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Literature summary for 4.1.1.92 extracted from
- Novel reversible indole-3-carboxylate decarboxylase catalyzing nonoxidative decarboxylation (2002), Biosci. Biotechnol. Biochem., 66, 2388-2394.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 1 mM, 85% inhibition | Glutamicibacter nicotianae |  |
| 2,2'-bipyridyl | 1 mM, 72% inhibition | Glutamicibacter nicotianae | |
| 8-hydroxyquinoline | 1 mM, 49% inhibition | Glutamicibacter nicotianae | |
| AgNO3 | 1 mM, 50% inhibition | Glutamicibacter nicotianae | |
| CuCl2 | 1 mM, complete inhibition | Glutamicibacter nicotianae | |
| HgCl2 | 1 mM, 69% inhibition | Glutamicibacter nicotianae | |
| hydroxylamine | 1 mM, 35% inhibition | Glutamicibacter nicotianae | |
| N-ethylmaleimide | 1 mM, 97% inhibition | Glutamicibacter nicotianae | |
| NaN3 | 1 mM, 14% inhibition | Glutamicibacter nicotianae | |
| phenylhydrazine | 1 mM, 87% inhibition | Glutamicibacter nicotianae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.631 | - |
indole-3-carboxylate | pH 7.0, 30°C | Glutamicibacter nicotianae | |
| 0.719 | - |
indole | pH 7.0, 30°C | Glutamicibacter nicotianae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 1.3fold activation | Glutamicibacter nicotianae | |
| Mg2+ | 1 mM, 1.9fold activation | Glutamicibacter nicotianae | |
| Mn2+ | 1 mM, 2.1fold activation | Glutamicibacter nicotianae | |
| Zn2+ | 1 mM, 2.1fold activation | Glutamicibacter nicotianae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 258000 | - |
gel filtration | Glutamicibacter nicotianae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| indole-3-carboxylate | Glutamicibacter nicotianae | - |
indole + CO2 | - |
r | |
| indole-3-carboxylate | Glutamicibacter nicotianae FI1612 | - |
indole + CO2 | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glutamicibacter nicotianae | - |
- |
- |
| Glutamicibacter nicotianae FI1612 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Glutamicibacter nicotianae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.224 | - |
pH 7.0, 30°C | Glutamicibacter nicotianae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, cell extract, 80% of the initial activity is lost after 4 days | Glutamicibacter nicotianae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methylindole + CO2 | - |
Glutamicibacter nicotianae | 2-methylindole-3-carboxylate | - |
? | |
| 2-methylindole + CO2 | - |
Glutamicibacter nicotianae FI1612 | 2-methylindole-3-carboxylate | - |
? | |
| indole-3-carboxylate | - |
Glutamicibacter nicotianae | indole + CO2 | - |
r | |
| indole-3-carboxylate | - |
Glutamicibacter nicotianae FI1612 | indole + CO2 | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Glutamicibacter nicotianae |
| 50 | - |
decarboxylation. For the carboxylation reaction, temperatures over 30°C are not appropriate | Glutamicibacter nicotianae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
30 min, stable | Glutamicibacter nicotianae |
| 30 | - |
30 min, 13% loss of initial activity | Glutamicibacter nicotianae |
| 40 | - |
30 min, 35% loss of initial activity | Glutamicibacter nicotianae |
| 50 | - |
30 min, 65% loss of initial activity | Glutamicibacter nicotianae |
| 60 | - |
30 min, complete loss of initial activity | Glutamicibacter nicotianae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
decarboxylation, potassium phosphate buffer | Glutamicibacter nicotianae |
| 8 | - |
carboxylation, Tris-HCl buffer, 100 mM | Glutamicibacter nicotianae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme does not need any cofactor for the decarboxylation and carboxylation reaction | Glutamicibacter nicotianae |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Glutamicibacter nicotianae | induced specifically by indole-3-carboxylate, but not by indole | up |
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Release 2023.1 (January 2023)
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