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Literature summary for 4.1.1.86 extracted from

  • Yamamoto, S.; Mutoh, N.; Tsuzuki, D.; Ikai, H.; Nakao, H.; Shinoda, S.; Narimatsu, S.; Miyoshi, S.I.
    Cloning and characterization of the ddc homolog encoding L-2,4-diaminobutyrate decarboxylase in Enterobacter aerogenes (2000), Biol. Pharm. Bull., 23, 649-653.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
gene ddc, DNA and amino acid sequence determination and analysis, restriction mapping, expression in Escherichia coli strain HB101 Klebsiella aerogenes

Protein Variants

Protein Variants Comment Organism
K198R site-directed mutagenesis, mutant shows 96% of the wild-type activity Klebsiella aerogenes
K307R site-directed mutagenesis, mutant is catalytically inactive and shows slightly reduced molecular weight compared to the wild-type enzyme due to lacking pyridoxal 5'-phosphate Klebsiella aerogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53659
-
 x * 53659, DNA sequence calculation Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-2,4-diaminobutanoate Klebsiella aerogenes
-
 propane-1,3-diamine + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes Q9S0P8 gene ddc
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2,4-diaminobutanoate
-
 Klebsiella aerogenes propane-1,3-diamine + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 53659, DNA sequence calculation Klebsiella aerogenes

Synonyms

Synonyms Comment Organism
DABA DC
-
 Klebsiella aerogenes
L-2,4-diaminobutyrate decarboxylase
-
 Klebsiella aerogenes

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate binds to Lys307 Klebsiella aerogenes