaves specifically increase cadaverine content from zero to 0.75 mg per gram of dry mass. Recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)	Huperzia serrata

Cloned (Comment)

Organism

six cDNAs encoding lysine decarboxylases (LDCs) are cloned from Huperzia serrata by degenerate PCR and rapid amplification of cDNA ends (RACE), phylogenetic analysis, one HsLDC isoform is functionally characterized as lysine decarboxylase. Recombinant transient expression of HsLDC in Nicotiana tabacum leaves specifically increase cadaverine content from zero to 0.75 mg per gram of dry mass. Recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)

Huperzia serrata

Protein Variants

Protein Variants	Comment	Organism
K118A	site-directed mutagenesis, the mutant shows about 90% reduced activity compared to the wild-type enzyme	Huperzia serrata

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.194	-	L-methionine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata
0.209	-	L-arginine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata
0.878	-	L-lysine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata
1.07	-	L-norleucine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata
1.32	-	L-cysteine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata
1.43	-	L-ornithine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata
1.64	-	D-Lysine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata
5.77	-	L-valine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata
15.7	-	L-phenylalanine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-lysine	Huperzia serrata	-	cadaverine + CO2	-	?
L-ornithine	Huperzia serrata	-	putrescine + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Huperzia serrata	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
D-lysine	3.0% cataytic efficiency compared to L-lysine	Huperzia serrata	cadaverine + CO2	-	?
L-arginine	19.5% cataytic efficiency compared to L-lysine	Huperzia serrata	agmatine + CO2	-	?
L-cysteine	5.5% cataytic efficiency compared to L-lysine	Huperzia serrata	?	-	?
L-lysine	-	Huperzia serrata	cadaverine + CO2	-	?
L-lysine	best substrate	Huperzia serrata	cadaverine + CO2	-	?
L-methionine	21.0% cataytic efficiency compared to L-lysine	Huperzia serrata	3-(methylsulfanyl)propanamine + CO2	-	?
L-norleucine	4.5% cataytic efficiency compared to L-lysine	Huperzia serrata	?	-	?
L-ornithine	-	Huperzia serrata	putrescine + CO2	-	?
L-ornithine	92% cataytic efficiency compared to L-lysine	Huperzia serrata	putrescine + CO2	-	?
L-phenylalanine	1.2% cataytic efficiency compared to L-lysine	Huperzia serrata	phenylethylamine + CO2	-	?
L-valine	1.8% cataytic efficiency compared to L-lysine	Huperzia serrata	2-methylpropanamine + CO2	-	?

Synonyms

Synonyms	Comment	Organism
HsLDC	-	Huperzia serrata
L-lysine decarboxylase	-	Huperzia serrata
LDC	-	Huperzia serrata

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Huperzia serrata

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.0859	-	L-methionine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata
0.0859	-	L-arginine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata
0.102	-	L-norleucine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata
0.102	-	D-Lysine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata
0.155	-	L-cysteine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata
0.214	-	L-valine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata
0.404	-	L-phenylalanine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata
1.85	-	L-lysine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata
2.78	-	L-ornithine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Huperzia serrata

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Huperzia serrata

General Information

General Information	Comment	Organism
metabolism	the enzyme plays a role in the metabolic pathway of lycopodium alkaloid. Lysine decarboxylation is the first biosynthetic step of Huperzine A (HupA)	Huperzia serrata

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
0.0257	-	L-phenylalanine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata
0.0371	-	L-valine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata
0.0625	-	D-Lysine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata
0.0957	-	L-norleucine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata
0.117	-	L-cysteine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata
0.412	-	L-arginine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata
0.442	-	L-methionine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata
1.94	-	L-ornithine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata
2.11	-	L-lysine	pH 7.5, 37°C, recombinant enzyme	Huperzia serrata