Cloned (Comment) | Organism |
---|---|
expressed in baculovirus-infected insect cell. Produced His6-tagged carboxylase as a recombinant protein using a baculovirus expression system | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bromoacetyl-FLEEL peptide | the His6-carboxylase is irreversibly inactivated. Up to 85% of the carboxylase activity is lost over a period of 120 min | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
FLEEL | wild-type carboxylase in bovine liver microsomes | Bos taurus | |
1.6 | - |
FLEEL | recombinant His6-carboxylase present in Sf9 cell microsomes | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Bos taurus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
94000 | - |
SDS-PAGE and Western Blot, His6-carboxylase-Ac-FLEEL confirming affinity-purified carboxylase | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + CO2 + O2 + vitamin K hydroquinone | Bos taurus | - |
gamma-carboxy-L-glutamate + vitamin K epoxide + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | Q07175 | - |
- |
Purification (Comment) | Organism |
---|---|
inactivated His6-carboxylase-Ac-FLEEL purified under denaturing conditions by Ni-chelation chromatography followed by preparative polyacrylamide gel electrophoresis | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FLEEL + CO2 + O2 + vitamin KH2 | - |
Bos taurus | ? + vitamin K epoxide + H2O | - |
? | |
L-glutamate + CO2 + O2 + vitamin K hydroquinone | - |
Bos taurus | gamma-carboxy-L-glutamate + vitamin K epoxide + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
gamma-glutamyl carboxylase | - |
Bos taurus |
glutamate carboxylase | - |
Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
FLEEL | wild-type carboxylase in bovine liver microsomes | Bos taurus | |
1.7 | - |
FLEEL | recombinant His6-carboxylase present in Sf9 cell microsomes | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
vitamin K | - |
Bos taurus |