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Literature summary for 4.1.1.83 extracted from

  • Selmer, T.; Andrei, P.I.
    p-Hydroxyphenylacetate decarboxylase from Clostridium difficile. A novel glycyl radical enzyme catalysing the formation of p-cresol (2001), Eur. J. Biochem., 268, 1363-1372.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
(3,4-dihydroxyphenyl)acetate
-
Clostridioides difficile
4-hydroxymandelate
-
Clostridioides difficile
4-Hydroxyphenylacetamide competitive Clostridioides difficile
NaCl 800 mM, 50% inactivation Clostridioides difficile
O2 readily and irreversibly inhibited Clostridioides difficile

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
(3,4-dihydroxyphenyl)acetate
-
Clostridioides difficile
2.8
-
(4-hydroxyphenyl)acetate
-
Clostridioides difficile

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
105000
-
alpha,alpha‘, the smaller unit is a C-terminally truncated form, 1 * 110000 + 1 * 105000, SDS-PAGE Clostridioides difficile
110000
-
alpha,alpha‘, the smaller unit is a C-terminally truncated form, 1 * 110000 + 1 * 105000, SDS-PAGE Clostridioides difficile
200000
-
gel filtration Clostridioides difficile

Organism

Organism UniProt Comment Textmining
Clostridioides difficile
-
DSMZ 1296T
-

Oxidation Stability

Oxidation Stability Organism
readily and irreversibly inhibited by O2 Clostridioides difficile

Purification (Commentary)

Purification (Comment) Organism
-
Clostridioides difficile

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.315
-
-
Clostridioides difficile

Storage Stability

Storage Stability Organism
0°C, 100 mM Tris/HCl, pH 7.5, 5 mM ammonium sulfate, 1 mM magnesium chloride, more than 90% of the activity is recovered after 5 days Clostridioides difficile

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3,4-dihydroxyphenyl)acetate + H+
-
Clostridioides difficile 4-methylcatechol + CO2
-
?
(4-hydroxyphenyl)acetate + H+
-
Clostridioides difficile 4-methylphenol + CO2
-
?

Subunits

Subunits Comment Organism
dimer alpha,alpha‘, the smaller unit is a C-terminally truncated form, 1 * 110000 + 1 * 105000, SDS-PAGE Clostridioides difficile

Synonyms

Synonyms Comment Organism
4-Hpd
-
Clostridioides difficile
p-Hpd
-
Clostridioides difficile
p-hydroxyphenylacetate decarboxylase
-
Clostridioides difficile
pHPA decarboxylase
-
Clostridioides difficile

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
half-life: 15 min, in presence of 1 mM sodium sulfide and 5 mM ammonium sulfate Clostridioides difficile

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 0°C in presence of 1 mM sodium sulfide and 5 mM ammonium sulfate Clostridioides difficile

Cofactor

Cofactor Comment Organism Structure
additional information indications for an as yet unidentified low molecular weight cofactor that is required for catalytic activity Clostridioides difficile

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.4
-
(3,4-dihydroxyphenyl)acetate
-
Clostridioides difficile
0.48
-
4-hydroxymandelate
-
Clostridioides difficile
0.7
-
4-Hydroxyphenylacetamide
-
Clostridioides difficile