Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.98 extracted from

  • Jacewicz, A.; Izumi, A.; Brunner, K.; Schnell, R.; Schneider, G.
    Structural insights into the UbiD protein family from the crystal structure of PA0254 from Pseudomonas aeruginosa (2013), PLoS ONE, 8, e63161.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.98

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 1 M (NH4)2SO4, 0.1 M Bis-Tris, pH 5.5, 1% (w/v) PEG 3350 or 0.1 M HEPES, pH 7.5, 0.2 M MgCl2, 10% (w/v) PEG 400 Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ contains a Mg2+ binding site Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57614
-
 2 * 57614, SeMet-substituted protein, calculated from amino acid sequence Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose bead chromatography and Sephacryl S200 gel filtration Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-octaprenyl-4-hydroxybenzoate
-
 Pseudomonas aeruginosa 2-octaprenylphenol + CO2
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 57614, SeMet-substituted protein, calculated from amino acid sequence Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
PA0254
-
 Pseudomonas aeruginosa
ubiD
-
 Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
additional information the enzyme does not bind FMN Pseudomonas aeruginosa