BRENDA - Enzyme Database show
show all sequences of 4.1.1.73

Purification and characterization of a new NAD(+)-dependent enzyme, L-tartrate decarboxylase, from Pseudomonas sp. group Ve-2

Furuyoshi, S.; Nawa, Y.; Kawabata, N.; Tanaka, H.; Soda, K.; J. Biochem. 110, 520-525 (1991)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
D-glycerate
product inhibition, 10 mM, 65% inhibition
Pseudomonas sp.
D-malate
10 mM, 83% inhibition
Pseudomonas sp.
EDTA
5 mM, at 30C, complete inactivation
Pseudomonas sp.
L-Glycerate
10 mM, 63% inhibition
Pseudomonas sp.
L-malate
10 mM, 29% inhibition
Pseudomonas sp.
meso-tartrate
10 mM, 14% inhibition
Pseudomonas sp.
succinate
10 mM, 28% inhibition
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1
-
NAD+
pH 7.5, 30C
Pseudomonas sp.
7
-
L-Tartrate
pH 7.5, 30C
Pseudomonas sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
activation
Pseudomonas sp.
Cu2+
slight activation
Pseudomonas sp.
K+
activation, above 40 mM
Pseudomonas sp.
Mg2+
above 0.2 mM; activation
Pseudomonas sp.
Mn2+
activation
Pseudomonas sp.
additional information
requirement of a divalent and a monovalent cation, not activated by Ca2+, Fe2+, Zn2+, Li+ or Na+
Pseudomonas sp.
NH4+
slight activation
Pseudomonas sp.
Ni2+
slight activation
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
4 * 40000, SDS-PAGE
Pseudomonas sp.
148000
-
HPLC gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-tartrate
Pseudomonas sp.
inducible by L-tartrate
D-glycerate + CO2
-
-
?
L-tartrate
Pseudomonas sp. 5D1A
inducible by L-tartrate
D-glycerate + CO2
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
group Ve-2; soil isolate
-
Pseudomonas sp. 5D1A
-
group Ve-2
-
Purification (Commentary)
Commentary
Organism
about 11fold
Pseudomonas sp.
Reaction
Reaction
Commentary
Organism
(R,R)-tartrate = D-glycerate + CO2
reduction proceeds concomitantly with decarboxylation, but at a much lower rate
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.8
-
pH 7.5, 30C
Pseudomonas sp.
Storage Stability
Storage Stability
Organism
-20C, 50% glycerol, 3 months, stable, without glycerol, 1 month, 40% loss of activity
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-malate + NAD+
-
648380
Pseudomonas sp.
pyruvate + CO2 + NADH
-
648380
Pseudomonas sp.
?
D-malate + NAD+
-
648380
Pseudomonas sp. 5D1A
pyruvate + CO2 + NADH
-
648380
Pseudomonas sp. 5D1A
?
L-tartrate
best substrate
648380
Pseudomonas sp.
D-glycerate + CO2
-
648380
Pseudomonas sp.
?
L-tartrate
inducible by L-tartrate
648380
Pseudomonas sp.
D-glycerate + CO2
-
-
-
?
L-tartrate
best substrate
648380
Pseudomonas sp. 5D1A
D-glycerate + CO2
-
648380
Pseudomonas sp. 5D1A
?
L-tartrate
inducible by L-tartrate
648380
Pseudomonas sp. 5D1A
D-glycerate + CO2
-
-
-
?
meso-tartrate
-
648380
Pseudomonas sp.
D-glycerate + CO2
-
648380
Pseudomonas sp.
?
additional information
not: dihydroxyfumarate
648380
Pseudomonas sp.
?
-
-
-
-
additional information
enzyme resembles L-tartrate dehydrogenase/D-malate dehydrogenase from Rhodopseudomonas sphaeroides and tartrate dehydrogenase from Pseudomonas putida, substrate specificity
648380
Pseudomonas sp.
?
-
-
-
-
additional information
not: dihydroxyfumarate
648380
Pseudomonas sp. 5D1A
?
-
-
-
-
additional information
enzyme resembles L-tartrate dehydrogenase/D-malate dehydrogenase from Rhodopseudomonas sphaeroides and tartrate dehydrogenase from Pseudomonas putida, substrate specificity
648380
Pseudomonas sp. 5D1A
?
-
-
-
-
oxaloacetate + NAD+
-
648380
Pseudomonas sp.
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 40000, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Pseudomonas sp.
40
-
-
Pseudomonas sp.
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
50
-
30 min, in 20 mM Tris-HCl buffer, pH 7.5, 0.2 mM MgCl2, stable
Pseudomonas sp.
60
-
30 min, in 20 mM Tris-HCl buffer, pH 7.5, 0.2 mM MgCl2, 5% loss of activity, inactivation at pH 6.5 and 9.5
Pseudomonas sp.
65
-
30 min, in 20 mM Tris-HCl buffer, pH 7.5, 0.2 mM MgCl2, 29% loss of activity
Pseudomonas sp.
70
-
30 min, in 20 mM Tris-HCl buffer, pH 7.5, 0.2 mM MgCl2, inactivation
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8
-
Pseudomonas sp.
7.5
-
assay at
Pseudomonas sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
9
half-maximal activity at pH 5.5 and 9
Pseudomonas sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
-
30 min at 60C, inactivation
Pseudomonas sp.
7
-
30 min at 60C, 23% loss of activity
Pseudomonas sp.
7.5
-
30 min at 60C, in 20 mM Tris-HCl buffer, 0.2 mM MgCl2, 5% loss of activity
Pseudomonas sp.
8
-
30 min at 60C, 13% loss of activity
Pseudomonas sp.
9
-
30 min at 60C, 57% loss of activity
Pseudomonas sp.
9.5
-
30 min at 60C, inactivation
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
decarboxylation is not stoichiometrically related with NAD+, NAD+-reduction at 1.1% the rate of L-tartrate decarboxylation; requirement
Pseudomonas sp.
NADP+
requirement, less effective than NAD+
Pseudomonas sp.
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Pseudomonas sp.
isoelectric focusing
-
4.9
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
decarboxylation is not stoichiometrically related with NAD+, NAD+-reduction at 1.1% the rate of L-tartrate decarboxylation; requirement
Pseudomonas sp.
NADP+
requirement, less effective than NAD+
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
D-glycerate
product inhibition, 10 mM, 65% inhibition
Pseudomonas sp.
D-malate
10 mM, 83% inhibition
Pseudomonas sp.
EDTA
5 mM, at 30C, complete inactivation
Pseudomonas sp.
L-Glycerate
10 mM, 63% inhibition
Pseudomonas sp.
L-malate
10 mM, 29% inhibition
Pseudomonas sp.
meso-tartrate
10 mM, 14% inhibition
Pseudomonas sp.
succinate
10 mM, 28% inhibition
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1
-
NAD+
pH 7.5, 30C
Pseudomonas sp.
7
-
L-Tartrate
pH 7.5, 30C
Pseudomonas sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
activation
Pseudomonas sp.
Cu2+
slight activation
Pseudomonas sp.
K+
activation, above 40 mM
Pseudomonas sp.
Mg2+
above 0.2 mM; activation
Pseudomonas sp.
Mn2+
activation
Pseudomonas sp.
additional information
requirement of a divalent and a monovalent cation, not activated by Ca2+, Fe2+, Zn2+, Li+ or Na+
Pseudomonas sp.
NH4+
slight activation
Pseudomonas sp.
Ni2+
slight activation
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
4 * 40000, SDS-PAGE
Pseudomonas sp.
148000
-
HPLC gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-tartrate
Pseudomonas sp.
inducible by L-tartrate
D-glycerate + CO2
-
-
?
L-tartrate
Pseudomonas sp. 5D1A
inducible by L-tartrate
D-glycerate + CO2
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
about 11fold
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.8
-
pH 7.5, 30C
Pseudomonas sp.
Storage Stability (protein specific)
Storage Stability
Organism
-20C, 50% glycerol, 3 months, stable, without glycerol, 1 month, 40% loss of activity
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-malate + NAD+
-
648380
Pseudomonas sp.
pyruvate + CO2 + NADH
-
648380
Pseudomonas sp.
?
D-malate + NAD+
-
648380
Pseudomonas sp. 5D1A
pyruvate + CO2 + NADH
-
648380
Pseudomonas sp. 5D1A
?
L-tartrate
best substrate
648380
Pseudomonas sp.
D-glycerate + CO2
-
648380
Pseudomonas sp.
?
L-tartrate
inducible by L-tartrate
648380
Pseudomonas sp.
D-glycerate + CO2
-
-
-
?
L-tartrate
best substrate
648380
Pseudomonas sp. 5D1A
D-glycerate + CO2
-
648380
Pseudomonas sp. 5D1A
?
L-tartrate
inducible by L-tartrate
648380
Pseudomonas sp. 5D1A
D-glycerate + CO2
-
-
-
?
meso-tartrate
-
648380
Pseudomonas sp.
D-glycerate + CO2
-
648380
Pseudomonas sp.
?
additional information
not: dihydroxyfumarate
648380
Pseudomonas sp.
?
-
-
-
-
additional information
enzyme resembles L-tartrate dehydrogenase/D-malate dehydrogenase from Rhodopseudomonas sphaeroides and tartrate dehydrogenase from Pseudomonas putida, substrate specificity
648380
Pseudomonas sp.
?
-
-
-
-
additional information
not: dihydroxyfumarate
648380
Pseudomonas sp. 5D1A
?
-
-
-
-
additional information
enzyme resembles L-tartrate dehydrogenase/D-malate dehydrogenase from Rhodopseudomonas sphaeroides and tartrate dehydrogenase from Pseudomonas putida, substrate specificity
648380
Pseudomonas sp. 5D1A
?
-
-
-
-
oxaloacetate + NAD+
-
648380
Pseudomonas sp.
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 40000, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Pseudomonas sp.
40
-
-
Pseudomonas sp.
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
50
-
30 min, in 20 mM Tris-HCl buffer, pH 7.5, 0.2 mM MgCl2, stable
Pseudomonas sp.
60
-
30 min, in 20 mM Tris-HCl buffer, pH 7.5, 0.2 mM MgCl2, 5% loss of activity, inactivation at pH 6.5 and 9.5
Pseudomonas sp.
65
-
30 min, in 20 mM Tris-HCl buffer, pH 7.5, 0.2 mM MgCl2, 29% loss of activity
Pseudomonas sp.
70
-
30 min, in 20 mM Tris-HCl buffer, pH 7.5, 0.2 mM MgCl2, inactivation
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8
-
Pseudomonas sp.
7.5
-
assay at
Pseudomonas sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
9
half-maximal activity at pH 5.5 and 9
Pseudomonas sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
-
30 min at 60C, inactivation
Pseudomonas sp.
7
-
30 min at 60C, 23% loss of activity
Pseudomonas sp.
7.5
-
30 min at 60C, in 20 mM Tris-HCl buffer, 0.2 mM MgCl2, 5% loss of activity
Pseudomonas sp.
8
-
30 min at 60C, 13% loss of activity
Pseudomonas sp.
9
-
30 min at 60C, 57% loss of activity
Pseudomonas sp.
9.5
-
30 min at 60C, inactivation
Pseudomonas sp.
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Pseudomonas sp.
isoelectric focusing
-
4.9
Other publictions for EC 4.1.1.73
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
648379
Furuyoshi
-
D-Glycerate production from L- ...
Pseudomonas sp., Pseudomonas sp. 5D1A
Agric. Biol. Chem.
55
1515-1519
1991
-
1
-
-
-
-
1
-
-
1
-
2
-
4
-
-
-
-
-
-
-
-
4
-
2
-
-
-
2
-
-
1
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
648380
Furuyoshi
Purification and characterizat ...
Pseudomonas sp., Pseudomonas sp. 5D1A
J. Biochem.
110
520-525
1991
-
-
-
-
-
-
7
2
-
8
2
2
-
4
-
-
1
1
-
-
1
1
12
1
2
-
4
-
2
1
6
2
-
1
-
-
-
-
2
-
-
-
-
7
-
2
-
8
2
2
-
-
-
1
-
-
1
1
12
1
2
-
4
-
2
1
6
1
-
-
-
-
-
-
648378
Furuyoshi
-
Enzymic production of D-glycer ...
Pseudomonas sp., Pseudomonas sp. 5D1A
Agric. Biol. Chem.
53
2101-2105
1989
-
1
-
-
-
-
6
-
-
3
-
2
-
4
-
-
-
-
-
-
-
-
11
-
1
-
-
-
2
-
-
1
-
-
-
-
1
-
1
-
-
-
-
6
-
-
-
3
-
2
-
-
-
-
-
-
-
-
11
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-