Protein Variants | Comment | Organism |
---|---|---|
V461I | 3.3 degrees increase in thermostability compared to wild-type | Lactococcus lactis |
V461I/A290M | increase in thermostability compared to wild-type | Lactococcus lactis |
V461I/A290M/Q252N/D306G/E316R/F388Y | increase in thermostability compared to wild-type | Lactococcus lactis |
V461I/A290M/Q252N/D306G/E316R/F388Y/I404C | increase in thermostability compared to wild-type | Lactococcus lactis |
V461I/A290M/Q252N/D306G/E316R/F388Y/L434C | increase in thermostability compared to wild-type | Lactococcus lactis |
V461I/A290M/Q252N/D306G/E316R/F388Y/L434C/M290C | greater than 400fold improvement of half-life at 70°C and greater than 600fold increase in process stability in the presence of 4% isobutanol at 50°C | Lactococcus lactis |
V461I/A290M/Q252N/D306G/E316R/F388Y/L434C/M290V | increase in thermostability compared to wild-type | Lactococcus lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
3-methyl-2-oxobutanoate | mutant V461I/A290M/Q252N/D306G/E316R/F388Y/I404C, pH 6.5, 30°C | Lactococcus lactis | |
5.1 | - |
3-methyl-2-oxobutanoate | wild-type, pH 6.5, 30°C | Lactococcus lactis | |
5.2 | - |
3-methyl-2-oxobutanoate | mutant V461I/A290M/Q252N/D306G/E316R/F388Y/L434C/M290C, pH 6.5, 30°C | Lactococcus lactis | |
5.6 | - |
3-methyl-2-oxobutanoate | mutant V461I, pH 6.5, 30°C | Lactococcus lactis | |
6.6 | - |
3-methyl-2-oxobutanoate | mutant V461I/A290M/Q252N/D306G/E316R/F388Y/L434C/M290V, pH 6.5, 30°C | Lactococcus lactis | |
8.6 | - |
3-methyl-2-oxobutanoate | mutant A290M/Q252N/D306G/E316R/F388Y/L434C, pH 6.5, 30°C | Lactococcus lactis | |
9.3 | - |
3-methyl-2-oxobutanoate | mutant V461I/A290M, pH 6.5, 30°C | Lactococcus lactis | |
14.6 | - |
3-methyl-2-oxobutanoate | mutant V461I/A290M/Q252N/D306G/E316R/F388Y, pH 6.5, 30°C | Lactococcus lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | Q6QBS4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methyl-2-oxobutanoate | - |
Lactococcus lactis | 2-methylpropanal + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KdcA | - |
Lactococcus lactis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
61 | - |
wild-type, melting temperature | Lactococcus lactis |
64 | - |
mutant V461I, melting temperature | Lactococcus lactis |
66.5 | - |
mutant V461I/A290M, melting temperature | Lactococcus lactis |
70.5 | - |
mutant V461I/A290M/Q252N/D306G/E316R/F388Y, melting temperature | Lactococcus lactis |
74.5 | - |
mutant V461I/A290M/Q252N/D306G/E316R/F388Y/L434C, melting temperature | Lactococcus lactis |
74.5 | - |
mutant V461I/A290M/Q252N/D306G/E316R/F388Y/L434C/M290C, melting temperature | Lactococcus lactis |
74.5 | - |
mutant V461I/A290M/Q252N/D306G/E316R/F388Y/L434C/M290V, melting temperature | Lactococcus lactis |
75.5 | - |
mutant V461I/A290M/Q252N/D306G/E316R/F388Y/I404C, melting temperature | Lactococcus lactis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.2 | - |
3-methyl-2-oxobutanoate | mutant V461I/A290M/Q252N/D306G/E316R/F388Y/I404C, pH 6.5, 30°C | Lactococcus lactis | |
43 | - |
3-methyl-2-oxobutanoate | mutant V461I/A290M/Q252N/D306G/E316R/F388Y/L434C/M290V, pH 6.5, 30°C | Lactococcus lactis | |
69 | - |
3-methyl-2-oxobutanoate | mutant V461I/A290M/Q252N/D306G/E316R/F388Y/L434C/M290C, pH 6.5, 30°C | Lactococcus lactis | |
95 | - |
3-methyl-2-oxobutanoate | mutant A290M/Q252N/D306G/E316R/F388Y/L434C, pH 6.5, 30°C | Lactococcus lactis | |
118 | - |
3-methyl-2-oxobutanoate | mutant V461I/A290M, pH 6.5, 30°C | Lactococcus lactis | |
120 | - |
3-methyl-2-oxobutanoate | mutant V461I, pH 6.5, 30°C | Lactococcus lactis | |
145 | - |
3-methyl-2-oxobutanoate | wild-type, pH 6.5, 30°C | Lactococcus lactis | |
149 | - |
3-methyl-2-oxobutanoate | mutant V461I/A290M/Q252N/D306G/E316R/F388Y, pH 6.5, 30°C | Lactococcus lactis |