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Literature summary for 4.1.1.86 extracted from
- Occurrence of a novel L-2,4-diaminobutyrate decarboxylase activity in some species of Enterobacteriaceae, and purification and characterization of the enzymes of Enterobacter aerogenes and Serratia marcescens (1996), Biol. Pharm. Bull., 19, 1298-1303.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activation by ATP or GTP | Klebsiella aerogenes | |
| additional information | no activation by ATP or GTP | Serratia marcescens |
General Stability
| General Stability | Organism |
|---|---|
| freezing and thawing of purified enzyme causes severe loss of activity | Klebsiella aerogenes |
| freezing and thawing of purified enzyme causes severe loss of activity | Serratia marcescens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Klebsiella aerogenes |  |
| Co2+ | - |
Serratia marcescens | |
| Mn2+ | - |
Klebsiella aerogenes | |
| Mn2+ | - |
Serratia marcescens | |
| Ni2+ | - |
Klebsiella aerogenes | |
| Ni2+ | - |
Serratia marcescens | |
| Zn2+ | - |
Klebsiella aerogenes | |
| Zn2+ | - |
Serratia marcescens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
Mg2+ | pH 8.0, 37°C | Klebsiella aerogenes | |
| 0.32 | - |
L-2,4-diaminobutanoate | pH 8.0, 37°C | Serratia marcescens | |
| 1.07 | - |
L-2,4-diaminobutanoate | pH 8.0, 37°C | Klebsiella aerogenes | |
| 1.2 | - |
Mg2+ | pH 8.0, 37°C | Serratia marcescens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | about 65% of the activity with Mg2+ | Klebsiella aerogenes | |
| Ca2+ | about 65% of the activity with Mg2+ | Serratia marcescens | |
| Mg2+ | required, activates 17fold at 10 mM | Serratia marcescens | |
| Mg2+ | required, activates 7fold at 10 mM | Klebsiella aerogenes | |
| Sr2+ | about 65% of the activity with Mg2+ | Klebsiella aerogenes | |
| Sr2+ | about 65% of the activity with Mg2+ | Serratia marcescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
2 * 51000, SDS-PAGE | Klebsiella aerogenes |
| 51000 | - |
2 * 51000, SDS-PAGE | Serratia marcescens |
| 100000 | - |
gel filtration | Klebsiella aerogenes |
| 100000 | - |
gel filtration | Serratia marcescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-2,4-diaminobutanoate | Enterobacter cloacae | - |
propane-1,3-diamine + CO2 | - |
? | |
| L-2,4-diaminobutanoate | Klebsiella aerogenes | - |
propane-1,3-diamine + CO2 | - |
? | |
| L-2,4-diaminobutanoate | Serratia marcescens | - |
propane-1,3-diamine + CO2 | - |
? | |
| L-2,4-diaminobutanoate | Klebsiella pneumoniae | - |
propane-1,3-diamine + CO2 | - |
? | |
| L-2,4-diaminobutanoate | Pantoea agglomerans | - |
propane-1,3-diamine + CO2 | - |
? | |
| L-2,4-diaminobutanoate | Citrobacter freundii | - |
propane-1,3-diamine + CO2 | - |
? | |
| L-2,4-diaminobutanoate | Klebsiella oxytoca | - |
propane-1,3-diamine + CO2 | - |
? | |
| L-2,4-diaminobutanoate | Serratia liquefaciens | - |
propane-1,3-diamine + CO2 | - |
? | |
| L-2,4-diaminobutanoate | Serratia liquefaciens RM 2-Ia | - |
propane-1,3-diamine + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrobacter freundii | - |
strain ATCC 8090 | - |
| Enterobacter cloacae | - |
strain ATCC 13047 | - |
| Klebsiella aerogenes | - |
strain ATCC 13048 | - |
| Klebsiella oxytoca | - |
strain ATCC 13182 | - |
| Klebsiella pneumoniae | - |
strain ATCC 13882 | - |
| Pantoea agglomerans | - |
strain ATCC 27155 | - |
| Serratia liquefaciens | - |
- |
- |
| Serratia liquefaciens RM 2-Ia | - |
- |
- |
| Serratia marcescens | - |
strain ATCC 13880 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 1750fold to homogeneity by two steps of ion exchange chromatography, gel filtration, hydroxyapatite chromatography, and another two steps of ion exchange chromatography | Klebsiella aerogenes |
| native enzyme 886fold to homogeneity by two steps of ion exchange chromatography, gel filtration, hydroxyapatite chromatography, and another two steps of ion exchange chromatography | Serratia marcescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0022 | - |
enzyme in cell extract | Citrobacter freundii |
| 0.0039 | - |
enzyme in cell extract | Pantoea agglomerans |
| 0.00392 | - |
enzyme in cell extract | Serratia liquefaciens |
| 0.0042 | - |
enzyme in cell extract | Klebsiella oxytoca |
| 0.005 | - |
enzyme in cell extract | Klebsiella aerogenes |
| 0.0051 | - |
enzyme in cell extract | Serratia marcescens |
| 0.0052 | - |
enzyme in cell extract | Enterobacter cloacae |
| 0.0071 | - |
enzyme in cell extract | Klebsiella pneumoniae |
| 3.9 | - |
purified enzyme | Serratia marcescens |
| 11.8 | - |
purified enzyme | Klebsiella aerogenes |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 1°C, purified enzyme, 20 mM potassium phosphate, pH 7.5, 0.04 mM pyridoxal 5'-phosphate, and 0.02% NaN3, 2 weeks without loss of activity | Klebsiella aerogenes |
| 1°C, purified enzyme, 20 mM potassium phosphate, pH 7.5, 0.04 mM pyridoxal 5'-phosphate, and 0.02% NaN3, loss of 65% activity within 1 week | Serratia marcescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-2,4-diaminobutanoate | - |
Enterobacter cloacae | propane-1,3-diamine + CO2 | - |
? | |
| L-2,4-diaminobutanoate | - |
Klebsiella aerogenes | propane-1,3-diamine + CO2 | - |
? | |
| L-2,4-diaminobutanoate | - |
Serratia marcescens | propane-1,3-diamine + CO2 | - |
? | |
| L-2,4-diaminobutanoate | - |
Klebsiella pneumoniae | propane-1,3-diamine + CO2 | - |
? | |
| L-2,4-diaminobutanoate | - |
Pantoea agglomerans | propane-1,3-diamine + CO2 | - |
? | |
| L-2,4-diaminobutanoate | - |
Citrobacter freundii | propane-1,3-diamine + CO2 | - |
? | |
| L-2,4-diaminobutanoate | - |
Klebsiella oxytoca | propane-1,3-diamine + CO2 | - |
? | |
| L-2,4-diaminobutanoate | - |
Serratia liquefaciens | propane-1,3-diamine + CO2 | - |
? | |
| L-2,4-diaminobutanoate | - |
Serratia liquefaciens RM 2-Ia | propane-1,3-diamine + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 51000, SDS-PAGE | Klebsiella aerogenes |
| dimer | 2 * 51000, SDS-PAGE | Serratia marcescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DABA DC | - |
Enterobacter cloacae |
| DABA DC | - |
Klebsiella aerogenes |
| DABA DC | - |
Serratia marcescens |
| DABA DC | - |
Klebsiella pneumoniae |
| DABA DC | - |
Pantoea agglomerans |
| DABA DC | - |
Citrobacter freundii |
| DABA DC | - |
Klebsiella oxytoca |
| DABA DC | - |
Serratia liquefaciens |
| L-2,4-diaminobutyrate decarboxylase | - |
Enterobacter cloacae |
| L-2,4-diaminobutyrate decarboxylase | - |
Klebsiella aerogenes |
| L-2,4-diaminobutyrate decarboxylase | - |
Serratia marcescens |
| L-2,4-diaminobutyrate decarboxylase | - |
Klebsiella pneumoniae |
| L-2,4-diaminobutyrate decarboxylase | - |
Pantoea agglomerans |
| L-2,4-diaminobutyrate decarboxylase | - |
Citrobacter freundii |
| L-2,4-diaminobutyrate decarboxylase | - |
Klebsiella oxytoca |
| L-2,4-diaminobutyrate decarboxylase | - |
Serratia liquefaciens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 40 | - |
Klebsiella aerogenes |
| 37 | 40 | - |
Serratia marcescens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable up to, purified enzyme | Klebsiella aerogenes |
| 40 | - |
stable up to, purified enzyme | Serratia marcescens |
| 50 | - |
above, rapid inactivation | Klebsiella aerogenes |
| 50 | - |
above, rapid inactivation | Serratia marcescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.2 | - |
Klebsiella aerogenes |
| 8 | 8.2 | - |
Serratia marcescens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 8.2 | narrow pH range, the enzyme is almost inactive at pH 7.0 | Klebsiella aerogenes |
| 7.2 | 8.2 | narrow pH range, the enzyme is almost inactive at pH 7.0 | Serratia marcescens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | stable for 12 h at 4°C in 20 mM potassium phosphate | Klebsiella aerogenes |
| 6.5 | 7.5 | stable for 12 h at 4°C in 20 mM potassium phosphate | Serratia marcescens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Enterobacter cloacae | |
| pyridoxal 5'-phosphate | - |
Klebsiella aerogenes | |
| pyridoxal 5'-phosphate | - |
Serratia marcescens | |
| pyridoxal 5'-phosphate | - |
Klebsiella pneumoniae | |
| pyridoxal 5'-phosphate | - |
Pantoea agglomerans | |
| pyridoxal 5'-phosphate | - |
Citrobacter freundii | |
| pyridoxal 5'-phosphate | - |
Klebsiella oxytoca | |
| pyridoxal 5'-phosphate | - |
Serratia liquefaciens | |
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