Any feedback?
Literature summary for 4.1.1.74 extracted from
- Intermediates and transition states in thiamin diphosphate-dependent decarboxylases. A kinetic and NMR study on wild-type indolepyruvate decarboxylase and variants using indolepyruvate, benzoylformate, and pyruvate as substrates (2005), Biochemistry, 44, 6164-6179.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D29E | significant decrease in Km-values | Enterobacter cloacae |
| E468D | slight decrease in Km-values | Enterobacter cloacae |
| E52D | decrease in Km-value for 3-(indol-3-yl)pyruvate and pyruvate, increase in Km-value for benzoylformic acid | Enterobacter cloacae |
| H115K | slight increase in Km-value for 3-(indol-3-yl)pyruvate and pyruvate, decrease in Km-value for benzoylformic acid | Enterobacter cloacae |
| Q383T | decrease in Km-value for pyruvate | Enterobacter cloacae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0045 | - |
3-(Indol-3-yl)pyruvate | mutant E52D, pH 6.5, 30°C | Enterobacter cloacae |  |
| 0.018 | - |
3-(Indol-3-yl)pyruvate | mutant E468D, pH 6.5, 30°C | Enterobacter cloacae | |
| 0.02 | - |
3-(Indol-3-yl)pyruvate | wild-type, pH 6.5, 30°C | Enterobacter cloacae | |
| 0.024 | - |
benzoylformic acid | mutant D29E, pH 6.5, 30°C | Enterobacter cloacae | |
| 0.024 | - |
3-(Indol-3-yl)pyruvate | mutant Q383T, pH 6.5, 30°C | Enterobacter cloacae | |
| 0.032 | - |
3-(Indol-3-yl)pyruvate | mutant H115K, pH 6.5, 30°C | Enterobacter cloacae | |
| 0.14 | - |
benzoylformic acid | mutant E468D, pH 6.5, 30°C | Enterobacter cloacae | |
| 0.27 | - |
benzoylformic acid | mutant H115K, pH 6.5, 30°C | Enterobacter cloacae | |
| 0.63 | - |
pyruvate | mutant D29E, pH 6.5, 30°C | Enterobacter cloacae | |
| 0.73 | - |
pyruvate | mutant Q383T, pH 6.5, 30°C | Enterobacter cloacae | |
| 0.77 | - |
pyruvate | mutant E52D, pH 6.5, 30°C | Enterobacter cloacae | |
| 1.65 | - |
benzoylformic acid | wild-type, pH 6.5, 30°C | Enterobacter cloacae | |
| 1.71 | - |
benzoylformic acid | mutant Q383T, pH 6.5, 30°C | Enterobacter cloacae | |
| 2.72 | - |
benzoylformic acid | mutant E52D, pH 6.5, 30°C | Enterobacter cloacae | |
| 3.16 | - |
pyruvate | mutant E468D, pH 6.5, 30°C | Enterobacter cloacae | |
| 3.38 | - |
pyruvate | wild-type, pH 6.5, 30°C | Enterobacter cloacae | |
| 3.48 | - |
pyruvate | mutant H115K, pH 6.5, 30°C | Enterobacter cloacae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterobacter cloacae | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 3-(indol-3-yl)pyruvate = 2-(indol-3-yl)acetaldehyde + CO2 | residues E468, D29, and H115 are involved in binding of all substrates and decarboxlation of the respective tetrahedral thiamine-diphosphate-substrate adducts. Residue E383 is central to substrate specificity | Enterobacter cloacae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-(indol-3-yl)pyruvate | - |
Enterobacter cloacae | 2-(indol-3-yl)acetaldehyde + CO2 | - |
? | |
| benzoylformic acid | - |
Enterobacter cloacae | benzaldehyde + CO2 | - |
? | |
| pyruvate | - |
Enterobacter cloacae | acetaldehyde + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ipyr | - |
Enterobacter cloacae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
benzoylformic acid | mutant D29E, pH 6.5, 30°C | Enterobacter cloacae | |
| 0.016 | - |
pyruvate | mutant H115K, pH 6.5, 30°C | Enterobacter cloacae | |
| 0.019 | - |
pyruvate | mutant D29E, pH 6.5, 30°C | Enterobacter cloacae | |
| 0.04 | - |
3-(Indol-3-yl)pyruvate | mutant E468D, pH 6.5, 30°C | Enterobacter cloacae | |
| 0.06 | - |
pyruvate | mutant E52D, pH 6.5, 30°C | Enterobacter cloacae | |
| 0.069 | - |
pyruvate | mutant E468D, pH 6.5, 30°C | Enterobacter cloacae | |
| 0.07 | - |
benzoylformic acid | mutant E468D, pH 6.5, 30°C | Enterobacter cloacae | |
| 0.1 | - |
3-(Indol-3-yl)pyruvate | mutant E52D, pH 6.5, 30°C | Enterobacter cloacae | |
| 0.29 | - |
benzoylformic acid | mutant H115K, pH 6.5, 30°C | Enterobacter cloacae | |
| 0.31 | - |
3-(Indol-3-yl)pyruvate | mutant H115K, pH 6.5, 30°C | Enterobacter cloacae | |
| 0.87 | - |
benzoylformic acid | mutant E52D, pH 6.5, 30°C | Enterobacter cloacae | |
| 0.88 | - |
pyruvate | wild-type, pH 6.5, 30°C | Enterobacter cloacae | |
| 0.98 | - |
3-(Indol-3-yl)pyruvate | wild-type, pH 6.5, 30°C | Enterobacter cloacae | |
| 2.05 | - |
3-(Indol-3-yl)pyruvate | mutant Q383T, pH 6.5, 30°C | Enterobacter cloacae | |
| 2.06 | - |
pyruvate | mutant Q383T, pH 6.5, 30°C | Enterobacter cloacae | |
| 2.25 | - |
benzoylformic acid | mutant Q383T, pH 6.5, 30°C | Enterobacter cloacae | |
| 11.6 | - |
benzoylformic acid | wild-type, pH 6.5, 30°C | Enterobacter cloacae | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
