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Literature summary for 4.1.1.90 extracted from

  • Tie, J.; Wu, S.; Jin, D.; Nicchitta, C.V.; Stafford, D.W.
    A topological study of the human gamma-glutamyl carboxylase (2000), Blood, 96, 973-978.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in 293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information 38-BamHI site introduces 2 amino acid residues (glycine and serine) between the hGC fragment and the Lep tag. A 10-amino acid peptide (MDYKDDDDKG), including the FLAG epitope, is introduced to the amino-terminus of the full length of hGC to make FLAG-hGC and a 8-amino acid peptide (DYKDDDDK) is attached to the carboxyl-terminus of the full length of hGC to make hGC-FLAG. The FLAG-tagged hGC cDNA is subcloned into the EcoRI (Escherichia coli RY13) site of the expression vector pCl-neo under control of the cytomegalovirus (CMV) promoter Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm human glutamyl carboxylase spans the membrane at least 5times, with its N-terminus in the cytoplasm and its C-terminus in the lumen of the endoplasmic reticulum Homo sapiens 5737
-
endoplasmic reticulum membrane amino-terminus of the gamma-glutamyl carboxylase is on the cytoplasmic side of the endoplasmic reticulum, while the carboxylterminus is on the lumenal side Homo sapiens 5789
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
proteinase K digestion of the hGC-FLAG reveals a 60 kDa fragment, indicating the lumenal location of the FLAG tag and therefore the carboxyl-terminus of the carboxylase. In contrast, FLAG-hGC does not show a proteinase K–resistant fragment except for the residual undigested full-length carboxylase, which indicates the cytoplasmic location of the FLAG tag and therefore the amino-terminus of the carboxylase Homo sapiens
95000
-
full-length carboxylase Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P38435
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information enzyme catalyzes vitamin K-dependent posttranslational modification of glutamate to gamma-carboxyl glutamate Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + CO2 + O2 + vitamin K hydroquinone
-
Homo sapiens gamma-carboxy L-glutamate + vitamin K epoxide + H2O
-
?

Synonyms

Synonyms Comment Organism
gamma-glutamyl carboxylase polytopic membrane protein Homo sapiens

Cofactor

Cofactor Comment Organism Structure
vitamin K
-
Homo sapiens