Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Campylobacter jejuni |
Crystallization (Comment) | Organism |
---|---|
co-crystallized with 10 mM norspermidine, hanging drop vapor diffusion method, using 2.75 M ammonium sulfate, 0.1 M Bicine, pH 8.5 | Campylobacter jejuni |
Protein Variants | Comment | Organism |
---|---|---|
K184E | inactive | Campylobacter jejuni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
carboxynorspermidine | pH and temperature not specified in the publication | Campylobacter jejuni | |
4.1 | - |
carboxyspermidine | pH and temperature not specified in the publication | Campylobacter jejuni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ affinity column chromatography and gel filtration | Campylobacter jejuni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxynorspermidine | - |
Campylobacter jejuni | bis(3-aminopropyl)amine + CO2 | - |
? | |
carboxyspermidine | - |
Campylobacter jejuni | spermidine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Campylobacter jejuni |
Synonyms | Comment | Organism |
---|---|---|
CANSDC | - |
Campylobacter jejuni |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
carboxyspermidine | pH and temperature not specified in the publication | Campylobacter jejuni | |
0.58 | - |
carboxynorspermidine | pH and temperature not specified in the publication | Campylobacter jejuni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Campylobacter jejuni |