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Literature summary for 4.1.1.76 extracted from
- Purification and properties of a novel acylmalonate decarboxylase from Alcaligenes bronchisepticus KU 1201 (1992), Eur. J. Biochem., 210, 475-481.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| HgCl | - |
Bordetella bronchiseptica |  |
| HgCl2 | - |
Bordetella bronchiseptica | |
| NEM | - |
Bordetella bronchiseptica | |
| PbCl2 | - |
Bordetella bronchiseptica | |
| SnCl2 | - |
Bordetella bronchiseptica | |
| Sulfhydryl agents | - |
Bordetella bronchiseptica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.09 | - |
1-Naphthylmalonate | - |
Bordetella bronchiseptica | |
| 8.32 | - |
p-Chlorophenylmalonate | - |
Bordetella bronchiseptica | |
| 8.89 | - |
p-methylphenylmalonate | - |
Bordetella bronchiseptica | |
| 9.36 | - |
p-Methoxyphenylmalonate | - |
Bordetella bronchiseptica | |
| 9.84 | - |
m-Chlorophenylmalonate | - |
Bordetella bronchiseptica | |
| 12.5 | - |
alpha-Methyl-alpha(2-thienyl)malonate | - |
Bordetella bronchiseptica | |
| 12.6 | - |
o-Chlorophenylmalonate | - |
Bordetella bronchiseptica | |
| 13.5 | - |
p-Fluorophenylmalonate | - |
Bordetella bronchiseptica | |
| 13.9 | - |
Phenylmalonate | - |
Bordetella bronchiseptica | |
| 19.4 | - |
2-Naphthylmalonate | - |
Bordetella bronchiseptica | |
| 25.5 | - |
alpha-methyl-alpha-phenylmalonate | - |
Bordetella bronchiseptica | |
| 27.5 | - |
o-Fluorophenylmalonate | - |
Bordetella bronchiseptica | |
| 35.7 | - |
m-Fluorophenylmalonate | - |
Bordetella bronchiseptica | |
| 87.4 | - |
2-Thienylmalonate | - |
Bordetella bronchiseptica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
gel filtration | Bordetella bronchiseptica |
| 24000 | - |
1 * 24000, SDS-PAGE | Bordetella bronchiseptica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bordetella bronchiseptica | - |
KU 1201 | - |
| Bordetella bronchiseptica KU 1201 | - |
KU 1201 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bordetella bronchiseptica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 377 | - |
- |
Bordetella bronchiseptica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 6.5-8.5, stable for over 6 months | Bordetella bronchiseptica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-Naphthylmalonate | - |
Bordetella bronchiseptica | ? | - |
? | |
| 2-Naphthylmalonate | - |
Bordetella bronchiseptica | ? | - |
? | |
| 2-Thienylmalonate | - |
Bordetella bronchiseptica | ? | - |
? | |
| alpha-Methyl-alpha(2-thienyl)malonate | - |
Bordetella bronchiseptica | ? | - |
? | |
| alpha-Methyl-alpha-phenylmalonate | - |
Bordetella bronchiseptica | ? | - |
? | |
| alpha-Methyl-alpha-phenylmalonate | - |
Bordetella bronchiseptica KU 1201 | ? | - |
? | |
| m-Chlorophenylmalonate | - |
Bordetella bronchiseptica | ? | - |
? | |
| m-Fluorophenylmalonate | - |
Bordetella bronchiseptica | ? | - |
? | |
| o-Chlorophenylmalonate | - |
Bordetella bronchiseptica | ? | - |
? | |
| o-Fluorophenylmalonate | - |
Bordetella bronchiseptica | ? | - |
? | |
| o-Fluorophenylmalonate | - |
Bordetella bronchiseptica KU 1201 | ? | - |
? | |
| p-Chlorophenylmalonate | - |
Bordetella bronchiseptica | ? | - |
? | |
| p-Fluorophenylmalonate | - |
Bordetella bronchiseptica | ? | - |
? | |
| p-Fluorophenylmalonate | - |
Bordetella bronchiseptica KU 1201 | ? | - |
? | |
| p-Methoxyphenylmalonate | - |
Bordetella bronchiseptica | ? | - |
? | |
| p-Methoxyphenylmalonate | - |
Bordetella bronchiseptica KU 1201 | ? | - |
? | |
| p-Methylphenylmalonate | - |
Bordetella bronchiseptica | ? | - |
? | |
| p-Methylphenylmalonate | - |
Bordetella bronchiseptica KU 1201 | ? | - |
? | |
| Phenylmalonate | - |
Bordetella bronchiseptica | Phenylacetate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 24000, SDS-PAGE | Bordetella bronchiseptica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Bordetella bronchiseptica |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 55 | 45°C: maximal activity, 55°C: drastically reduced activity above, probably because of inactivation of the enzyme | Bordetella bronchiseptica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
below, 10 min, stable | Bordetella bronchiseptica |
| 40 | - |
10 min, about 10% loss of activity | Bordetella bronchiseptica |
| 50 | - |
10 min, about 50% loss of activity | Bordetella bronchiseptica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 29.8 | - |
alpha-methyl-alpha-phenylmalonate | - |
Bordetella bronchiseptica | |
| 92 | - |
p-Methoxyphenylmalonate | - |
Bordetella bronchiseptica | |
| 125 | - |
p-methylphenylmalonate | - |
Bordetella bronchiseptica | |
| 195 | - |
p-Fluorophenylmalonate | - |
Bordetella bronchiseptica | |
| 200 | - |
alpha-Methyl-alpha(2-thienyl)malonate | - |
Bordetella bronchiseptica | |
| 353 | - |
Phenylmalonate | - |
Bordetella bronchiseptica | |
| 576 | - |
1-Naphthylmalonate | - |
Bordetella bronchiseptica | |
| 666 | - |
o-Fluorophenylmalonate | - |
Bordetella bronchiseptica | |
| 782 | - |
p-Chlorophenylmalonate | - |
Bordetella bronchiseptica | |
| 1090 | - |
o-Chlorophenylmalonate | - |
Bordetella bronchiseptica | |
| 1420 | - |
m-Chlorophenylmalonate | - |
Bordetella bronchiseptica | |
| 1730 | - |
2-Naphthylmalonate | - |
Bordetella bronchiseptica | |
| 2180 | - |
m-Fluorophenylmalonate | - |
Bordetella bronchiseptica | |
| 5320 | - |
2-Thienylmalonate | - |
Bordetella bronchiseptica | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Bordetella bronchiseptica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | pH 6.0: about 55% of maximal activity, pH 9: about 80% of maximal activity | Bordetella bronchiseptica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
30°C, 30 min, about 60% loss of activity | Bordetella bronchiseptica |
| 6.5 | 8.5 | 4°C, stable for over 6 months | Bordetella bronchiseptica |
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