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Literature summary for 4.1.1.74 extracted from

  • Wakagi, T.; Fukuda, E.; Ogawa, Y.; Kino, H.; Matsuzawa, H.
    A novel bifunctional molybdo-enzyme catalyzing both decarboxylation of indolepyruvate and oxidation of indoleacetaldehyde from a thermoacidophilic archaeon, Sulfolobus sp. strain 7 (2002), FEBS Lett., 510, 196-200.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
Indole-3-acetaldehyde 70°C, pH 8.0 Sulfolobus sp.
0.042
-
glyceraldehyde 70°C, pH 8.0 Sulfolobus sp.
0.105
-
phenylacetaldehyde 70°C, pH 8.0 Sulfolobus sp.
0.395
-
benzaldehyde 70°C, pH 8.0 Sulfolobus sp.
0.788
-
Indole-3-pyruvate 70°C, pH 8.0 Sulfolobus sp.
1.66
-
phenylpyruvate 70°C, pH 8.0 Sulfolobus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 8.7 mol per mol of enzyme Sulfolobus sp.
Mo2+ 0.8 mol per mol of enzyme Sulfolobus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
2 * 89000, alpha, + 2 * 30000, beta, + 2 * 19000, gamma, SDS-PAGE Sulfolobus sp.
30000
-
2 * 89000, alpha, + 2 * 30000, beta, + 2 * 19000, gamma, SDS-PAGE Sulfolobus sp.
89000
-
2 * 89000, alpha, + 2 * 30000, beta, + 2 * 19000, gamma, SDS-PAGE Sulfolobus sp.
280000
-
gel filtration Sulfolobus sp.

Organism

Organism UniProt Comment Textmining
Sulfolobus sp.
-
-
-
Sulfolobus sp. 7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfolobus sp.

Reaction

Reaction Comment Organism Reaction ID
3-(indol-3-yl)pyruvate = 2-(indol-3-yl)acetaldehyde + CO2 mechanism Sulfolobus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate
-
Sulfolobus sp. ?
-
?
2-oxoglutarate
-
Sulfolobus sp. 7 ?
-
?
2-oxoisovalerate
-
Sulfolobus sp. ?
-
?
2-oxoisovalerate
-
Sulfolobus sp. 7 ?
-
?
3-(indol-3-yl)pyruvate
-
Sulfolobus sp. 2-(indol-3-yl)acetaldehyde + CO2
-
?
3-(indol-3-yl)pyruvate
-
Sulfolobus sp. 7 2-(indol-3-yl)acetaldehyde + CO2
-
?
benzaldehyde
-
Sulfolobus sp. ?
-
?
glyceraldehyde
-
Sulfolobus sp. ?
-
?
indole-3-acetaldehyde
-
Sulfolobus sp. ?
-
?
indole-3-acetaldehyde
-
Sulfolobus sp. 7 ?
-
?
phenylacetaldehyde
-
Sulfolobus sp. ?
-
?
phenylpyruvate
-
Sulfolobus sp. phenylethanal + CO2
-
?
pyruvate
-
Sulfolobus sp. acetaldehyde + CO2
-
?
pyruvate
-
Sulfolobus sp. 7 acetaldehyde + CO2
-
?

Subunits

Subunits Comment Organism
hexamer 2 * 89000, alpha, + 2 * 30000, beta, + 2 * 19000, gamma, SDS-PAGE Sulfolobus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
30 min, pH 7.0, almost stable Sulfolobus sp.
90
-
30 min, 22% inactivation Sulfolobus sp.
100
-
30 min, 97% inactivation Sulfolobus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
70% loss of activity at pH 7.0, 15% loss of activity at pH 9 Sulfolobus sp.

Cofactor

Cofactor Comment Organism Structure
biotin binds possibly to beta subunit Sulfolobus sp.
FMN 1.9 mol per mol of enzyme Sulfolobus sp.
additional information no thiamine diphosphate Sulfolobus sp.