Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.86 extracted from

  • Yamamoto, S.; Suemoto, Y.; Seito, Y.; Nakao, H.; Shinoda, S.
    The presence of L-2,4-diaminobutyric acid decarboxylase activity in Vibrio species: a new biosynthetic pathway for 1,3-diaminopropane (1986), FEMS Microbiol. Lett., 35, 289-293.
No PubMed abstract available
Search for more literature for 4.1.1.86

General Stability

General Stability Organism
bovine serum albumin stabilizes at below 0.1 mg/ml Vibrio alginolyticus

Inhibitors

Inhibitors Comment Organism Structure
Co2+ highly inhibitory at 20 mM Vibrio alginolyticus
Cu2+ highly inhibitory at 20 mM Vibrio alginolyticus
Fe2+ highly inhibitory at 20 mM Vibrio alginolyticus
Mn2+ highly inhibitory at 20 mM Vibrio alginolyticus
additional information no inhibition by N-ethylmaleimide at up to 20 mM Vibrio alginolyticus
Pb2+ highly inhibitory at 20 mM Vibrio alginolyticus
Sn2+ highly inhibitory at 20 mM Vibrio alginolyticus
Zn2+ highly inhibitory at 20 mM Vibrio alginolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 L-2,4-diaminobutanoate pH 8.3, 37°C Vibrio alginolyticus
0.3
-
 DL-2,4-diaminobutanoate pH 8.3, 37°C Vibrio alginolyticus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Salinivibrio costicola
Mg2+
-
 Vibrio proteolyticus
Mg2+
-
 Vibrio cholerae serotype O1
Mg2+
-
 Vibrio anguillarum
Mg2+ 30% activation at 20 mM Vibrio alginolyticus
additional information enzyme is not affected by Ca2+ Vibrio alginolyticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-2,4-diaminobutanoate Salinivibrio costicola
-
 propane-1,3-diamine + CO2
-
 ?
L-2,4-diaminobutanoate Vibrio proteolyticus
-
 propane-1,3-diamine + CO2
-
 ?
L-2,4-diaminobutanoate Vibrio cholerae serotype O1
-
 propane-1,3-diamine + CO2
-
 ?
L-2,4-diaminobutanoate Vibrio alginolyticus
-
 propane-1,3-diamine + CO2
-
 ?
L-2,4-diaminobutanoate Vibrio anguillarum
-
 propane-1,3-diamine + CO2
-
 ?
L-2,4-diaminobutanoate Vibrio alginolyticus ATCC 17749
-
 propane-1,3-diamine + CO2
-
 ?
L-2,4-diaminobutanoate Vibrio anguillarum NCMB6
-
 propane-1,3-diamine + CO2
-
 ?
L-2,4-diaminobutanoate Vibrio proteolyticus ATCC 15338
-
 propane-1,3-diamine + CO2
-
 ?
L-2,4-diaminobutanoate Vibrio cholerae serotype O1 NCTC4716
-
 propane-1,3-diamine + CO2
-
 ?
L-2,4-diaminobutanoate Salinivibrio costicola NCMB701
-
 propane-1,3-diamine + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Salinivibrio costicola
-
-
-
Salinivibrio costicola NCMB701
-
-
-
Vibrio alginolyticus
-
 ATCC17749
-
Vibrio alginolyticus ATCC 17749
-
 ATCC17749
-
Vibrio anguillarum
-
-
-
Vibrio anguillarum NCMB6
-
-
-
Vibrio cholerae serotype O1
-
 Non-O1
-
Vibrio cholerae serotype O1 NCTC4716
-
 Non-O1
-
Vibrio proteolyticus
-
 strain ATCC15338
-
Vibrio proteolyticus ATCC 15338
-
 strain ATCC15338
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 22.7fold by ammonium sulfate fractionation and anion exchange chromatography Vibrio alginolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00036
-
 cell extract Vibrio anguillarum
0.00071
-
 cell extract Vibrio cholerae serotype O1
0.0011
-
 cell extract Vibrio proteolyticus
0.0014
-
 cell extract Salinivibrio costicola
29.5
-
 partially purified enzyme Vibrio alginolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-2,4-diaminobutanoate
-
 Vibrio alginolyticus propane-1,3-diamine + CO2
-
 ?
DL-2,4-diaminobutanoate
-
 Vibrio alginolyticus ATCC 17749 propane-1,3-diamine + CO2
-
 ?
L-2,4-diaminobutanoate
-
 Salinivibrio costicola propane-1,3-diamine + CO2
-
 ?
L-2,4-diaminobutanoate
-
 Vibrio proteolyticus propane-1,3-diamine + CO2
-
 ?
L-2,4-diaminobutanoate
-
 Vibrio cholerae serotype O1 propane-1,3-diamine + CO2
-
 ?
L-2,4-diaminobutanoate
-
 Vibrio alginolyticus propane-1,3-diamine + CO2
-
 ?
L-2,4-diaminobutanoate
-
 Vibrio anguillarum propane-1,3-diamine + CO2
-
 ?
L-2,4-diaminobutanoate stereospecific reaction, no activity with the D-isomer Vibrio alginolyticus propane-1,3-diamine + CO2 product identification ?
L-2,4-diaminobutanoate
-
 Vibrio alginolyticus ATCC 17749 propane-1,3-diamine + CO2
-
 ?
L-2,4-diaminobutanoate stereospecific reaction, no activity with the D-isomer Vibrio alginolyticus ATCC 17749 propane-1,3-diamine + CO2 product identification ?
L-2,4-diaminobutanoate
-
 Vibrio anguillarum NCMB6 propane-1,3-diamine + CO2
-
 ?
L-2,4-diaminobutanoate
-
 Vibrio proteolyticus ATCC 15338 propane-1,3-diamine + CO2
-
 ?
L-2,4-diaminobutanoate
-
 Vibrio cholerae serotype O1 NCTC4716 propane-1,3-diamine + CO2
-
 ?
L-2,4-diaminobutanoate
-
 Salinivibrio costicola NCMB701 propane-1,3-diamine + CO2
-
 ?

Synonyms

Synonyms Comment Organism
DABA decarboxylase
-
 Salinivibrio costicola
DABA decarboxylase
-
 Vibrio proteolyticus
DABA decarboxylase
-
 Vibrio cholerae serotype O1
DABA decarboxylase
-
 Vibrio alginolyticus
DABA decarboxylase
-
 Vibrio anguillarum
L-2,4-diaminobutyric acid decarboxylase
-
 Salinivibrio costicola
L-2,4-diaminobutyric acid decarboxylase
-
 Vibrio proteolyticus
L-2,4-diaminobutyric acid decarboxylase
-
 Vibrio cholerae serotype O1
L-2,4-diaminobutyric acid decarboxylase
-
 Vibrio alginolyticus
L-2,4-diaminobutyric acid decarboxylase
-
 Vibrio anguillarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Salinivibrio costicola
37
-
 assay at Vibrio proteolyticus
37
-
 assay at Vibrio cholerae serotype O1
37
-
 assay at Vibrio anguillarum
45
-
-
 Vibrio alginolyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 completely stable for up to 90 min Vibrio alginolyticus
45
-
 loss of 18% and 55% after 30 min and 2 h, respectively Vibrio alginolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Vibrio alginolyticus
8.3
-
 assay at Salinivibrio costicola
8.3
-
 assay at Vibrio proteolyticus
8.3
-
 assay at Vibrio cholerae serotype O1
8.3
-
 assay at Vibrio anguillarum

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Salinivibrio costicola
pyridoxal 5'-phosphate
-
 Vibrio proteolyticus
pyridoxal 5'-phosphate
-
 Vibrio cholerae serotype O1
pyridoxal 5'-phosphate
-
 Vibrio alginolyticus
pyridoxal 5'-phosphate
-
 Vibrio anguillarum