Protein Variants | Comment | Organism |
---|---|---|
H404A | carboxylases W390A, S398A, and H404A have activities similar to that of wild type | Homo sapiens |
L394R | natural mutant, certain individuals with combined deficiencies of vitamin K-dependent proteins have a mutation, L394R, in their gamma-glutamyl carboxylase causing impaired glutamate binding | Homo sapiens |
additional information | Y395A propeptide affinity is similar to that of wild type, but those of L394R and W399A are 16-22fold less than that of wild type. Results of kinetic studies with a propeptide-containing substrate are consistent with results of propeptide binding and FLEEL kinetics. Although propeptide and vitamin K binding in some mutants are affected, our data provide compelling evidence that glutamate recognition is the primary function of the conserved region around Leu394 | Homo sapiens |
S398A | carboxylases W390A, S398A, and H404A have activities similar to that of wild type | Homo sapiens |
W390A | carboxylases W390A, S398A, and H404A have activities similar to that of wild type | Homo sapiens |
W399A | lower activity than wild type | Homo sapiens |
Y395A | lower activity than wild type | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Boc-(2S,4S)-4-methylglutamic acid-Glu-Val | competitive inhibitor, FLEEL as substrate | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.54 | - |
FLEEL | wild type, pH 7.4, 20°C | Homo sapiens | |
6.49 | - |
FLEEL | L394R, pH 7.4, 20°C | Homo sapiens | |
14.8 | - |
FLEEL | Y395A, pH 7.4, 20°C | Homo sapiens | |
24.3 | - |
FLEEL | W399A, pH 7.4, 20°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | rough | Homo sapiens | 5783 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P38435 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FLEEL + CO2 + O2 + vitamin K hydroquinone | - |
Homo sapiens | ? + vitamin K epoxide + H2O | - |
? | |
L-glutamate + CO2 + O2 + vitamin K hydroquinone | - |
Homo sapiens | gamma-carboxy L-glutamate + vitamin K epoxide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carboxylase | - |
Homo sapiens |
gamma-glutamyl carboxylase | - |
Homo sapiens |
glutamate carboxylase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kcat values relative to wild type are 51% (L394R), 1% (Y395A), and 2% (W399A), pH 7.4, 20°C | Homo sapiens | |
0.0019 | - |
FLEEL | Y395A, pH 7.4, 20°C | Homo sapiens | |
0.0033 | - |
FLEEL | W399A, pH 7.4, 20°C | Homo sapiens | |
0.11 | - |
FLEEL | L394R, pH 7.4, 20°C | Homo sapiens | |
0.21 | - |
FLEEL | wild type, pH 7.4, 20°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
vitamin K | conversion of glutamic acid to gamma-carboxyglutamic acid is coupled with the oxygenation of KH2 to vitamin K 2,3-epoxide and has been referred to as vitamin K epoxidase activity | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Ki value for Boc-(2S,4S)-4-methylglutamic acid-Glu-Val is above 5 mM for W399A, pH 7.4, 20°C | Homo sapiens | |
0.013 | - |
Boc-(2S,4S)-4-methylglutamic acid-Glu-Val | wild type, pH 7.4, 20°C | Homo sapiens | |
1.4 | - |
Boc-(2S,4S)-4-methylglutamic acid-Glu-Val | L394R, pH 7.4, 20°C | Homo sapiens | |
2.1 | - |
Boc-(2S,4S)-4-methylglutamic acid-Glu-Val | Y395A, pH 7.4, 20°C | Homo sapiens |