Literature summary for 4.1.1.96 extracted from

Deng, X.; Lee, J.; Michael, A.J.; Tomchick, D.R.; Goldsmith, E.J.; Phillips, M.A.
Evolution of substrate specificity within a diverse family of beta/alpha-barrel-fold basic amino acid decarboxylases: X-ray structure determination of enzymes with specificity for L-arginine and carboxynorspermidine (2010), J. Biol. Chem., 285, 25708-25719.

Search for more literature for 4.1.1.96

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Campylobacter jejuni

Crystallization (Commentary)

Crystallization (Comment)	Organism
co-crystallized with 10 mM norspermidine, hanging drop vapor diffusion method, using 2.75 M ammonium sulfate, 0.1 M Bicine, pH 8.5	Campylobacter jejuni

Protein Variants

Protein Variants	Comment	Organism
K184E	inactive	Campylobacter jejuni

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.1	-	carboxynorspermidine	pH and temperature not specified in the publication	Campylobacter jejuni
4.1	-	carboxyspermidine	pH and temperature not specified in the publication	Campylobacter jejuni

Organism

Organism	UniProt	Comment	Textmining
Campylobacter jejuni	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+ affinity column chromatography and gel filtration	Campylobacter jejuni

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
carboxynorspermidine	-	Campylobacter jejuni	bis(3-aminopropyl)amine + CO2	-	?
carboxyspermidine	-	Campylobacter jejuni	spermidine + CO2	-	?

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Campylobacter jejuni

Synonyms

Synonyms	Comment	Organism
CANSDC	-	Campylobacter jejuni

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.24	-	carboxyspermidine	pH and temperature not specified in the publication	Campylobacter jejuni
0.58	-	carboxynorspermidine	pH and temperature not specified in the publication	Campylobacter jejuni

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Campylobacter jejuni

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Release 2023.1 (January 2023)