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Literature summary for 4.1.1.74 extracted from
- Studies on structure-function relationships of indolepyruvate decarboxylase from Enterobacter cloacae, a key enzyme of the indole acetic acid pathway (2003), Eur. J. Biochem., 270, 2322-2331.
General Stability
| General Stability | Organism |
|---|---|
| ammonium sulfate stabilizes | Enterobacter cloacae |
| thiamine diphosphate stabilizes | Enterobacter cloacae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
4-nitro-benzoylformic acid | pH 6.5, 30°C | Enterobacter cloacae |  |
| 0.019 | - |
4-bromo-benzoylformic acid | pH 6.5, 30°C | Enterobacter cloacae | |
| 0.02 | - |
Indole-3-pyruvate | pH 6.5, 30°C | Enterobacter cloacae | |
| 0.048 | - |
4-chloro-benzoylformic acid | pH 6.5, 30°C | Enterobacter cloacae | |
| 0.111 | - |
4-ethyl-benzoylformic acid | pH 6.5, 30°C | Enterobacter cloacae | |
| 0.127 | - |
4-methyl-benzoylformic acid | pH 6.5, 30°C | Enterobacter cloacae | |
| 0.62 | - |
4-fluoro-benzoylformic acid | pH 6.5, 30°C | Enterobacter cloacae | |
| 1.05 | - |
4-methoxy-benzoylformic acid | pH 6.5, 30°C | Enterobacter cloacae | |
| 1.65 | - |
benzoylformic acid | pH 6.5, 30°C | Enterobacter cloacae | |
| 3.38 | - |
pyruvate | pH 6.5, 30°C | Enterobacter cloacae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Enterobacter cloacae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
4 * 60000, SDS-PAGE | Enterobacter cloacae |
| 245000 | - |
gel filtration | Enterobacter cloacae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterobacter cloacae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli | Enterobacter cloacae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 3-(indol-3-yl)pyruvate = 2-(indol-3-yl)acetaldehyde + CO2 | mechanism | Enterobacter cloacae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| quite stable at 40°C without further additions | Enterobacter cloacae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-(indol-3-yl)pyruvate | - |
Enterobacter cloacae | 2-(indol-3-yl)acetaldehyde + CO2 | - |
? | |
| 4-bromo-benzoylformic acid | - |
Enterobacter cloacae | 4-bromobenzaldehyde + CO2 | - |
? | |
| 4-chloro-benzoylformic acid | - |
Enterobacter cloacae | 4-chlorobenzaldehyde + CO2 | - |
? | |
| 4-ethyl-benzoylformic acid | - |
Enterobacter cloacae | 4-ethylbenzaldehyde + CO2 | - |
? | |
| 4-fluoro-benzoylformic acid | - |
Enterobacter cloacae | 4-fluorobenzaldehyde + CO2 | - |
? | |
| 4-methoxy-benzoylformic acid | - |
Enterobacter cloacae | 4-methoxybenzaldehyde + CO2 | - |
? | |
| 4-methyl-benzoylformic acid | - |
Enterobacter cloacae | 4-methylbenzaldehyde + CO2 | - |
? | |
| 4-nitro-benzoylformic acid | - |
Enterobacter cloacae | 4-nitrobenzaldehyde + CO2 | - |
? | |
| benzoylformic acid | - |
Enterobacter cloacae | benzaldehyde + CO2 | - |
? | |
| pyruvate | - |
Enterobacter cloacae | acetaldehyde + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | at pH-values between pH 5.6 and 6.0 the tetrameric form dominates, at pH values above 8.0, the monomeric form is present | Enterobacter cloacae |
| tetramer | 4 * 60000, SDS-PAGE | Enterobacter cloacae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | - |
Enterobacter cloacae | |
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