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Literature summary for 4.1.1.84 extracted from

  • Odh, G.; Hindemith, A.; Rosengren, A-M.; Rosengren, E.; Rorsman, H.
    Isolation of a new tautomerase monitored by the conversion of D-dopachrome to 5,6-dihydroxyindole (1993), Biochem. Biophys. Res. Commun., 197, 619-624.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
 D-dopachrome pH 6.0, 25°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12000
-
 3 * 12000, SDS-PAGE Rattus norvegicus
37000
-
 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P80254
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.5
-
 pH 6.0, 25°C Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, pH 5.5-9.0, several weeks, stable Rattus norvegicus
25°C, overnight, stable Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-dopachrome
-
 Rattus norvegicus 5,6-dihydroxyindole + CO2
-
 ?
additional information no substrates are: L-dopachrome, dopaminochrome, L-alpha-methyldopachrome, D-alpha-methyldopachrome Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 12000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 5 min, loss of activity Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 9 broad Rattus norvegicus