KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
Indole-3-acetaldehyde | 70°C, pH 8.0 | Sulfolobus sp. | |
0.042 | - |
glyceraldehyde | 70°C, pH 8.0 | Sulfolobus sp. | |
0.105 | - |
phenylacetaldehyde | 70°C, pH 8.0 | Sulfolobus sp. | |
0.395 | - |
benzaldehyde | 70°C, pH 8.0 | Sulfolobus sp. | |
0.788 | - |
Indole-3-pyruvate | 70°C, pH 8.0 | Sulfolobus sp. | |
1.66 | - |
phenylpyruvate | 70°C, pH 8.0 | Sulfolobus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 8.7 mol per mol of enzyme | Sulfolobus sp. | |
Mo2+ | 0.8 mol per mol of enzyme | Sulfolobus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
2 * 89000, alpha, + 2 * 30000, beta, + 2 * 19000, gamma, SDS-PAGE | Sulfolobus sp. |
30000 | - |
2 * 89000, alpha, + 2 * 30000, beta, + 2 * 19000, gamma, SDS-PAGE | Sulfolobus sp. |
89000 | - |
2 * 89000, alpha, + 2 * 30000, beta, + 2 * 19000, gamma, SDS-PAGE | Sulfolobus sp. |
280000 | - |
gel filtration | Sulfolobus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus sp. | - |
- |
- |
Sulfolobus sp. 7 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfolobus sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3-(indol-3-yl)pyruvate = 2-(indol-3-yl)acetaldehyde + CO2 | mechanism | Sulfolobus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate | - |
Sulfolobus sp. | ? | - |
? | |
2-oxoglutarate | - |
Sulfolobus sp. 7 | ? | - |
? | |
2-oxoisovalerate | - |
Sulfolobus sp. | ? | - |
? | |
2-oxoisovalerate | - |
Sulfolobus sp. 7 | ? | - |
? | |
3-(indol-3-yl)pyruvate | - |
Sulfolobus sp. | 2-(indol-3-yl)acetaldehyde + CO2 | - |
? | |
3-(indol-3-yl)pyruvate | - |
Sulfolobus sp. 7 | 2-(indol-3-yl)acetaldehyde + CO2 | - |
? | |
benzaldehyde | - |
Sulfolobus sp. | ? | - |
? | |
glyceraldehyde | - |
Sulfolobus sp. | ? | - |
? | |
indole-3-acetaldehyde | - |
Sulfolobus sp. | ? | - |
? | |
indole-3-acetaldehyde | - |
Sulfolobus sp. 7 | ? | - |
? | |
phenylacetaldehyde | - |
Sulfolobus sp. | ? | - |
? | |
phenylpyruvate | - |
Sulfolobus sp. | phenylethanal + CO2 | - |
? | |
pyruvate | - |
Sulfolobus sp. | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Sulfolobus sp. 7 | acetaldehyde + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 2 * 89000, alpha, + 2 * 30000, beta, + 2 * 19000, gamma, SDS-PAGE | Sulfolobus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
30 min, pH 7.0, almost stable | Sulfolobus sp. |
90 | - |
30 min, 22% inactivation | Sulfolobus sp. |
100 | - |
30 min, 97% inactivation | Sulfolobus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
70% loss of activity at pH 7.0, 15% loss of activity at pH 9 | Sulfolobus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
biotin | binds possibly to beta subunit | Sulfolobus sp. | |
FMN | 1.9 mol per mol of enzyme | Sulfolobus sp. | |
additional information | no thiamine diphosphate | Sulfolobus sp. |