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Literature summary for 4.1.1.72 extracted from

  • Sutiono, S.; Carsten, J.; Sieber, V.
    Structure-guided engineering of alpha-keto acid decarboxylase for the production of higher alcohols at elevated temperature (2018), ChemSusChem, 11, 3335-3344 .
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
V461I 3.3 degrees increase in thermostability compared to wild-type Lactococcus lactis
V461I/A290M increase in thermostability compared to wild-type Lactococcus lactis
V461I/A290M/Q252N/D306G/E316R/F388Y increase in thermostability compared to wild-type Lactococcus lactis
V461I/A290M/Q252N/D306G/E316R/F388Y/I404C increase in thermostability compared to wild-type Lactococcus lactis
V461I/A290M/Q252N/D306G/E316R/F388Y/L434C increase in thermostability compared to wild-type Lactococcus lactis
V461I/A290M/Q252N/D306G/E316R/F388Y/L434C/M290C greater than 400fold improvement of half-life at 70°C and greater than 600fold increase in process stability in the presence of 4% isobutanol at 50°C Lactococcus lactis
V461I/A290M/Q252N/D306G/E316R/F388Y/L434C/M290V increase in thermostability compared to wild-type Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6
-
3-methyl-2-oxobutanoate mutant V461I/A290M/Q252N/D306G/E316R/F388Y/I404C, pH 6.5, 30°C Lactococcus lactis
5.1
-
3-methyl-2-oxobutanoate wild-type, pH 6.5, 30°C Lactococcus lactis
5.2
-
3-methyl-2-oxobutanoate mutant V461I/A290M/Q252N/D306G/E316R/F388Y/L434C/M290C, pH 6.5, 30°C Lactococcus lactis
5.6
-
3-methyl-2-oxobutanoate mutant V461I, pH 6.5, 30°C Lactococcus lactis
6.6
-
3-methyl-2-oxobutanoate mutant V461I/A290M/Q252N/D306G/E316R/F388Y/L434C/M290V, pH 6.5, 30°C Lactococcus lactis
8.6
-
3-methyl-2-oxobutanoate mutant A290M/Q252N/D306G/E316R/F388Y/L434C, pH 6.5, 30°C Lactococcus lactis
9.3
-
3-methyl-2-oxobutanoate mutant V461I/A290M, pH 6.5, 30°C Lactococcus lactis
14.6
-
3-methyl-2-oxobutanoate mutant V461I/A290M/Q252N/D306G/E316R/F388Y, pH 6.5, 30°C Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis Q6QBS4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methyl-2-oxobutanoate
-
Lactococcus lactis 2-methylpropanal + CO2
-
?

Synonyms

Synonyms Comment Organism
KdcA
-
Lactococcus lactis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
61
-
wild-type, melting temperature Lactococcus lactis
64
-
mutant V461I, melting temperature Lactococcus lactis
66.5
-
mutant V461I/A290M, melting temperature Lactococcus lactis
70.5
-
mutant V461I/A290M/Q252N/D306G/E316R/F388Y, melting temperature Lactococcus lactis
74.5
-
mutant V461I/A290M/Q252N/D306G/E316R/F388Y/L434C, melting temperature Lactococcus lactis
74.5
-
mutant V461I/A290M/Q252N/D306G/E316R/F388Y/L434C/M290C, melting temperature Lactococcus lactis
74.5
-
mutant V461I/A290M/Q252N/D306G/E316R/F388Y/L434C/M290V, melting temperature Lactococcus lactis
75.5
-
mutant V461I/A290M/Q252N/D306G/E316R/F388Y/I404C, melting temperature Lactococcus lactis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.2
-
3-methyl-2-oxobutanoate mutant V461I/A290M/Q252N/D306G/E316R/F388Y/I404C, pH 6.5, 30°C Lactococcus lactis
43
-
3-methyl-2-oxobutanoate mutant V461I/A290M/Q252N/D306G/E316R/F388Y/L434C/M290V, pH 6.5, 30°C Lactococcus lactis
69
-
3-methyl-2-oxobutanoate mutant V461I/A290M/Q252N/D306G/E316R/F388Y/L434C/M290C, pH 6.5, 30°C Lactococcus lactis
95
-
3-methyl-2-oxobutanoate mutant A290M/Q252N/D306G/E316R/F388Y/L434C, pH 6.5, 30°C Lactococcus lactis
118
-
3-methyl-2-oxobutanoate mutant V461I/A290M, pH 6.5, 30°C Lactococcus lactis
120
-
3-methyl-2-oxobutanoate mutant V461I, pH 6.5, 30°C Lactococcus lactis
145
-
3-methyl-2-oxobutanoate wild-type, pH 6.5, 30°C Lactococcus lactis
149
-
3-methyl-2-oxobutanoate mutant V461I/A290M/Q252N/D306G/E316R/F388Y, pH 6.5, 30°C Lactococcus lactis