Cloned (Comment) | Organism |
---|---|
expressed in 293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | 38-BamHI site introduces 2 amino acid residues (glycine and serine) between the hGC fragment and the Lep tag. A 10-amino acid peptide (MDYKDDDDKG), including the FLAG epitope, is introduced to the amino-terminus of the full length of hGC to make FLAG-hGC and a 8-amino acid peptide (DYKDDDDK) is attached to the carboxyl-terminus of the full length of hGC to make hGC-FLAG. The FLAG-tagged hGC cDNA is subcloned into the EcoRI (Escherichia coli RY13) site of the expression vector pCl-neo under control of the cytomegalovirus (CMV) promoter | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | human glutamyl carboxylase spans the membrane at least 5times, with its N-terminus in the cytoplasm and its C-terminus in the lumen of the endoplasmic reticulum | Homo sapiens | 5737 | - |
endoplasmic reticulum membrane | amino-terminus of the gamma-glutamyl carboxylase is on the cytoplasmic side of the endoplasmic reticulum, while the carboxylterminus is on the lumenal side | Homo sapiens | 5789 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
proteinase K digestion of the hGC-FLAG reveals a 60 kDa fragment, indicating the lumenal location of the FLAG tag and therefore the carboxyl-terminus of the carboxylase. In contrast, FLAG-hGC does not show a proteinase Kresistant fragment except for the residual undigested full-length carboxylase, which indicates the cytoplasmic location of the FLAG tag and therefore the amino-terminus of the carboxylase | Homo sapiens |
95000 | - |
full-length carboxylase | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P38435 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | enzyme catalyzes vitamin K-dependent posttranslational modification of glutamate to gamma-carboxyl glutamate | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + CO2 + O2 + vitamin K hydroquinone | - |
Homo sapiens | gamma-carboxy L-glutamate + vitamin K epoxide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gamma-glutamyl carboxylase | polytopic membrane protein | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
vitamin K | - |
Homo sapiens |