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Literature summary for 4.1.1.96 extracted from

  • Nakao, H.; Shinoda, S.; Yamamoto, S.
    Purification and properties of carboxynorspermidine decarboxylase, a novel enzyme involved in norspermidine biosynthesis, from Vibrio alginolyticus (1990), J. Gen. Microbiol., 136, 1699-1704.
No PubMed abstract available
Search for more literature for 4.1.1.96

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol maximum stimulation of activity at more than 5 mM dithiothreitol Vibrio alginolyticus

General Stability

General Stability Organism
freezing and thawing the purified preparation results in almost complete loss of activity. A substantial loss of activity (about 20%) is observed when the enzyme is dialysed for 12 h or stored at 4°C for 2 days without addition of EDTA Vibrio alginolyticus

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ 82% inhibition at 1 mM Vibrio alginolyticus
Fe3+ 88% inhibition at 1 mM Vibrio alginolyticus
Mn2+ 50% inhibition at 1 mM Vibrio alginolyticus
additional information not inhibited by 2,3-diaminopropionic acid, 2,4-diaminobutyric acid, L-ornithine, L-lysine, Mg2+, Ca2+, Cu2+, Cu+, Na+ and K+ Vibrio alginolyticus
Zn2+ 23% inhibition at 1 mM Vibrio alginolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.175
-
 carboxynorspermidine at pH 8.3 and 37°C Vibrio alginolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43500
-
 2 * 43500, SDS-PAGE Vibrio alginolyticus
86000
-
 gel filtration Vibrio alginolyticus

Organism

Organism UniProt Comment Textmining
Vibrio alginolyticus
-
-
-
Vibrio alginolyticus ATCC 17749
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, Sephacryl S-200 gel filtration, and hydroxyapatite column chromatography. The enzyme is greatly stabilized by the addition of both dithiothreitol and EDTA, but not pyridoxal 5'-phosphate, during purification Vibrio alginolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0077
-
 crude extract, at pH 8.3 and 37°C Vibrio alginolyticus
24.1
-
 after 3130fold purification, at pH 8.3 and 37°C Vibrio alginolyticus

Storage Stability

Storage Stability Organism
4°C, at pH 7.5 in the presence of dithiothreitol, EDTA and NaN, at least one month, the enzyme remains stable Vibrio alginolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxynorspermidine
-
 Vibrio alginolyticus bis(3-aminopropyl)amine + CO2
-
 ?
carboxynorspermidine
-
 Vibrio alginolyticus ATCC 17749 bis(3-aminopropyl)amine + CO2
-
 ?
carboxyspermidine alternative substrate, is decarboxylated at almost the same rate as carboxynorspermidine Vibrio alginolyticus spermidine + CO2
-
 ?
carboxyspermidine alternative substrate, is decarboxylated at almost the same rate as carboxynorspermidine Vibrio alginolyticus ATCC 17749 spermidine + CO2
-
 ?
additional information neither 2,3-diaminopropionic acid, 2,4-diaminobutyric acid, L-ornithine nor L-lysine show detectable substrate activity Vibrio alginolyticus ?
-
 ?
additional information neither 2,3-diaminopropionic acid, 2,4-diaminobutyric acid, L-ornithine nor L-lysine show detectable substrate activity Vibrio alginolyticus ATCC 17749 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 43500, SDS-PAGE Vibrio alginolyticus

Synonyms

Synonyms Comment Organism
C-NSPD decarboxylase
-
 Vibrio alginolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Vibrio alginolyticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 55 75% and 51% of the maximum activity remains at 30°C and 45°C, respectively, but the activity decreases rapidly at temperatures above 55°C or below 20°C Vibrio alginolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
-
 Vibrio alginolyticus

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9 57% and 72% of the maximum activity are observed at pH 7.5 and 9.0, respectively Vibrio alginolyticus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate Km value is 0.0048 mM Vibrio alginolyticus

pI Value

Organism Comment pI Value Maximum pI Value
Vibrio alginolyticus isoelectric focusing
-
 4.3

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1
-
 at pH 8.3 and 37°C Vibrio alginolyticus Mn2+