Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(3,4-dihydroxyphenyl)acetate | - |
Clostridioides difficile | |
4-hydroxymandelate | - |
Clostridioides difficile | |
4-Hydroxyphenylacetamide | competitive | Clostridioides difficile | |
NaCl | 800 mM, 50% inactivation | Clostridioides difficile | |
O2 | readily and irreversibly inhibited | Clostridioides difficile |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
(3,4-dihydroxyphenyl)acetate | - |
Clostridioides difficile | |
2.8 | - |
(4-hydroxyphenyl)acetate | - |
Clostridioides difficile |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
105000 | - |
alpha,alpha, the smaller unit is a C-terminally truncated form, 1 * 110000 + 1 * 105000, SDS-PAGE | Clostridioides difficile |
110000 | - |
alpha,alpha, the smaller unit is a C-terminally truncated form, 1 * 110000 + 1 * 105000, SDS-PAGE | Clostridioides difficile |
200000 | - |
gel filtration | Clostridioides difficile |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridioides difficile | - |
DSMZ 1296T | - |
Oxidation Stability | Organism |
---|---|
readily and irreversibly inhibited by O2 | Clostridioides difficile |
Purification (Comment) | Organism |
---|---|
- |
Clostridioides difficile |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.315 | - |
- |
Clostridioides difficile |
Storage Stability | Organism |
---|---|
0°C, 100 mM Tris/HCl, pH 7.5, 5 mM ammonium sulfate, 1 mM magnesium chloride, more than 90% of the activity is recovered after 5 days | Clostridioides difficile |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3,4-dihydroxyphenyl)acetate + H+ | - |
Clostridioides difficile | 4-methylcatechol + CO2 | - |
? | |
(4-hydroxyphenyl)acetate + H+ | - |
Clostridioides difficile | 4-methylphenol + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha,alpha, the smaller unit is a C-terminally truncated form, 1 * 110000 + 1 * 105000, SDS-PAGE | Clostridioides difficile |
Synonyms | Comment | Organism |
---|---|---|
4-Hpd | - |
Clostridioides difficile |
p-Hpd | - |
Clostridioides difficile |
p-hydroxyphenylacetate decarboxylase | - |
Clostridioides difficile |
pHPA decarboxylase | - |
Clostridioides difficile |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
half-life: 15 min, in presence of 1 mM sodium sulfide and 5 mM ammonium sulfate | Clostridioides difficile |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | 0°C in presence of 1 mM sodium sulfide and 5 mM ammonium sulfate | Clostridioides difficile |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | indications for an as yet unidentified low molecular weight cofactor that is required for catalytic activity | Clostridioides difficile |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
(3,4-dihydroxyphenyl)acetate | - |
Clostridioides difficile | |
0.48 | - |
4-hydroxymandelate | - |
Clostridioides difficile | |
0.7 | - |
4-Hydroxyphenylacetamide | - |
Clostridioides difficile |