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Literature summary for 4.1.1.82 extracted from
- The phosphonopyruvate decarboxylase from Bacteroides fragilis (2003), J. Biol. Chem., 278, 41302-41308.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacteroides fragilis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D258A | 9% of wild-type kcat for 3-phosphonopyruvate | Bacteroides fragilis |
| D260A | 0.1% of wild-type kcat for 3-phosphonopyruvate | Bacteroides fragilis |
| E213A | 5% of wild-type kcat for 3-phosphonopyruvate | Bacteroides fragilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| sulfopyruvate | competitive inhibition | Bacteroides fragilis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0032 | - |
3-phosphonopyruvate | 25°C, pH 7.3, 5 mM MgCl2, 1 mM MnCl2, 0.2 mM thiamine pyrophosphate | Bacteroides fragilis | |
| 0.013 | - |
Mn2+ | 25°C, pH 7.3 | Bacteroides fragilis | |
| 0.013 | - |
thiamine diphosphate | 25°C, pH 7.3 | Bacteroides fragilis | |
| 0.078 | - |
Ca2+ | 25°C, pH 7.3 | Bacteroides fragilis | |
| 0.082 | - |
Mg2+ | 25°C, pH 7.3 | Bacteroides fragilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required for activity | Bacteroides fragilis | |
| Mg2+ | required for activity | Bacteroides fragilis | |
| Mn2+ | required for activity | Bacteroides fragilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
3 * 40000, SDS-PAGE | Bacteroides fragilis |
| 41199 | - |
3 * 41199, MALDI-TOF mass spectroscopy | Bacteroides fragilis |
| 120000 | - |
gel filtration | Bacteroides fragilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-phosphonopyruvate | Bacteroides fragilis | - |
2-phosphonoacetaldehyde + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacteroides fragilis | Q9F768 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant Ppyr decarboxylase, ammonium sulfate, DEAE-Sepahrose, hydroxylapatite | Bacteroides fragilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-phosphonopyruvate | - |
Bacteroides fragilis | 2-phosphonoacetaldehyde + CO2 | - |
? | |
| pyruvate | 0.5% of 3-phosphonopyruvate kcat | Bacteroides fragilis | acetaldehyde + CO2 | - |
? | |
| sulfopyruvate | 0.5% of 3-phosphonopyruvate kcat | Bacteroides fragilis | sulfoacetaldehyde + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 40000, SDS-PAGE | Bacteroides fragilis |
| trimer | 3 * 41199, MALDI-TOF mass spectroscopy | Bacteroides fragilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ppyr decarboxylase | - |
Bacteroides fragilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10.2 | - |
3-phosphonopyruvate | 25°C, pH 7.3, 5 mM MgCl2, 1 mM MnCl2, 0.2 mM thiamine diphosphate | Bacteroides fragilis | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | - |
Bacteroides fragilis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
sulfopyruvate | 25°C, pH 7.0 | Bacteroides fragilis | |
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