Any feedback?
Literature summary for 4.1.1.82 extracted from
- Characterization of recombinant thiamine diphosphate-dependent phosphonopyruvate decarboxylase from Streptomyces viridochromogenes Tü494 (2009), J. Mol. Catal. B, 61, 39-46.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as a recombinant fusion protein with an N-terminal 10x histidine-tag in Escherichia coli BL21(DE3) cells | Streptomyces viridochromogenes |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D297E | catalytic turnover rate is 1.5fold less and Km is increased about 13fold compared to the wild type enzyme | Streptomyces viridochromogenes |
| D297N | catalytic turnover rate of D297N is 2fold lower and Km is increased 940fold compared to the wild type enzyme | Streptomyces viridochromogenes |
| G94A | compared to wild type PPDC, Km is increased only 2fold, while kcat is 4fold lower | Streptomyces viridochromogenes |
| H110A | no detectable activity | Streptomyces viridochromogenes |
| H111A | the mutation has no significant influence on the steady state kinetic constants compared to the wild type enzyme | Streptomyces viridochromogenes |
| S25D | catalytic turnover rate is 4fold less and Km is increased 460fold compared to the wild type enzyme | Streptomyces viridochromogenes |
| S25N | catalytic turnover rate is 1.5fold less and Km is increased 80fold compared to the wild type enzyme | Streptomyces viridochromogenes |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0032 | - |
3-phosphonopyruvate | wild type enzyme, at pH 8.0 (HEPES buffer) and at 30°C | Streptomyces viridochromogenes |  |
| 0.013 | - |
3-phosphonopyruvate | mutant enzyme H111A, at pH 8.0 (HEPES buffer) and at 30°C | Streptomyces viridochromogenes | |
| 0.04 | - |
3-phosphonopyruvate | mutant enzyme D297E, at pH 8.0 (HEPES buffer) and at 30°C | Streptomyces viridochromogenes | |
| 0.25 | - |
3-phosphonopyruvate | mutant enzyme S25N, at pH 8.0 (HEPES buffer) and at 30°C | Streptomyces viridochromogenes | |
| 1.47 | - |
3-phosphonopyruvate | mutant enzyme S25D, at pH 8.0 (HEPES buffer) and at 30°C | Streptomyces viridochromogenes | |
| 3.02 | - |
3-phosphonopyruvate | mutant enzyme D297N, at pH 8.0 (HEPES buffer) and at 30°C | Streptomyces viridochromogenes | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | Ca2+ can substitute for Mg2+ | Streptomyces viridochromogenes | |
| Mg2+ | Mg2+ is required for activity, Ca2+ and Mn2+ can substitute for Mg2+ | Streptomyces viridochromogenes | |
| Mn2+ | Mn2+ can substitute for Mg2+ | Streptomyces viridochromogenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43600 | - |
2 * 43600, gel filtration | Streptomyces viridochromogenes |
| 46000 | - |
2 * 46000, gel filtration | Streptomyces viridochromogenes |
| 72100 | - |
gel filtration | Streptomyces viridochromogenes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces viridochromogenes | O86938 | strain Tü494 | - |
| Streptomyces viridochromogenes DSM 40736 / JCM 4977 / BCRC 1201 / Tue 494 / Tu 494 | O86938 | strain Tü494 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Streptomyces viridochromogenes |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C and 4°C, half-life of 50 days | Streptomyces viridochromogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-phosphonopyruvate | - |
Streptomyces viridochromogenes | 2-phosphonoacetaldehyde + CO2 | - |
? | |
| 3-phosphonopyruvate | - |
Streptomyces viridochromogenes DSM 40736 / JCM 4977 / BCRC 1201 / Tue 494 / Tu 494 | 2-phosphonoacetaldehyde + CO2 | - |
? | |
| additional information | neither pyruvate, beta-hydroxypyruvate, nor fluoropyruvate serve as alternative substrates | Streptomyces viridochromogenes | ? | - |
? | |
| additional information | neither pyruvate, beta-hydroxypyruvate, nor fluoropyruvate serve as alternative substrates | Streptomyces viridochromogenes DSM 40736 / JCM 4977 / BCRC 1201 / Tue 494 / Tu 494 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 43600, gel filtration | Streptomyces viridochromogenes |
| homodimer | 2 * 46000, gel filtration | Streptomyces viridochromogenes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PPDC | - |
Streptomyces viridochromogenes |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.31 | - |
3-phosphonopyruvate | mutant enzyme S25D, at pH 8.0 (HEPES buffer) and at 30°C | Streptomyces viridochromogenes | |
| 0.55 | - |
3-phosphonopyruvate | mutant enzyme D297N, at pH 8.0 (HEPES buffer) and at 30°C | Streptomyces viridochromogenes | |
| 0.76 | - |
3-phosphonopyruvate | mutant enzyme D297E, at pH 8.0 (HEPES buffer) and at 30°C | Streptomyces viridochromogenes | |
| 0.78 | - |
3-phosphonopyruvate | mutant enzyme S25N, at pH 8.0 (HEPES buffer) and at 30°C | Streptomyces viridochromogenes | |
| 1.18 | - |
3-phosphonopyruvate | wild type enzyme, at pH 8.0 (HEPES buffer) and at 30°C | Streptomyces viridochromogenes | |
| 1.24 | - |
3-phosphonopyruvate | mutant enzyme H111A, at pH 8.0 (HEPES buffer) and at 30°C | Streptomyces viridochromogenes | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Streptomyces viridochromogenes |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | dependent on | Streptomyces viridochromogenes | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
