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Literature summary for 4.1.1.76 extracted from
- Engineering the promiscuous racemase activity of an arylmalonate decarboxylase (2011), Chemistry, 17, 557-563.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Top10 cells | Bordetella bronchiseptica |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G74C | the mutant of arylmalonate decarboxylase from Bordatella bronchoseptica has a racemising activity towards profens | Bordetella bronchiseptica |
| G74C/G190E | inactive | Bordetella bronchiseptica |
| G74C/G190H | inactive | Bordetella bronchiseptica |
| G74C/M159L | the mutant shows 14% decarboxylation activity and 165% racemisation activity compared to mutant G74C | Bordetella bronchiseptica |
| G74C/P14D | inactive | Bordetella bronchiseptica |
| G74C/V13D | inactive | Bordetella bronchiseptica |
| G74C/V13D/G190H | inactive | Bordetella bronchiseptica |
| G74C/V43A | the mutant with shows a 20fold shift towards promiscuous racemisation based on a reduced activity in the decarboxylation reaction and a 2fold increase in the racemisation activity. The mutant shows an extended substrate range, with a 30fold increase in the reaction rate towards ketoprofen | Bordetella bronchiseptica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10.4 | - |
2-aryl-2-methylmalonate | mutant enzyme G74C, pH and temperature not specified in the publication | Bordetella bronchiseptica |  |
| 11.4 | - |
2-aryl-2-methylmalonate | mutant enzyme G74C/V43A, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
| 18.3 | - |
2-aryl-2-methylmalonate | mutant enzyme G74C/M159L, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bordetella bronchiseptica | Q05115 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-Sepharose column chromatography, gel filtration | Bordetella bronchiseptica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-aryl-2-methylmalonate | - |
Bordetella bronchiseptica | 2-arylpropionate + CO2 | - |
? | |
| additional information | 2-methyl-2-(4-isobutylphenyl)malonic acid is not a substrate of wild type AMDase | Bordetella bronchiseptica | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AMDase | - |
Bordetella bronchiseptica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.022 | - |
2-aryl-2-methylmalonate | mutant enzyme G74C/V43A, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
| 0.063 | - |
2-aryl-2-methylmalonate | mutant enzyme G74C/M159L, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
| 0.27 | - |
2-aryl-2-methylmalonate | mutant enzyme G74C, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0019 | - |
2-aryl-2-methylmalonate | mutant enzyme G74C/V43A, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
| 0.0034 | - |
2-aryl-2-methylmalonate | mutant enzyme G74C/M159L, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
| 0.026 | - |
2-aryl-2-methylmalonate | mutant enzyme G74C, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
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